Artigos e outras publicações

The association between body composition and PPARγ2 Pro12Ala polymorphism in a population with similar characteristics. 

Raquel S. SILVA, Darlene C. PERSUHN, Rogério P. FÉLIX, Filipe S. MARTINS, Franciele S. FERNANDES, Francisca K. BARBOSA, Gisele A. FRANCA, Joane R. BATISTA, Carolina F. LOPES, Glêbia A. CARDOSO , Alexandre S. SILVA

DOI: 10.23736/S0393-3660.24.05794-2

BACKGROUND: The Pro12Ala polymorphism in the PPARγ2 gene has been associated with body composition, however the results are conflicting. A possible cause of these conflicts is that covariates such as nutritional intake, physical activity and demographic factors were not considered. Thus, we verified the association between the Pro12Ala and body composition in individuals with similar characteristics. METHODS: A cross-sectional study was conducted with 37 postmen (39.3±10.9 years). The genotyping of the Pro12Ala was performed using the PCR-RFLP technique and the body composition measured by bioimpedance (InBody 720®). Energy expenditure was based on the compendium of physical activities and caloric intake was measured by 24 h recall questionnaire. RESULTS: There was a predominance for the Pro/Pro genotype (81.1% versus 18.9% Pro/Ala). Pro/Pro and Pro/Ala groups had respectively 610.0±290.6 and 496.4±256.7 kcal/day (P<0.34) of work activities, 150.0±21.2 and 11.4±22.7 minutes of leisure-time physical activity (P<0.73), and caloric intake of 2300.1±506.4 and 2242.2±472.8 (P<0.78), fat percentage (% FAT) of 24.9±7.6 and 21.3±7.7 (P<0.27), BMI of 27.2±4.8 kg/m2 and 25.7±3.4 kg/m2, with no differences between groups. According to regression analysis fat intake was influential for BMI and waist-hip ratio (WHR), while carbohydrate intake influenced WHR and %FAT. When considering extra-work physical activity, only fat intake influenced BMI. CONCLUSIONS: Pro12Ala does not influence body composition in a homogeneous population, in terms of age, job type, leisure time and socioeconomic level.

Habits Related to Strength Training of Brazilian Recreational Runners

Wagner K. A. Santos, Lucas D. M. Forte, Alexandre S. Silva, Hallisson V. de O. Rufino, Lucas de F. Vieira, João M. F. Lima Silva, Mabliny Thuany, Reginaldo Gonçalves  and Ytalo M. Soares

DOI: 10.3390/13010003

Despite strength training (ST) being well characterized by professional runners, little is known about the inclusion of ST models for recreational runners. Thus, the present study aimed to investigate the presence of ST in the training practices of recreational runners, with a focus on understanding its characteristics and the motivations of recreational athletes for including ST in their routines. To this end, 801 recreational runners (493 male and 308 female) completed a structured questionnaire regarding their training habits, the inclusion of ST, and its characteristics, concerning the type of ST, training volume, and the reasons for including ST in their training programs. To assess the possible associations between categorical variables, data were analyzed using the chi-square test for independent samples. Approximately 625 runners (78.1%) reported that they included ST in their training routine, with a statistically significant difference between the sexes (men: 73.5% vs. women: 85.4%; X² = 14.09; p = 0.01). Traditional strength training (TST) was the predominant type of ST included (78.5%), with most participants performing 2–4 sets and 8–12 repetitions per set at a frequency of 3–4 sessions per week. The importance of ST was primarily attributed to performance improvement (85%). The results suggest that recreational runners incorporate different types of ST into their training routines, with TST being the predominant type perceived by runners as a valuable asset for improving running performance.

Primary Dysmenorrhea Induced Using Diethylstilbestrol and Oxytocin Induces Impaired Uterine Reactivity in Virgin Female Wistar Rats

Francisco Fernandes Lacerda-Júnior 1,Petruska Pessoa da Silva Souza 2, Paula Benvindo Ferreira 1, Anderson Fellyp Avelino Diniz 3, Bárbara Cavalcanti Barros 1, Maria da Conceição Correia Silva, Adriano Francisco Alves 1,5, Alexandre Sérgio Silva 6 and Bagnólia Araújo da Silva 

DOI: 10.3390/ph18081191 

Background/Objectives: Primary dysmenorrhea (DysP) is a prevalent gynecological condition characterized by painful uterine contractions. However, the underlying mechanism of action of dysmenorrhea has not been fully elucidated. This study aimed to standardize an animal model of dysmenorrhea using diethylstilbestrol and oxytocin to mimic pathophysiological mechanisms in female Wistar rats. Methods: For the induction of dysmenorrhea, diethylstilbestrol (s.c.) and oxytocin (i.p.) were used. Results: The model effectively reproduced hypercontractility and impaired uterine relaxation. The in vivo evaluations demonstrated increased pain responses (DysP group = 119 ± 6.9; control group CG = 3.0 ± 1.0), which were partially attenuated by standard medications (scopolamine/dipyrone and ibuprofen). In vitro assays revealed greater contractile reactivity when compared to that in the control group, in the DysP group, using oxytocin (pEC50 = 3.6 ± 0.2 and Emax = 145.1 ± 8.7; CG (pEC50 = 3.1 ± 0.1 and Emax = 100%); KCl (DysP pEC50 = 2.2 ± 0.1 and Emax = 164 ± 8.0); CG (pEC50 = 1.8 ± 0.1) and PGF2α (DysP pEC50 = 7.4 ± 0.2 and Emax = 127.3 ± 15.6); CG (pEC50 = 6.2 ± 0.1)), while the relaxation responses to isoprenaline and nifedipine were decreased compared to those in the CG. The model promoted an imbalance in oxidative stress by increasing malondialdehyde (MDA) levels and reducing the total antioxidant capacity (TAC) in the uterine tissue. Conclusions: These findings suggest that the new virgin rat model is capable of replicating key aspects of the clinical features of DysP in humans and offers a valuable tool for studying its pathogenetic mechanisms and testing potential therapeutic agents.

GRAPE JUICE MINIMIZES INFLAMMATION AND REDUCES HOSPITALIZATION TIME IN PATIENTS WITH COVID-19

Crislane de Moura Costa, Alexandre Sergio Silva, Aislan Erick Pereira de Sousa, Rayane Carvalho de Moura, Valmir Oliveira Silvino, Aline Camarão Telles Biasoto, Débora Santos Lula Barros, Esmeralda Maria Lustosa Barros, Leonardo da Rocha Sousa, Joyce Lopes Macedo, Lais Lima de Castro Abreu, Maria do Carmo de Carvalho e Martins, Guilherme Loureiro Werneck, Davyson de Lima Moreira, Regis Bernardo Brandim Gomes, Marcos Antonio Pereira dos Santo.

DOI: 10.16891/2317

Inflammation and oxidative stress are the main mortality mechanisms caused by SARS-CoV-19. Grape juice has anti-inflammatory and antioxidant properties, which are believed to be beneficial in

treating COVID-19. This randomized clinical trial evaluated the impact of grape juice supplementation in the treatment of patients with COVID-19. Thirty-six patients were randomized to the grape juice supplementation group (GJG, n = 20; 10 ml/kg/day) or the control group (CG, n = 16), which received conventionalCOVID-19 treatment protocols. Blood samples were collected at admission and discharge for analysis of oxidative stress markers. CBC, C-reactive protein levels, electrolytes and markers of renal function were obtained from medical records. Handgrip strengthwas also measured to assess muscle strength. The GJG presented significantly lower levels of C-reactive protein compared to the CG (16.35 ± 26.81 vs 56.51 ± 73.29, respectively) with an intragroup decrease in C-reactive protein after the procedures (p = 0.010). The strength of the non-dominant hand in the GJG improved in relation to pre-intervention levels and in the CG after the procedures (p < 0.05), there was a significantly shorter time of hospitalization for the GJG compared to the CG (6.85 ± 3.16 vs 9.94 ± 5.69 days, respectively). The addition of grape juice during COVID-19 treatment reduced inflammation levels and length of hospital stay, as well as increased upper limb functional strength in COVID-19 patients. Clinical trial registration: RBR-4bcp54k

POST-EXERCISE HYPOTENSION AFTER MAXIMAL INTENSITY EXERCISE AND THE EFFECT OF BEETROOT ON BLOOD PRESSURE RESPONSE: A RANDOMIZED CLINICAL TRIAL

Ana Carla Lima de França, Joseline Lima e Silva Pinho, Carla Nunes dos Anjos, Bruno Rafael Virginio de Sousa, Klécia de Farias Sena, Lydiane Tavares Toscano, Eder Jackson Bezerra de Almeida Filho, Matheus da Silveira Costa, Aline Camarão Telles Biasoto, Alexandre Sérgio Silva

DOI/link:https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1184728/1/Post-exercise-hypotension-after-maximal-inten-2025.pdf

Abstract: Purpose: We investigate the effects of a running exercise session until exhaustion on the post-exercise pressure response and to see if beetroot changes this response. Materials and Methods: Normotensive recreational male street runners (39.2 ± 8.6 years, n= 17) performed two running to exhaustion at 80% of VO2 max: 1-preceded by intake of 140mL of beetroot juice (BET); or 2-control drink (CON). Measurements of blood pressure (BP) were performed at rest, immediately after exercise and every 10 min during 60 min recovery after exercise. Nitrite, malondialdehyde and total antioxidant capacity were measured before, 2 hours after ingestion of drinks and after exercise. Results: The BP decreased in both procedures at 60 minutes post-exercise in relation to the pre-exercise moment: systolic BP (BET: 108.9 ± 8.0 to 95.4 ± 5.1, p <0.05; CON: 109.4 ± 8.5 to 93.4 ± 5.3, p <0.05); diastolic BP (BET: 76.8 ± 6.2 to 66.4 ± 1.5 p <0.05; CON: 75 ± 65.4 to 65.5 ± 1.0, p <0.05), with no difference between procedures. Conclusion: Exercise until exhaustion promotes a significant reduction in BP, but intake of a single dose of beetroot juice before exercise does not promote additional effects on the post-exercise hypotensive response in recreational runners. Trial registration number and date of registration: RBR-9ydn4bn, June 09, 2022. 

Hepatoprotective, Lipid-Lowering and Antioxidant Effects of Mangaba Powder (Hancornia speciosa) Administered to Rats Fed a High-Fat Diet.

Bernadete de Lourdes de Araújo Silva, Margarida Angélica da Silva Vasconcelos, Kamila Sabino Batista, Marcos dos Santos Lima, Fabiane Rabelo da Costa Batista, Hassler Clementino Cavalcante, Lydiane de Lima Tavares Toscano, Alexandre Sérgio Silva, Aline Barbosa D’Oliveira,  Adriano Francisco Alves, Jailane de Souza Aquino.

DOI: 10.3390/foods14111993

The aim of this study was to evaluate the potential effects of administering mangaba powder on liver function and somatic, oxidative and lipid metabolism parameters in rats fed a high-fat diet. Prepared mangaba powder has important amounts of phenolic compounds, vitamin C, dietary fiber and oligosaccharides. A total of 32 adult Wistar rats were initially randomized into two groups for the biological assay: normal-fat (NF, n = 16) and high-fat (HF, n = 16) diets for 21 days. These rats were subsequently subdivided into four groups: NF (n = 8), HF (n = 8), normal-fat diet with mangaba powder administration (NFMG, n = 8) and high-fat diet with mangaba powder administration (HFMG, n = 8). The treatment with mangaba powder (400 mg/kg) lasted an additional 28 days. Compared to the HF rats, the HFMG rats showed an 8% reduction in the body mass index. Treatment with mangaba reduced the serum cholesterol by 18%, as well as the hepatic deposition of triacylglycerides by 26% and cholesterol by 25%, in addition to increasing bile acid synthesis by 77% in this organ. Mangaba powder consumption attenuated the degree of hepatic steatosis, reduced lipid peroxidation and increased the serum and hepatic antioxidant capacity in HFMG rats. These results show that the consumption of mangaba powder had lipid-lowering, hepatoprotective and antioxidant effects, especially in HFMG rats, which may be associated with an additive and synergistic action between the bioactive compounds present in the product.

Association Between Adiposity Level, Blood Pressure, Physical Activity and Cardiac Autonomic Dysfunction: A Population-based Study

Rayane Carvalho de Moura, Edna Maria Silva Araújo, Crislane De Moura Costado, Alexandre Sergio Silva, Jefferson Cruz dos Santos Leite, Sergio Luis Galan Ribeiro, Cirley Pinheiro Ferreira, Valmir Oliveira Silvino, Marcos Antonio e Pereira dos Santos.

DOI: 10.2174/0115734013355912250414063407

Introdução: A obesidade e a hipertensão arterial são problemas de saúde complexos e multifatoriais que representam sérios problemas de saúde pública. A atividade física é um poderoso fator de proteção no tratamento dessas comorbidades. A disfunção autonômica cardíaca tem demonstrado forte associação com fatores de risco cardiovascular e diversas outras comorbidades.

Objetivo: Associar o nível de adiposidade, a pressão arterial, a atividade física e a disfunção autonômica cardíaca.

Métodos: Este estudo transversal de base populacional incluiu 285 indivíduos (idade média: 45,1 ± 16,3 anos) de ambos os sexos. Foram avaliadas a composição corporal, a pressão arterial e a variabilidade da frequência cardíaca, juntamente com os níveis de atividade física. Foram realizados testes de correlação de Spearman e qui-quadrado. Para a análise de associação, utilizou-se regressão logística binária (razão de chances).

Resultados: Observou-se uma associação significativa entre adiposidade corporal e pressão arterial sistólica e diastólica em ambos os sexos (p=0,000). Além disso, foi encontrada uma associação significativa entre circunferência da cintura e atividade do sistema nervoso simpático na amostra total (p=0,003). Homens com circunferência da cintura elevada apresentaram maior probabilidade de ter baixos níveis de atividade parassimpática, conforme indicado pela variável SDNN (desvio padrão de todos os intervalos NN) (p=0,002). Em relação à atividade física, houve uma associação significativa com a pressão arterial. Após ajuste para idade, indivíduos insuficientemente ativos apresentaram maior probabilidade de desenvolver hipertensão arterial (p=0,08).

Conclusão: Existe uma associação entre alta adiposidade corporal e baixos níveis de atividade física com aumento da pressão arterial, e o aumento da circunferência abdominal está ligado a um maior risco de disfunção autonômica, elevando assim o risco de doenças.

Effects of pre-psychological assessment (IPC) on neuromuscular and biochemical changes in paralympic athletes during the training period: Protocol study

Halekson B. de Freitas, Marlon A. de Albuquerque, Alexandre Sérgio Silva, Herik V. C. Fonseca, Eriklys C. Barreto, Heleodório Honorato Santos 

DOI: 10.17784/mtprehabjournal

Background: Ischemic pre-conditioning (IPC) is one of the forms of imposing this ischemia, alternating complete vascular occlusion and reperfusion before exercise, to pre-condition varied physiological functions. In the sporting context, there are moments when an athlete interrupts his physical activities, resulting in a reduction in strength and muscle mass. Accordingly, a hypothesis arises that the IPC will be able to reduce training effects, speeding up the process of the athlete’s return to his sporting activities. 

Objective: To evaluate the effects of IPC on various neuromuscular forces (isometric force, explosive force and electromyography) and markers of oxidative stress and muscle damage (Creatine Kinase, Malondialdehyde and antioxidant capacity), in paralympic athletes, subject to periods of discontinuity from training loads. 

Methods: The sample will be composed of 38 young adult athletes (20 to 40 years old), of both sexes, who will compete in competitions at a national level in their own way. These will be randomly divided into 2 groups: experimental (IPCG: n = 19), and control (CG: n = 19). The GIPC will be subject to 4 weeks of IPC twice a day (40 weeks). Both groups will be validated before, and after 2 and 4 weeks of surgery, as regards: body composition, lower limb strength, explosive strength and electromyography (EMG), as well as biochemical markers Creatine Kinase (CK), Malondialdehyde (MDA) and antioxidant capacity (CAT). These data will be analyzed in the Statistical Package for the Social Science (SPSS – 25.0). Initially, we will test the normality (Shapiro-Wilk test), homogeneity (Levene test) and sphericity (Mauchly test) of the data, followed by ANOVA (repeat tests) to analyze the effects of the protocols, before and after 2 and 4 weeks of intervention followed Bonferroni’s post hoc tests, adopting a significance level of P  0.05, in all analyses. Expected results: We hope that the GIPC intervention will alleviate or inhibit the reduction of strength and muscular hypotrophy in our athletes during the period of discontinuation of training loads.

Thermogenic supplement attenuates post-exercise hypotension after aerobic exercise in normotensive young adults without affecting heart rate variability

Douglas Cavalcante Silva, Reabias de Andrade Pereira, Gustavo da Silva Félix, Marizângela Ferreira de Souza, Glêbia Alexa Cardoso, George Celso Souza Côrtes de Araújo, Marcos Antonio Pereira dos Santos, Alexandre Sérgio Silva.

DOI: 10.1007/s11332-025-01546-z

Background

It is well-established that exercise promotes post-exercise hypotension (PEH), a reduction in blood pressure following physical exercise. However, thermogenic substances may induce vasoconstriction, tachycardia, and increased blood pressure, potentially abolishing PEH. Aim To investigate the impact of the use of thermogenic supplements on blood pressure (BP) response and heart rate variability (HRV) after exercise.

Methods

Twenty healthy normotensive young adults (25 ± 6 years), participated in a randomized, placebo-controlled, crossover trial. Each completed two aerobic exercise sessions (60 min at 60 − 85% of maximal heart rate), preceded by ingestion of thermogenic supplement (LIPO-6 Black Ultra Concentrated) or placebo (starch), taken 30 min before exercise. BP and HRV were measured before supplementation, 30 min post-supplementation/pre-exercise (every 10 min), and for 60 min post-exercise (BP every 15 min; HRV every 30 min).

Results

In placebo condition, systolic/diastolic BP decreased at 15 (− 3.3/ − 2.1 mmHg), 30 (− 4.2 / 0.8 mmHg), 45 (− 6.5/ − 2.1 mmHg) and 60 (− 6.3/0.4 mmHg) minutes post-exercise. In thermogenic condition, BP increased at 15 (2.6/2.1 mmHg), 30 (1.3/2.5 mmHg), 45 (1.0/3.4 mmHg) and 60 (1.0/5.6 mmHg) minutes. ANOVA revealed significant condition × time interactions for systolic (p < 0.01; η2p = 0.19) and diastolic BP (p < 0.04; η2p = 0.14). A significant increase in pNN50 was observed in the thermogenic condition (12.9 ± 10 ms to 24.8 ± 18 ms; p < 0.01) 30 min post-supplementation, with no significant change in placebo. RMSSD, LF, and HF remained unchanged.

Conclusion

Thermogenic supplementation abolished PEH but did not significantly affect HRV in healthy normotensive young adults.

Does the energy expenditure associated with training influence reduced adiposity in practitioners of exercise? Data from a cross-sectional population study

Raquel Suelen B DA Silva, Marizângela F DE Souza, Matheus DA S Costa, Joana Raquel E Batista, Gisele Augusta M Franca, Francisca Karinny L Barbosa, Carolina FA Lopes, Glêbia A Cardoso, Alexandre S Silva.

DOI: 10.1590/0001-3765202520240058

Meta-analyses have shown modest weight loss with physical training. Low training load can be one possible cause, however this has not yet been investigated. This cross-sectional study investigated the correlation between training load parameters and the body composition of 335 individuals engaged in exercise (47.9 ± 12.7 years, 138 males). Data on exercise frequency, intensity, duration, and body composition were collected. Men showed significantly higher energy expenditure during training (632.5, 95% CI: 560.0 – 704.5) compared to women (280.4, 95% CI: 253.9- 306.8). Greater energy expenditure was inversely correlated with lower body mass index (r=-0.23; p<0.05), body fat percentage (BF%) (r=-0.53; p<0.05), fat mass (r=-0.37; p<0.05), waist-to-hip ratio (r= -0.31; p<0.05), and visceral fat (r=-0.38; p<0.05) in both sexes. Significant correlations persisted when considering confounding factors among men, except for skeletal muscle mass. For women, only BF% and skeletal muscle mass correlations remained. Linear regression analysis indicated that to attain a BF% below 20%, individuals needed to expend 9.07 kcal/kg per training session in seven weekly sessions (r2= 0.33); however, only 15.2% of the participants met this energy demand. Thus, an adequate maintenance of adequate body fat levels appears to be dependent on individuals with a high energy expenditure during training, without significant reliance on other influencing factors.

Sleep quality and specific characteristics of different types of impairments in Paralympic athletes: an updated systematic review

Aldrim Pontes Araújo, Mário Antônio de Moura Simim, Thiago Fernando Lourenço, Filipe Santana Martins, Alexandre Sérgio Silva, Marco Túlio de Mello, Andrea Maculano Esteves, Andressa Silva, Gabriella Andreeta Figueiredo, João Gustavo Claudino

DOI: 10.1007/s40675-025-00351

Objetivo da Revisão: Identificar e descrever os instrumentos e parâmetros utilizados na literatura científica para monitorar a qualidade do sono em atletas paralímpicos, bem como investigar se a seleção ou aplicação dessas ferramentas de monitoramento considera as características específicas de diferentes tipos de deficiências. Resultados Recentes: Com base em uma revisão da literatura atual: a má qualidade do sono é altamente prevalente; os fatores contribuintes são estresse, carga de treinamento e desalinhamento circadiano; ajustes no treinamento, gerenciamento da carga e redução gradual do treinamento são eficazes; instrumentos mais adaptados são necessários. Embora uma variedade de instrumentos seja utilizada para monitorar o sono em atletas paralímpicos, a maioria dos estudos carece de adaptações metodológicas que considerem as especificidades funcionais desses atletas. A predominância de estudos transversais e os tipos de deficiências revelam limitações na profundidade da pesquisa. A escassez de adaptações dos instrumentos compromete a validade dos dados e destaca a necessidade de protocolos mais específicos para atletas paralímpicos.

Synergistic effect of ozone treatment with α-amylase on the modification of microstructure and paste properties of japonica rice starch

Rafael Lucas Jacinto Almeida, Newton Carlos Santos, Shenia Santos Monteiro, Shirley Santos Monteiro, João Vítor Fonseca Feitoza, Mércia Mélo de Almeida Mota, Rafael da Silva Eduardo, Patrícia Marinho Sampaio, Gilsandro Alves da Costa, Matheus Augusto de Bittencourt Pasquali, Rebeca de Almeida Silva, Flávia Izabely Nunes Moreira, Lyandra Maria de Oliveira, Tamires Dos Santos Pereira, Artur Xavier Mesquita de Queiroga, Celene Ataíde Cordeiro Ribeiro.

DOI: 10.1016/j.foodchem.2024.142145

Resumo

O objetivo foi avaliar o efeito sinérgico da ozonização e da α-amilase na modificação da microestrutura e das propriedades da pasta de amido, utilizando 0,00042 g de ozônio/100 g de tampão por diferentes períodos. A susceptibilidade enzimática aumentou, atingindo valores máximos de 12,73%, com um aumento de 11,42% na cristalinidade e um tamanho médio de partícula de 10,12 μm para amido de arroz japonica (JR90) tratado por 90 minutos. Os grânulos apresentaram formato poliédrico e, com o aumento da intensidade dos tratamentos combinados, formaram aglomerados e perderam sua geometria original. A viscosidade aparente, os parâmetros reológicos e texturais foram reduzidos devido à ação mais eficiente da α-amilase sobre o amido ozonizado, conforme confirmado pelo baixo valor da entalpia de gelatinização (7,61 J/g). O ozônio mostrou-se eficaz na abertura das cadeias de amido, na gelatinização parcial dos grânulos, na homogeneização do meio enzimático e no aumento da taxa de hidrólise da α-amilase no amido do arroz japonica.

Digestibility properties and supramolecular structure of starch prepared by high hydrostatic pressure and α-amylase treatment

Almeida, Raphael Lucas Jacinto Santos, Newton Carlos Monteiro, Shenia Santos Monteiro, Shirley Santos Albuquerque, Juliana Cruz da Silva Eduardo, Raphael de Andrade Freire, Vitoria da Silva, Andre Miranda de Oliveira, Lyandra Maria de Queiroga, Artur Xavier Mesquita de Sousa, Alison Bruno Borges da Costa, Gilsandro Alves de Figueiredo, Maria Jose Ribeiro, Celene Ataide Cordeiro de Almeida Silva, Rebeca

DOI: 10.1007/s00217-025-04799-7

Riceberry starch, a promising ingredient for functional foods, has limited digestibility and functionality in its native form. While high hydrostatic pressure (HHP) and enzymatic treatment are known to modify starch properties, their combined effects on Riceberry starch remain underexplored. This study investigated the synergistic impact of HHP (200–600 MPa) and α-amylase treatment on the in vitro digestibility, morphostructural, thermal, and rheological properties of riceberry starch, aiming to identify optimal processing conditions for tailored starch functionality. Starch was treated at 200 MPa (α200), 400 MPa (α400), and 600 MPa (α600) for 10 min, followed by α-amylase hydrolysis (30 U mg−1) for 360 min. Compared to enzyme-only control (RBα), HHP above 200 MPa significantly reduced starch content and long-chain amylopectin branching. Digestibility varied with pressure: α200 showed higher rapidly digestible starch (RDS: 30.16%) and slowly digestible starch (SDS: 54.19%), whereas α600 yielded more resistant starch (RS: 38.22%). Microstructural analysis revealed larger clusters (112.69 µm) and lower specific surface area (0.25 m2 g−1) for α600, explaining its reduced digestibility. Although the crystalline structure (type A) was retained, relative crystallinity declined (α600: 24.88%). Higher HHP intensities (α600) increased thermal resistance to gelatinization and viscosity, demonstrating pressure-dependent rheological modifications. HHP at 200–400 MPa combined with α-amylase optimally balanced digestibility and functional properties, offering a targeted approach to tailor riceberry starch for food applications requiring specific thermal, rheological, or digestive profiles.

Impact of direct/indirect freezing and pulsed electric field-assisted thawing on the techno-functional properties of cassava starch (Manihot esculenta Crantz)

Rafael Lucas Jacinto Almeida, Newton Carlos Santos, Iêda Letícia de Souza Ferreira, Rafael da Silva Eduardo, André Miranda da Silva, Artur Xavier Mesquita de Queiroga, Maria José de Figueiredo, Celene Ataíde Cordeiro Ribeiro, Rebeca de Almeida Silva, Shirley Santos Monteiro, Gilsandro Alves da Costa, Thayse Cavalcante da Rocha, Alison Bruno Borges de Sousa, Mércia Mélo de Almeida Mota 

DOI: 10.1016/j.foodchem.2025.146827

O estudo examinou os efeitos de diferentes métodos de congelamento e descongelamento nas propriedades do amido de mandioca, comparando o congelamento direto (CD) com nitrogênio líquido a −196 °C com o congelamento indireto (CI) em um ultracongelador a −80 °C, seguido por descongelamento convencional a 25 °C ou descongelamento assistido por campo elétrico pulsado (PEF) a 12 kV/cm por 8 min. O CD causou menos danos estruturais aos grânulos de amido do que o CI devido à formação de cristais de gelo menores e mais uniformes. O PEF durante o descongelamento mostrou-se particularmente eficaz na preservação da qualidade do amido, mantendo a cristalinidade e a integridade molecular, além de prevenir a deterioração das propriedades térmicas e reológicas tipicamente causada pelo congelamento. O tratamento com PEF facilitou a reorganização molecular dentro da matriz do amido, reduzindo a retrogradação e aumentando a estabilidade geral. A combinação de métodos de congelamento com descongelamento assistido por PEF (DF12 e IF12) minimizou a degradação dos componentes amilose e amilopectina, com valores medidos para entalpia de gelatinização e parâmetros de viscosidade muito próximos aos da amostra de controle não tratada (P12).

Valorization of cabotiá pumpkin (Cucurbita maxima) by-products in the development of instant puree mixes: Nutritional, functional, and sensory evaluation

Mateus Sabino de Souza FERREIRA, Lukas Phellipe Guedes BARBOSA, Raphael Lucas Jacinto ALMEIDA, Shênia Santos MONTEIRO, Sinara Pereira FRAGOSO, Carolina Lima Cavalcanti de ALBUQUERQUE, Matheus Augusto de Bittencourt PASQUALI, Stela de Lourdes Ribeiro de MENDONÇA, 

Yuri Montenegro ISHIHARA, Celene Ataíde Cordeiro RIBEIRO, Maria José de FIGUEIREDO, Neila Lidiany RIBEIRO, Gilsandro Alves da COSTA

DOI: https://www.fstjournal.com.br/revista/article/view/548/399

The  aim  of  this  study  was  to  valorize  the  waste  from  cabotiá  pumpkin  (peel  and  seeds)  and  use  them  to  develop  an instant  puree  mix,  as  well  as  to  evaluate  its  nutritional,  functional,  and  sensory  properties.  Three  formulations  were 

developed:  F1:  Pumpkin  puree  with  milk,  salt,  and  coloring;  F2:  Pumpkin  puree  with  pumpkin  peel,  added  with milk,  salt,  and  coloring;  and  F3:  Pumpkin  puree  with  both  peel  and  seeds,  added  with  milk,  salt,  and  coloring.  The formulations  were  characterized  in  terms  of  nutritional,  physicochemical,  structural,  and  morphological  properties, as well as bioactive compounds, bioaccessibility, antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic  acid,  and  ferric  reducing  antioxidant  power,  and  microbiological  and  sensory analysis.  The  addition  of  cabotiá  pumpkin  waste  resulted  in  increased  levels  of  proteins,  lipids,  flavonoids,  and  total phenolic  compounds,  which  contributed  to  higher  antioxidant  activity  and  bioaccessibility,  as  well  as  reduced  sugar levels in the ready mixes. Microbiological analyses confirmed the food safety of the ready mixes. F1 and F2 received a score of 7.1 in sensory analysis, but F2 had a more pronounced flavor profile, making it more appealing to the tasters and increasing purchase intent. The addition of pumpkin peel flour enhanced consumer acceptance, possibly due to the presence of antioxidant and aromatic compounds. It was found that the ready mixes can be marketed as functional or nutraceutical foods of quick preparation, contributing to the reduction of agro-industrial waste.

Synergy between chloride salts (Na+, K+, Ca2+) and ozonation in the simultaneous modification of pigmented rice starch

Rafael Lucas Jacinto AlmeidaNewton Carlos Santos, Iêda Letícia de Souza Ferreira, Rafael da Silva Eduardo, Vitória de Andrade Freire, Maely Jezzer Cândido Venâncio, Lucas Wallyson Pereira de Queiroz, Osmar Batista de Souza Neto, Alison Bruno Borges de Sousa, Ellen Cristina Nabiça Rodrigues, Elizabete de Oliveira Araújo, Mateus Sabino de Souza Ferreira, Gilsandro Alves da Costa, Maria José de Figueiredo, Celene Ataíde Cordeiro Ribeiro 

DOI: 10.1016/j.foodchem.2025.147087

This study evaluated the dual chemical modification of red rice starch through a synergistic combination of ozonation and chloride salts (NaCl, KCl, and CaCl₂). A 2-h ozonation time (0.045 g/L and 50 g/h) and a fixed salt concentration of 200 mM were employed. The carbonyl and carboxyl contents increased with the addition of salt, as did the breakdown of starch chains, amylose, and larger branches of amylopectin, especially when using KCl. Crystallinity was reduced by 10.68 % of the starch granule, resulting in greater surface erosion and a reduction in the average diameter to 33.71 μm with KCl. The larger atomic radius of K+ led to stronger electrostatic interactions, reducing the enthalpy to 7.33 J/g and producing textural properties similar (p > 0.05)  to those obtained with CaCl2.

Evaluation of IL6 Promoter Methylation in Adults with COVID-19 and Pediatric Leukemia Patients: The Role of Propolis and Methotrexate in Epigenetic Modulation

José Nunes de Queiroz Neto; Cássia Surama Oliveira da Silva; Mayara Karla dos Santos Nunes; Mussara Gomes Cavalcante Alves Monteiro; Darlene Camati Persuhn; José Maria Chagas Viana Filho; Francisco Eduardo de Carvalho Costa; Maria José de Carvalho Costa; Naila Francis Paulo de Oliveira

DOI: 10.1007/s40142-025-00216-3

The natural active ingredient propolis, which is used to support the treatment of inflammatory diseases, and the drug methotrexate (MTX), which is used to treat tumor diseases, have already shown that they are able to change the DNA methylation profile. The aim of the study was to evaluate the ability of propolis oral spray and intravenous methotrexate to modulate the DNA methylation profile of the interleukin-6 (IL6) gene, which encodes an inflammatory cytokine that is important in both inflammatory and tumor processes. The propolis intervention was tested in adults affected by COVID-19 (n = 114) and the methotrexate intervention was tested in children and adolescents with leukemia (n = 64). DNA methylation in the IL6 gene promoter was analyzed using the methylation-specific PCR (MSP) technique using DNA extracted from oral mucosa or blood. The fully methylated profile in blood cells of adults with COVID-19 was observed in 100% of the samples both before and after the propolis intervention. The methylated profile was also observed in buccal cells from pediatric patients with leukemia in 100% of the samples regardless of MTX treatment. It is concluded that the methylated profile in the IL6 gene promoter is a common profile for adult blood cells and pediatric oral cells and is not modulated by the use of propolis or methotrexate.

Autochthonous strains of Lactiplantibacillus plantarum and Levilactobacillus brevis with technological and bioactive potential as starter cultures for sourdough production

Ana Regina Simplício de Medeiros, Noádia Priscila Araújo Rodrigues, Marcus Vinicius de Souza Couto, Gabriel Victor Pinheiro Barbosa, Tatiana Zanella Rodrigues, Ingrid Conceição Dantas Gonçalves, Eloiza Helena Campana d, Ana Luiza Mattos Braga e, Marcos dos Santos Lima f, Maristela Alves Alcântara, Angela Maria Tribuzy de Magalhães Cordeiro, Estefânia Fernandes Garcia

DOI: 10.1016/j.ijgfs.2025.101221

This study investigated sourdoughs from different climatic regions in northeastern Brazil to identify autochthonous strains of lactic acid bacteria (LAB) and evaluate their technological potential. Sourdough samples were collected from humid, sub-humid, and semi-arid regions, yielding 129 bacterial isolates. Of these, 24 strains exhibited typical LAB characteristics and were identified via MALDI-TOF mass spectrometry as Lactiplantibacillus plantarum, Companilactobacillus paralimentarius, and Levilactobacillus brevis. C. paralimentarius dominated in humid regions, while L. plantarum prevailed in other climates. From principal component analysis, seventeen strains with good technological potential were selected based on acidification, proteolytic activity, and exopolysaccharide production. These strains then underwent tolerance tests for acid, salt, sucrose, and ethanol stress. L. plantarum 47 (Lp47) exhibited superior acid and salt tolerance. L. brevis 83 (Lb83) presented tolerance to high ethanol concentrations. These strains were then used as starter cultures to evaluate fermentative performance. Lp47 and Lb83 demonstrated rapid growth and acidification under fermentation stress, respectively acidifying the medium at 4 and 6 h. Both strains progressively enhanced antioxidant activity and phenolic compound production during fermentation, with no significant differences (p > 0.05). Rheological tests revealed that sourdoughs with Lp47 and Lb83 were less sticky, more elastic, and exhibited greater deformation resistance after 24 h. These findings underscore the potential of Lp47 and Lb83 as effective starter cultures for sourdough production, ensuring faster, controlled fermentation while enhancing textural and bioactive properties, particularly for large-scale applications. Exploring the biotechnological potential of sourdough fermentation processes expands our understanding and opens new possibilities in gastronomy and food science for developing functional, healthy and high value food products. Our study highlights the importance of autochthonous strains in enhancing fermentation. These strains accelerated production, improved efficiency, and enhanced bread quality, demonstrating their potential to significantly impact the food industry.

Microencapsulated phenolic compounds from organic coffee husk: Impacts on human gut microbiota and in vitro prebiotic potential

Gezaildo Santos Silva, Flavia Izabely Nunes Moreira, Thatyane Mariano Rodrigues de Albuquerque, Thaianaly Leite Abreu, Eike Guilherme Torres de Souza, Layane Rosa da Silva, Antonia Dayane Jenyffer de Farias Marques, Mercia de Sousa Galvao, Marcos dos Santos Lima d , Evandro Leite de Souza, Marta Suely Madruga, Louise Emy Kurozawa, Taliana Kˆenia Alencar Bezerra

DOI:10.1016/j.foodres.2024.115597

This research investigated the influence of the microencapsulation of phenolic compounds (PCs) from organic coffee husk with whey protein concentrate (WPC) and maltodextrin on the abundance of intestinal bacterial populations and their metabolic activity during in vitro fecal fermentation. The microencapsulated PCs were gradually metabolized during fecal fermentation, resulting in significant transformations and an increase in PCs in the fermentation media. The metabolism of PCs by the fecal microbiota occurred concurrently with the consumption of sugars, production of organic acids, and reduction in pH in the media. The PCs, especially when encapsulated, promoted an increase in the abundance of Lactobacillus spp./Enterococcus spp., Bifidobacterium spp., and Ruminococcus albus/R. flavefaciens, and a reduction in the abundance of Bacteroides spp./Prevotella spp., Clostridium histolyticum, and Eubacterium rectale/Clostridium cocoides. The results suggest that the PCs exhibited prebiotic potential, with their efficacy enhanced by microencapsulation, particularly when WPC was used exclusively as the encapsulating agent.

Supplementation with Lactiplantibacillus plantarum CNPC003 and Pilosocereus gounellei Flour Enhances the Properties of Goat Cream Cheese

Daniela Karla Medeiros Vasconcelos, Evandro Leite de Souza2, Márcia Gabrielle Silva Viana, Maria Isabel Ferreira Campos, Lorena Lucena de Medeiros, Lary Souza Olegário, Mércia de Sousa Galvão, Karina Maria Olbrich dos Santos, Antônio Silvio do Egito, Marta Suely Madruga, Marcos dos Santos Lima, Tatiana Santi Gadelha, Maria Teresa Bertoldo Pacheco, Kataryne Árabe Rimá de Oliveira, Maria Elieidy, Gomes de Oliveira

DOI: 10.3390/microorganisms13020254

This study evaluated the impacts of Lactiplantibacillus plantarum CNPC003 and xique-xique flour supplementation on the technological, physicochemical, nutritional, and sensory properties of goat cream cheese over 21 days of refrigerated storage. Four cheese formulations were prepared: a control (CC), one with L. plantarum CNPC003 (PC), one with xique-xique flour (XC), and one with L. plantarum CNPC003 and xique-xique flour (PXC). XC and PXC had a yellowish-green hue with less brightness. PC and PXC were less firm and adhesive with greater elasticity, cohesiveness, and gumminess, and they had reduced total protein and increased total free amino acids (p < 0.05) during storage. The contents of specific volatile compounds increased in PXC during storage. PXC had higher L. plantarum counts than PC on day 21 of storage. PC and PXC had distinct colors and textures and were well accepted regarding sensory attributes. Xique-xique flour and L. plantarum CNPC003 supplementation positively impact the nutritional and functional characteristics of goat cream cheese without negatively affecting the technological and sensory attributes.

Antibacterial and Anti-virulence Properties of Cell-free Supernatant from a Mixture of Limosilactobacillus fermentum Strains Against Enterotoxigenic Escherichia coli

Maiara da Costa Lima, Yuri Mangueira do Nascimento, Evandro Ferreira da Silva, Josean Fechine Tavares, Marcelo Sobral da Silva, Evandro Leite de Souza 

DOI: 10.1007/s12602-025-10509-2

This study investigated the antimicrobial efficacy of a cell-free supernatant (CFS) and live cells from a mixture of Limosilactobacillus fermentum strains (139, 296, and 263). These strains are candidates for probiotic use and were evaluated against five STb virotype enterotoxigenic Escherichia coli (ETEC) strains. Antagonistic activities of L. fermentum mixed cells and CFS were assessed with the spot agar method. The impacts of CFS on viable cell counts in laboratory medium, biofilm formation, cell surface hydrophobicity, mucin, and motility were evaluated. The effects of CFS on ETEC viable cell counts under laboratory medium and during exposure to simulated gastrointestinal conditions were measured using plate count, and the physiological status of L. fermentum cells was assessed with flow cytometry. The nuclear magnetic resonance technique identified the metabolites from CFS and its effects on the ETEC metabolite profile. A larger growth inhibition zone was caused by L. fermentum viable cell mixture (18.4-35.8 mm) than CFS (10.1-30.1 mm). CFS caused viable cell count reductions of ≥ 5 log CFU/ml at 24 h of exposure and reduced biofilm formation, cell surface hydrophobicity, mucin, and swimming and swarming motility of ETEC strains. The exposure of ETEC strains to simulated gastrointestinal conditions resulted in viable cell counts < 2 log CFU/ml at the final digestion when supplemented with CFS. Exposure to CFS resulted in sizes of ETEC cell subpopulations characterized as dead or injured > 80.0%. Lactate and acetate were the most critical variables in CFS, directing decreased ETEC metabolic activity. Results showed that the examined CFS from L. fermentum 139, 263, and 296 mixture was an effective antimicrobial against ETEC.

Impact of a Limosilactobacillus fermentum, Quercetin, and Resveratrol Nutraceutical on Fecal Microbiota Composition and Metabolic Activity in Healthy and Hypertensive Subjects

Jéssica Maria Alves Brasil, Nathalia Caroline de Oliveira Melo, Karoliny Brito Sampaio, Paulo César Trindade da Costa, Hatice Duman, Sercan Karav, Marcos dos Santos Lima, Evandro Leite de Souza, José Luiz de Brito Alves

DOI:  10.3390/foods14060986

A promising strategy to improve the gut microbiome in hypertension is to target the gut microbiota. This study evaluated the effects of a potential nutraceutical product composed of three strains of Limosilactobacillus (L.) fermentum, quercetin, and resveratrol on the intestinal microbiome of healthy and hypertensive subjects. The nutraceutical product consisting of strains of L. fermentum 139, 263 and 296, fructooligosaccharides (200 mg), quercetin (160 mg), and resveratrol (150 mg) (LfQR) was added to the in vitro fecal fermentation process occurring for 48 h. Fecal samples of healthy and hypertensive subjects were allocated into four groups: (i) healthy controls (CTL); (ii) healthy controls with the addition of LfQR (CTL + LfQR); (iii) hypertensive (HTN) subjects; and (iv) hypertensive subjects with the addition of LfQR (HTN + LfQR). The diversity and composition of the fecal microbiota and the production of microbial metabolites were evaluated. CTL and HTN groups exhibited a distinct gut microbiota composition, as shown by the β-diversity assessment. The addition of the potentially nutraceutical-modulated β-diversity was similar between CTL and HTN groups, suggesting a similar gut microbiome composition after nutraceutical addition. The addition of the nutraceutical product increased the relative abundance of Enterobacteriaceae in the CTL group and that of Lachnospiraceae in the HTN group. The nutraceutical media showed higher levels of sugars (maltose, fructose, and glucose), lactic acid, ethanol, succinic acid, and acetic acid compared to the CTL and HTN media. Although the results are heterogeneous between healthy and hypertensive fermentation media, it was demonstrated that the nutraceutical formulation can modulate the intestinal microbiota and its metabolic activity.

A novel coating formulated with sodium alginate loaded with a mixed lactic acid bacteria culture to control anthracnose in guava and mango

Karina Felix Dias Fernandes, Kataryne Árabe Rimá de Oliveira, Ítallo Fernandes Cirilo, Maiara da Costa Lima, Marcos Paz Saraiva Câmara, Evandro Leite de Souza

DOI:10.1016/j.foodcont.2025.111341

Guava and mango are tropical fruits widely consumed due to their pleasant flavor and nutritional composition. Anthracnose caused by Colletotrichum spp. Is considered a primary post-harvest disease affecting these fruits. Applying biocontrol agents, such as lactic acid bacteria (LAB), combined with coating materials is an innovative fruit biopreservation method. This study investigated the effects of a novel sodium alginate coating loaded with a mixed culture of LAB in controlling anthracnose caused by different pathogenic Colletotrichum isolates in guava (cv.) Paluma and mango (cv.) Palmer during 15 days of room temperature storage (25 ± 0.5 ◦C). A mixed culture formed by three different LAB species strains (Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263) was tested against ten target Colletotrichum isolates. The mixed culture inhibited the mycelial growth of all target Colletotrichum isolates in vitro, with up to 100 % inhibition. In the nutrient competition method, the mixed LAB culture survived and caused mycelial growth inhibition even at low nutrient concentrations. The coatings showed sizes of LAB cell subpopulations with non-permeabilized membrane (viable cells, PI-CFDA+) of >31 % at the end of refrigerated storage and viable cell counts of >7 log CFU/ g. Applying coatings with sodium alginate loaded with mixed LAB culture delayed the development and decreased the severity of anthracnose lesions in guava and mango artificially contaminated with either of the tested Colletotrichum isolates. The coating formulated with sodium alginate and mixed LAB culture can be effective for postharvest anthracnose biocontrol in guava and mango.

Influence of Autochthonous Lactic Acid Bacteria Cultures on the Microbiota and Biogenic Amine Production in Medium-Ripened Artisan Goat Cheese

Julia Mariano Caju de Oliveira, Adriane Elisabete Costa Antunes, Gustavo Felipe Correia Sales, Camila Neves Meireles Costa, Angela Matilde da Silva Alves, Kaíque Yago Gervazio de Lima, Celso José Bruno de Oliveira, Antônio Silvio do Egito, Karina Maria Olbrich dos Santos, Evandro Leite de Souza, Maria Teresa Bertoldo Pacheco and Maria Elieidy Gomes de Oliveira

DOI:10.3390/foods14091561

This study evaluated the effects of adding the autochthonous cultures Limosilactobacillus mucosae CNPC007 (LM) and Lactiplantibacillus plantarum CNPC003 (LP), originally isolated from goat milk and goat cheese, respectively, on microbiological safety, microbiota composition (analyzed through 16S rRNA gene metataxonomic sequencing), and biogenic amine (BA) production in artisanal goat (coalho) cheese made from raw or pasteurized milk during 60 days of ripening at 10 ◦C. Six types of cheese were produced, varying in milk treatment (raw or pasteurized) and the presence or absence of LP or LM cultures. Adding either LP or LM significantly modulated the microbiota, favoring Streptococcus dominance and reducing overall bacterial diversity compared to non-inoculated cheeses. Raw milk cheeses with added autochthonous cultures exhibited a microbial profile like pasteurized cheeses, suggesting a homogenizing effect on the microbiome. Both cultures effectively reduced microbial load in raw milk cheeses after 20 days, reaching levels comparable to pasteurized cheeses by the end of ripening. Although BA concentrations increased over time, all samples remained within safe limits. Cheeses with LP addition exhibited lower BA levels, suggesting a modulating effect on their biosynthesis. Histamine concentrations were higher in raw milk cheeses with added cultures but remained well below hazardous levels. These findings suggest that incorporating either LP or LM strains is a promising strategy for enhancing the microbial safety and standardization of artisanal goat cheese while preserving its traditional characteristics.

Investigating the release of the bioactive components of novel nutraceuticals combining potentially probiotic Limosilactobacillus fermentum and freeze-dried jabuticaba peel during gastrointestinal digestion for long-term storage

Heloisa Maria Almeida do Nascimento, Thatyane Mariano Rodrigues de Albuquerque, Jaielison Yandro Pereira da Silva, Sônia Paula Alexandrino de Oliveira, Genilton Alves da Silva, Noádia Priscilla Araújo Rodrigues, José Elson Soares Filho 4, Maria Lúcia da Conceição, Mariana Costa Monteiro, Evandro Leite de Souza

DOI: 10.1007/s12602-025-10614-2

This study investigated the release of bioactive components from novel nutraceuticals that combine potentially probiotic Limosilactobacillus fermentum and freeze-dried jabuticaba peel during gastrointestinal digestion for long-term storage. The bioaccessibility of phenolic compounds, antioxidant capacity, cell viability, and physiological status of L. fermentum in two nutraceuticals [mix of potentially probiotic L. fermentum + freeze-dried jabuticaba peel (FJP), termed JM); mix of potentially probiotic L. fermentum + FJP + fructooligosaccharides (FOS), termed JFM)] were assessed when exposed to a standardized simulated gastrointestinal digestion (SGD) during 90 days of storage (4 ± 0.5 °C, 11% relative humidity). JFM showed the highest viable cell counts of L. fermentum (3.76 ± 0.18-5.12 ± 0.12 log CFU/mL) after exposure to SGD during 90 days of storage. JFM presented the largest subpopulation of L. fermentum live cells (29.1 ± 0.66%) and the smallest subpopulation of L. fermentum dead cells (5.8 ± 0.48%) during SGD. The bioaccessibility of anthocyanins in JM and JFM was greater after gastric exposure, while the bioaccessibility of ellagic acid was greater after ileal exposure. The antioxidant capacity of JM and JFM was reduced during SGD and storage, likely due to the degradation of bioactive components. JM and JFM released metabolically active L. fermentum cells and bioaccessible phenolic compounds along the SGD. FOS could have led to JFM performing better overall than JM in the measured variables. The results demonstrate the efficacy of JM and JFM in maintaining their bioactive functionalities during gastrointestinal digestion, allowing them to reach the colonic environment, where they can induce changes in the intestinal microbiota and induce systemic beneficial health effects.

Edible coatings enriched with biocontrol agents for postharvest Management of Penicillium digitatum in lemons

Mariana Andrea Díaz, Karina Felix Dias Fernandes, Evandro Leite de Souza, Julián Rafael Dib

DOI: 10.1002/ps.70085

The worldwide citrus industry faces significant economic losses as a consequence of fungal infections, primarily green mold caused by Penicillium digitatum. In Argentina, a leading lemon producer, these losses pose a substantial challenge to domestic and international markets. Although synthetic fungicides are widely used for green mold control, their effectiveness is hampered by pathogen resistance and growing concerns regarding environmental contamination from chemical residues. These limitations underscore the need for alternative, eco-friendly solutions for postharvest disease control. In this context, the study explores the development of novel edible coatings enriched with biocontrol agents. Specifically, Clavispora lusitaniae (strains AgL21 and 146) and Bacillus velezensis BA3 were combined with alginate and chitosan coatings to evaluate their efficacy in controlling P. digitatum while maintaining fruit quality.

Salmonella enterica in thinly sliced carrots and zucchini survives better at higher temperatures and higher relative humidity conditions

Jerffeson de Lima Tavares, Donald W. Schaffner, Jiin Jung, Carlos Eduardo Barão, Evandro Leite de Souza, Maiara da Costa Lima, Clifton Baldwin, Verônica Ortiz Alvarenga, Geany Targino de Souza Pedrosa, Marciane Magnani 

DOI:10.1016/j.foodres.2025.116998

The global increase in demand for ready-to-eat foods has been accompanied by a concerning rise in salmonellosis outbreaks linked to minimally processed vegetables (MPV). This study evaluated S. enterica survival in minimally processed carrot and zucchini under different combined conditions of temperature (6, 9 and 12 °C) and relative humidity (RH; 75, 85 and 95 %) over 168 h. The permeability and membrane potential of S. enterica cells were analyzed by flow cytometry and cell morphology was evaluated by scanning electron microscopy. Linear models for survival of S. enterica in minimally processed carrot and zucchini were developed. The adjusted R2 coefficient values for survival in the first 24 h were 0.95 for carrot (temperature p ≤2e−16 and RH p = 0.00107) and 0.97 for zucchini (temperature p ≤2e−16 and RH p = 1.72e−05). The adjusted R2 values for the remainder of storage (24 to 168 h) remained high: 0.92 for carrots (temperature p = 2.76e−13 and RH p = 4.15e−10) and 0.91 for zucchini (temperature p = 1.24e−13 and RH p = 2.72e−07). This suggests that the linear model is suitable for describing S. enterica behavior. S. enterica membrane permeability and degree of non-polarization of cell membranes decreased as temperature and RH increased. S. enterica showed significant morphological changes at 168 h of storage. Both temperature and RH affect S. enterica survival in minimally processed carrot and zucchini, with better survival at higher temperature and relativity humidity conditions. The models developed in this study may be useful for managing microbiological risks associated with S. enterica in MPV.

Controlling anthracnose in mango and guava with antifungal coatings of sodium alginate containing hydroalcoholic extracts of fruit by-products fermented by lactic acid bacteria

Talita Silveira Queiroga, Kataryne Árabe Rimá de Oliveira, Karina Felix Dias Fernandes, Jaielison Yandro Pereira da Silva, Jéssica Silva Freire, Jociano da Silva Lins, Francisco Humberto Xavier-Júnior, Josean Fechine Tavares, Evandro Leite de Souza 

DOI: 10.1093/jambio/lxaf219

This study evaluated the efficacy of hydroalcoholic extracts from fruit by-products fermented by lactic acid bacteria (LAB), i.e. a mix of Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263, as active antifungal agents in sodium alginate coatings for the control of anthracnose caused by Colletotrichum spp. in mango cv. Palmer and guava cv. Paluma.

Method and results: The hydroalcoholic extracts of fermented and unfermented acerola, cashew, mango, and grape by-products presented variations in acidity, pH, total soluble solids, ascorbic acid, total phenolic compounds, flavonoids, and antioxidant activity. Sixteen chemical constituents were identified, including sugars and organic molecules, in the hydroalcoholic extracts of fermented and unfermented acerola and mango by-products. Hydroalcoholic extracts of fermented acerola and mango by-products caused the highest mycelial growth inhibition % (%MGI: 19.5%-76.31%) against Colletotrichum spp. using the agar-well diffusion method. The fermented extracts (1 g.L-¹) caused the most promising inhibition (%MGI: 35.00%-82.75%) and were identified as the minimum inhibitory concentration. Coatings containing these extracts delayed the development and severity of anthracnose in mango and guava during 15 days of storage (25°C), with a % anthracnose lesion diameter reduction (%ALDR) between 3.93 and 87.01%.

Conclusion: Hydroalcoholic extracts from fermented acerola and mango by-products exhibited notable antioxidant and antifungal activity. Their incorporation into sodium alginate coatings represents an innovative strategy to control postharvest anthracnose in fruits.

Synbiotic Nutraceutical Mitigates Gestational Diabetes Effects and Cardiovascular Dysfunction in Rat Offspring

Deborah Emanuelle de Albuquerque Lemos, Evandro Leite de Souza, Micaelle Oliveira de Luna Freire, Jaielison Yandro Pereira da Silva, Paulo César Trindade da Costa, Josiane de Campos Cruz, Alisson Macário de Oliveira, João Felipe Mota, José Luiz de Brito Alves

DOI: 10.1002/mnfr.70340

This study investigated the effects of a nutraceutical combining jabuticaba peel (Myrciaria jaboticaba (Vell.) Berg) and Limosilactobacillus fermentum strains on gut microbiota, glycaemic and lipid profiles, and inflammatory markers in rats with gestational diabetes mellitus (GDM), as well as its effects on cardiac autonomic function in the offspring. Pregnant Wistar rats were assigned to control (CTL), GDM, and GDM receiving the nutraceutical (GDM+Nut). The nutraceutical (2 g/kg) or vehicle (PBS) was administered twice daily. After weaning, the offspring were fed laboratory chow until 80 days of age. Nutraceutical administration improved glucose tolerance and reduced serum inflammatory cytokines (TNF-α and IL-6). Beta diversity was increased without altering alpha diversity indices in the GDM+Nut. Nutraceuticals increased the abundance of short-chain fatty acid-producing bacteria. Maternal supplementation protected against autonomic dysfunction in the offspring. Nutraceutical supplementation shows potential as an alternative therapy for the treatment of GDM, while mitigating autonomic dysfunction in offspring.

Deciphering Pigmented Rice Varieties as Sustainable and Unexplored Valuable Sources of Bioactive Components With Health-Related Properties and Technological Applications-A Systematic Review

Adolfo Pinheiro de Oliveira, Thatyane Mariano Rodrigues de Albuquerque, Evandro Leite de Souza

DOI:10.1111/1541-4337.70355

The interest in pigmented grains has grown substantially in recent years. Pigmented rice is crucial for diversifying agricultural production and promoting healthier, more balanced diets. This systematic review updates and discusses literature published between 2019 and 2024 on the bioactive compounds in pigmented rice, their potential health benefits, and technological applications, based on a final sample of 69 articles. Data indicate that pigmented rice varieties have valuable nutritional composition, and their by-products have several constituents that promote health and prevent chronic diseases. The health-related functions of flavonoids, anthocyanins, proanthocyanidins, phenolic acids, resistant starch, dietary fiber, essential fatty acids, and proteins typically found in pigmented rice and its by-products have been confirmed through in vitro and in vivo studies, showcasing anti-inflammatory, anticancer, antioxidant, antidiabetic, and antimicrobial activities. Several varieties of pigmented rice, including black, red, and purple, differ in their nutrient and bioactive compound content and, consequently, in their potential consumer benefits. The presence of these bioactive compounds also improved the technological properties of pigmented rice, such as stability, viscosity, and texture, and favored the development of biodegradable packaging films. These insights support considering pigmented rice varieties as healthy and sustainable food choices for domestic meal preparation and for use by the food industry as functional ingredients to formulate novel, added-value functional food products.

Assessment of the productive and biodegradative non-enzymatic performance of Pleurotus mushrooms grown on agricultural wastes of sugarcane bagasse and banana leaves

Sabrina Alves da Silva, Jhonatan Rafael Zárate-Salazar, Robson Eduardo Pereira Monteiro, Rossana Lucena de Medeiros, Háimyk Andressa Nóbrega de Souza, Bruno de Oliveira Dias, Fillipe de Oliveira Pereira

DOI:10.1007/s11274-025-04373-z 

Selecting a substrate for cultivating mushroom species can provide strategies for utilizing agronomic wastes within a circular economy. In Northeast Brazil, sugarcane and bananas are the most produced crops, generating large amounts of agricultural waste. Thus, this study aimed to evaluate the influence of different sugarcane bagasse (SB) and banana leaf (BL) formulations on the productive and holistic non-enzymatic biodegradative performance of Pleurotus djamor and P. ostreatus. The experiment was randomized with treatments consisting of five formulations: 0% (control), 25%, 50%, 75%, and 100% SB, complemented with BL. The substrates were moistened, bagged, sterilized, and inoculated. They were then subjected to the incubation, fruiting induction, and harvest stages, resulting in three productive cycles. Mycelial growth (mm day⁻1), biological efficiency (%), production rate (% day⁻1), earliness (days), and the biodegrading ratio of the chemical components of the cultivation substrate were assessed. The results were subjected to ANOVA and Tukey’s tests at a 5% significance level (P < 0.05). The 25-SB treatment provided optimal conditions for productive developing P. djamor and showed the highest biodegradative performance. It resulted in high and significant mycelial growth, earliness, and production rate while promoting over 50% lignin biodegrading ratio. P. ostreatus demonstrated high productive performance with over 80% biological efficiency, although no significant differences were observed between treatments. We conclude that agricultural wastes from SB and BL provide essential conditions for cultivating P. djamor and P. ostreatus, with high biodegradative and productive performance, respectively.

Edible mushrooms in Brazil: current state of knowledge, advances and perspectives

Menolli Jr., Nelson, Larissa Trierveiler-Pereira, Diego C. Zied, Fillipe  de  O.  Pereira, Marcelo  A.  Sulzbacher, Elisandro  R.  Dre-chsler-Santos, Diogo H. Costa-Rezende, Daniela Werner, Francisco J. Simões Calaça, Denis A. Zabin, Cristiano C. do

Nascimento, Mariana P. Drewinski

DOI: 10.30550/j.lil/1879 

Brazil is a megadiverse country of continental magnitude and with a great bioeconomic importance worldwide, in addition to having great sociocultural richness and related to the fungal diversity in its ecosystems. The diversity of fungi known for the country is around 13–14 thousand species, including about 2,500 mushroom species. Over 400 species of wild edible mushrooms have already been recorded in Brazil, with more than 80 species having consistent records of occurrence in te country. The consumption of wild edible mushrooms in Brazil is mainly related to traditional knowledge of original people, with around 45 mushroom species already reported as edible by indigenous communities. Despite the great diversity of edible species, the Brazil’s mushroom market is concentrated in the production of exotic species, with emphasis on Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus. Successful studies on the domestication and cultivation of wild isolates in the country involve more than 20 edible species that occur naturally in Brazilian forests, representing a promising future for the insertion of new species into the local market. The production of edible mushrooms in Brazil can help meet some of the Sustainable Development Goals of the United Nations, mainly those related to nutrition and food security, health and well-being, and sustainable production and consumption. Activities focused on scientific outreach, citizen science, science popularization, mycotourism, and foraging of edible mushrooms have gained prominence in recent years, contributing to the popularization of consumption and recognition of mushrooms in the country. Initiatives related to the conservation aspects of wild edible species have advanced in the country with the inclusion of species on the Red List of the International Union for Conservation of Nature. In this context, this article provides an overview of current knowledge, advances, and perspectives on edible mushrooms in Brazil.

Salmonella enterica in thinly sliced carrots and zucchini survives better at higher temperatures and higher relative humidity conditions.

Jerffeson de Lima Tavares, Donald W. Schaffne, Jiin Jung, Carlos Eduardo Barão, Evandro Leite de Souza, Maiara da Costa Lima, Clifton Baldwin, Verônica Ortiz Alvarenga, Geany Targino de Souza Pedrosa, Marciane Magnani

DOI: 10.1016/j.foodres.2025.116998 

The global increase in demand for ready-to-eat foods has been accompanied by a concerning rise in salmonellosis outbreaks linked to minimally processed vegetables (MPV). This study evaluated S. enterica survival in minimally processed carrot and zucchini under different combined conditions of temperature (6, 9 and 12 ◦C) and relative humidity (RH; 75, 85 and 95 %) over 168 h. The permeability and membrane potential of S. enterica cells were analyzed by flow cytometry and cell morphology was evaluated by scanning electron microscopy. Linear models for survival of S. enterica in minimally processed carrot and zucchini were developed. The adjusted R2 coefficient values for survival in the first 24 h were 0.95 for carrot (temperature p ≤2e− 16 and RH p = 0.00107) and 0.97 for zucchini (temperature p ≤2e− 16 and RH p = 1.72e− 05). The adjusted R2 values for the remainder of storage (24 to 168 h) remained high: 0.92 for carrots (temperature p = 2.76e− 13 and RH p = 4.15e− 10) and 0.91 for zucchini (temperature p = 1.24e− 13 and RH p = 2.72e− 07). This suggests that the linear model is suitable for describing S. enterica behavior. S. enterica membrane permeability and degree of non-polarization of cell membranes decreased as temperature and RH increased. S. enterica showed significant morphological changes at 168 h of storage. Both temperature and RH affect S. enterica survival in minimally processed carrot and zucchini, with better survival at higher temperature and relativity humidity conditions. The models developed in this study may be useful for managing microbiological risks associated with S. enterica in MPV.

Controlling anthracnose in mango and guava with antifungal coatings of sodium alginate containing hydroalcoholic extracts of fruit by-products fermented by lactic acid bacteria.

Talita Silveira Queiroga, Kataryne Árabe Rimá de Oliveira, Karina Felix Dias Fernandes, Jaielison Yandro Pereira da Silva, Jéssica Silva Freire, Jociano da Silva Lins, Francisco Humberto Xavier-Júnior, Josean Fechine Tavares, Evandro Leite de Souza.

DOI: 10.1093/jambio/lxaf219

Aim: This study evaluated the efficacy of hydroalcoholic extracts from fruit by-products fermented by lactic acid bacteria (LAB), i.e. a mix of Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263, as active antifungal agents in sodium alginate coatings for the control of anthracnose caused by Colletotrichum spp. in mango cv. Palmer and guava cv. Paluma. Method and results: The hydroalcoholic extracts of fermented and unfermented acerola, cashew, mango, and grape by-products presented variations in acidity, pH, total soluble solids, ascorbic acid, total phenolic compounds, flavonoids, and antioxidant activity. Sixteen chemical constituents were identified, including sugars and organic molecules, in the hydroalcoholic extracts of fermented and unfermented acerola and mango by-products. Hydroalcoholic extracts of fermented acerola and mango by-products caused the highest mycelial growth inhibition % (%MGI: 19.5%-76.31%) against Colletotrichum spp. using the agar-well diffusion method. The fermented extracts (1 g.L-¹) caused the most promising inhibition (%MGI: 35.00%-82.75%) and were identified as the minimum inhibitory concentration. Coatings containing these extracts delayed the development and severity of anthracnose in mango and guava during 15 days of storage (25°C), with a % anthracnose lesion diameter reduction (%ALDR) between 3.93 and 87.01%. Conclusion: Hydroalcoholic extracts from fermented acerola and mango by-products exhibited notable antioxidant and antifungal activity. Their incorporation into sodium alginate coatings represents an innovative strategy to control postharvest anthracnose in fruits.

Synbiotic Nutraceutical Mitigates Gestational Diabetes Effects and Cardiovascular Dysfunction in Rat Offspring.

Deborah Emanuelle de Albuquerque Lemos, Evandro Leite de Souza, Micaelle Oliveira de Luna Freire, Jaielison Yandro Pereira da Silva, Paulo César Trindade da Costa, Josiane de Campos Cruz, Alisson Macário de Oliveira, João Felipe Mota, José Luiz de Brito Alves

DOI: 10.1002/mnfr.70340 

This study investigated the effects of a nutraceutical combining jabuticaba peel (Myrciaria jaboticaba (Vell.) Berg) and Limosilactobacillus fermentum strains on gut microbiota, glycaemic and lipid profiles, and inflammatory markers in rats with gestational diabetes mellitus (GDM), as well as its effects on cardiac autonomic function in the offspring. Pregnant Wistar rats were assigned to control (CTL), GDM, and GDM receiving the nutraceutical (GDM+Nut). The nutraceutical (2 g/kg) or vehicle (PBS) was administered twice daily. After weaning, the offspring were fed laboratory chow until 80 days of age. Nutraceutical administration improved glucose tolerance and reduced serum inflammatory cytokines (TNF-α and IL-6). Beta diversity was increased without altering alpha diversity indices in the GDM+Nut. Nutraceuticals increased the abundance of short-chain fatty acid-producing bacteria. Maternal supplementation protected against autonomic dysfunction in the offspring. Nutraceutical supplementation shows potential as an alternative therapy for the treatment of GDM, while mitigating autonomic dysfunction in offspring.

Deciphering Pigmented Rice Varieties as Sustainable and Unexplored Valuable Sources of Bioactive Components With Health-Related Properties and Technological Applications-A Systematic Review.

Adolfo Pinheiro de Oliveira, Thatyane Mariano Rodrigues de Albuquerque, Evandro Leite de Souza

DOI:10.1111/1541-4337.70355

The interest in pigmented grains has grown substantially in recent years. Pigmented rice is crucial for diversifying agricultural production and promoting healthier, more balanced diets. This systematic review updates and discusses literature published between 2019 and 2024 on the bioactive compounds in pigmented rice, their potential health benefits, and technological applications, based on a final sample of 69 articles. Data indicate that pigmented rice varieties have valuable nutritional composition, and their by-products have several constituents that promote health and prevent chronic diseases. The health-related functions of flavonoids, anthocyanins, proanthocyanidins, phenolic acids, resistant starch, dietary fiber, essential fatty acids, and proteins typically found in pigmented rice and its by-products have been confirmed through in vitro and in vivo studies, showcasing anti-inflammatory, anticancer, antioxidant, antidiabetic, and antimicrobial activities. Several varieties of pigmented rice, including black, red, and purple, differ in their nutrient and bioactive compound content and, consequently, in their potential consumer benefits. The presence of these bioactive compounds also improved the technological properties of pigmented rice, such as stability, viscosity, and texture, and favored the development of biodegradable packaging films. These insights support considering pigmented rice varieties as healthy and sustainable food choices for domestic meal preparation and for use by the food industry as functional ingredients to formulate novel, added-value functional food products.

Assessment of the productive and biodegradative non-enzymatic performance of Pleurotus mushrooms grown on agricultural wastes of sugarcane bagasse and banana leaves.

Sabrina Alves da Silva, Jhonatan Rafael Zárate-Salazar, Robson Eduardo Pereira Monteiro, Rossana Lucena de Medeiros, Háimyk Andressa Nóbrega de Souza, Bruno de Oliveira Dias, Fillipe de Oliveira Pereira

DOI: 10.1007/s11274-025-04373-z

Selecting a substrate for cultivating mushroom species can provide strategies for utilizing agronomic wastes within a circular economy. In Northeast Brazil, sugarcane and bananas are the most produced crops, generating large amounts of agricultural waste. Thus, this study aimed to evaluate the influence of different sugarcane bagasse (SB) and banana leaf (BL) formulations on the productive and holistic non-enzymatic biodegradative performance of Pleurotus djamor and P. ostreatus. The experiment was randomized with treatments consisting of five formulations: 0% (control), 25%, 50%, 75%, and 100% SB, complemented with BL. The substrates were moistened, bagged, sterilized, and inoculated. They were then subjected to the incubation, fruiting induction, and harvest stages, resulting in three productive cycles. Mycelial growth (mm day⁻1), biological efficiency (%), production rate (% day⁻1), earliness (days), and the biodegrading ratio of the chemical components of the cultivation substrate were assessed. The results were subjected to ANOVA and Tukey’s tests at a 5% significance level (P < 0.05). The 25-SB treatment provided optimal conditions for productive developing P. djamor and showed the highest biodegradative performance. It resulted in high and significant mycelial growth, earliness, and production rate while promoting over 50% lignin biodegrading ratio. P. ostreatus demonstrated high productive performance with over 80% biological efficiency, although no significant differences were observed between treatments. We conclude that agricultural wastes from SB and BL provide essential conditions for cultivating P. djamor and P. ostreatus, with high biodegradative and productive performance, respectively.

Cogumelos comestíveis no Brasil: estado atual do conhecimento, avanços e perspectivas.

Menolli Jr, Nelson, Larissa Trierveiler-Pereira, Diego C. Zied, Fillipe de O. Pereira, Marcelo A. Sulzbacher, Elisandro R. Dre-chsler-Santos, Diogo H. Costa-Rezende, Daniela Werner, Francisco J. Simões Calaça, Denis A. Zabin, Cristiano C. do Nascimento, Mariana P. Drewinski

DOI: 10.30550/j.lil/1879

O Brasil é um país megadiverso, de magnitude continental e de grande importância bioeconômica no cenário mundial, além de possuir grande riqueza sociocultural e relacionada à diversidade de fungos em seus ecossistemas. A diversidade de fungos conhecida para o país está em torno de 13–14 mil espécies, incluindo cerca de 2.500 espécies de cogumelos. Mais de 400 espécies de cogumelos comestíveis silvestres já foram registradas para o Brasil, sendo mais de 80 espécies com registros consistentes de ocorrência no país. O consumo de cogumelos comestíveis silvestres no Brasil está associado principalmente ao conhecimento tradicional dos povos originários, sendo cerca de 45 espécies de cogumelos já registradas como comestíveis por comunidades indígenas. Apesar da grande diversidade de espécies comestíveis, o mercado nacional de cogumelos está concentrado na produção de espécies exóticas, com destaque para Agaricus bisporus, Lentinula edodes e Pleurotus ostreatus. Estudos bem-sucedidos de domesticação e cultivo de isolados silvestres no país envolvem mais de 20 espécies comestíveis que ocorrem naturalmente nas matas brasileiras, representando um futuro promissor para a inserção de novas espécies no mercado nacional. A produção de cogumelos comestíveis no Brasil pode auxiliar no cumprimento de alguns dos Objetivos de Desenvolvimento Sustentável da Organização das Nações Unidas, principalmente aqueles relacionados à nutrição e segurança alimentar, saúde e bem-estar e produção e consumo sustentáveis.Atividades de divulgação científica,ciência cidadã,popularização da ciência, micoturismo e extrativismo de cogumelos comestíveis têm ganhado destaque nos últimos anos, contribuindo para a popularização do consumo e reconhecimento de cogumelos no país. Iniciativas relacionadas aos aspectos de conservação de espécies comestíveis silvestres têm avançado no país com a inclusão de espécies na Lista Vermelha da União Internacional para a Conservação da Natureza. Neste contexto, este artigo traz um recorte do conhecimento atual, dos avanços e das perspectivas sobre os cogumelos comestíveis no Brasil.

STUDY ON THE EFFECT OF CARVACROL IN ASSOCIATION WITH AMPHOTERICIN B AGAINST Cryptococcus neoformans STRAIN (LM-22).

Rafaela de Oliveira Nobrega, Anna Paula de Castro Teixeira, Francisco Patricio de Andrade Júnior, Edeltrudes de Oliveira Lima

DOI: 10.20873/2025_jul_19833

Cryptococcus  neoformans  is  an  encapsulated  yeast,  etiologic  agent  of cryptococcosis,  an  opportunistic  and  systemic  fungal  infection,  frequent  in patients  with  compromised  immune  systems.  Considering  the  therapeutic potential  of  phytochemicals  derived  from  natural  products  they  are  fast becoming promising alternatives for both fungal infections and other diseases. The  carvacrol  is  found  in  various  plant  essential  oils,  and  possesses  many antifungal and  antibacterial  activities.  The  study aims  to evaluate the in vitro antifungal  activity  of  the  carvacrol  against  Cryptococcus  neoformans.  An antifungal activity investigation was performed using microbial death kinetics, and studying carvacrol in associations with amphotericin B and their effect on C. neoformans micromorphology. Using Time to Kill method, our results show that carvacrol  inhibits  fungal  growth,  and presents indifferent effects when in association  with  amphotericin  B.  Carvacrol  also promoted  capsule thickness reductions.  Under  the  conditions  evaluated  carvacrol  presented  antifungal activity against C. neoformans strain LM-22.

Pleurotus Mushrooms in Nutrition and Health: Clinical and Preclinical Insights for Nutraceutical Development.

Patrícia Lima Araújo, Ediana da Silva Araújo, Erika Mayra de Almeida Barreto, José Luiz de Brito Alves, Kamylla Mylena Souza, Micaelle Oliveira de Luna Freire, Rayane Maria Pessoa de Souza, Fillipe de Oliveira Pereira

DOI: 10.1111/1541-4337.70279

Pleurotus mushrooms are fungi widely consumed due to their high nutritional value and potential applications as nutraceuticals. Their sustainable cultivation and rich composition of bioactive compounds provide significant health benefits. This review examines the scientific evidence regarding the safety, efficacy, and nutraceutical potential of Pleurotus species, focusing on their effects on various human diseases. The review incorporates findings from preclinical, clinical studies, and nutraceutical formulations related to innovative Pleurotus-based products. Preclinical studies have shown that Pleurotus species can reduce inflammatory markers, modulate gut microbiota, and improve lipid and glucose metabolism. As a result, these mushrooms exhibit potential hypoglycemic, anti-obesity, hepatoprotective, neuroprotective, anti-atherogenic, and anticancer properties, along with possible benefits for preventing Alzheimer’s disease. Clinical trials suggest that consuming Pleurotus has a positive effect on metabolic parameters in healthy individuals and patients with chronic conditions. However, the variability among studies and the absence of standardized nutraceutical formulations hinder definitive conclusions about their therapeutic efficacy. Despite the promising potential of Pleurotus mushrooms in the nutraceutical sector, future research should focus on developing standardized formulations, optimizing bioavailability, expanding clinical trials, exploring the diversity of native species, and uncovering the underlying mechanisms of action to establish their practical application as nutraceuticals.

Novel antimicrobial and antiangiogenic properties from riparin-type benzamides: synthesis, biological evaluation, and docking analysis. 

Gabriela R. de Sousa, Janaracy L. da Costa Marinho, Pedro Thiago R. de Figueiredo, João Pedro A. Torres, Laísa V. Cordeiro, Luciana Scotti, Edeltrudes O. Lima, Bruna Braga Dantas, Fillipe de Oliveira Pereira, Jailton De Souza-Ferrari, José Maria Barbosa-Filho

DOI:10.1007/s00210-025-04730-7 

The global increase in antimicrobial resistance, along with the role of pathological angiogenesis in infection-associated inflammation, underscores the need for multifunctional therapeutic agents. In this study, a series of riparin-type benzamides were synthesized using efficient and accessible synthetic methods, yielding structurally diverse compounds, and their antimicrobial and antiangiogenic potential was evaluated. Among these, riparin III (3c) exhibited the most potent and broad-spectrum antimicrobial activity, effectively inhibiting Gram-positive and Gram-negative bacterial strains as well as Candida spp. Furthermore, riparin I (3a), riparin II (3b), and riparin III (3c) exhibited antiangiogenic activity, suggesting their potential therapeutic relevance in infection-associated inflammation. Toxicity assessments indicated low cytotoxicity at the effective concentrations, supporting the safety profile of the compounds. Molecular docking analysis corroborated the in vitro findings, revealing strong interactions between riparin I (3a) and fungal cell wall targets, as well as between riparin III (3c) and key bacterial enzymes involved in resistance and replication. Overall, these results highlight riparin I (3a), riparin II (3b), and riparin III (3c) as promising multifunctional candidates for the development of antimicrobial agents capable of addressing the current challenges in infection control, including antimicrobial resistance and inflammation-associated pathologies.

Development and functional stability of a next-generation nutraceutical combining Pleurotus djamor mushroom and Limosilactobacillus fermentum strains.

Kamylla Mylena Souza Barbosa, Evandro Leite de Souza, Francinalva Dantas de Medeiros, Giuliane Moura Andrade, Jaielison Yandro Pereira da Silva, José Luiz de Brito Alves, Jhonatan Rafael Zárate-Salazar, Marcos dos Santos Lima, Maria Elieidy Gomes de Oliveira, Natália Sufiatti Holanda Cavalcanti, Maiara da Costa Lima, Yasmim Fonseca Simplicio da Silva, Fillipe de Oliveira Pereira

DOI: 10.1016/j.fbp.2025.11.026 

This study reports the development and evaluation of the viability and functional stability of potentially symbiotic nutraceuticals formulated with the edible mushroom Pleurotus djamor and probiotic Limosilactobacillus fermentum (139, 263, and 296). The nutraceutical (NPD) was produced by combining P. djamor powder with L. fermentum strains and freeze-drying, and its physicochemical properties, probiotic viability, and stability were assessed under storage and simulated gastrointestinal digestion. NPD presented high protein and fiber content, low fat, and phenolic compounds (epigallocatechin gallate, catechin, and epicatechin) associated with antioxidant activity. After 90 days, the viable cell counts decreased significantly at both 4 ± 0.5 °C (NPD4) and 25 ± 0.5 °C (NPD25) (p ≤ 0.05). However, flow cytometry revealed that NPD4 better preserved viable cells and phenolic stability compared to NPD25 (p ≤ 0.05). A substantial fraction of cells remained metabolically active but non-culturable under both storage conditions, with higher values consistently observed in NPD4. Although P. djamor powder provided partial protection, simulated gastrointestinal digestion still caused a progressive decline in cell counts, probiotic viability and antioxidant activity. This is the first study to combine P. djamor with a multi-strain L. fermentum. The nutraceutical strategy described herein represents a promising approach for developing next-generation nutraceuticals targeting gut health.

Phytochemical and pharmacological aspects of the genus Chamaecrista: a systematic review.

Domitila Villalba, Melissa Escobar, Cinthia Cazal Martínez, Javier E. Barúa,  Fillipe De Oliveira Pereira, Juliana Moura-Mendes

DOI:  10.7324/JAPS.2025.241066

The genus Chamaecrista, belonging to the family Fabaceae, is mainly distributed in the American continent and is of great interest because of its extensive use in traditional medicine. This article presents a systematic review of the pharmacological and chemical properties of the Chamaecrista. Articles published between 2004 and 2023 were grouped through searches in the following databases: PubMed, SciELO, Scopus, Science Direct, Web of Science, and Google Scholar. Articles were included based on the selection and classification of titles and abstracts if they indicated the country of origin of the studies, plant species studied, compounds identified, and associated pharmacological activities. The results showed that most of the studies conducted in Brazil represented 35% of the published works. Moreover, Chamaecrista nictitans and Chamaecrista duckeana have been the most studied for their pharmacological potential, especially for their antioxidant activity. In addition, flavonoids were the leading group described, obtained from different extracts of various parts of the plant, with leaves being the most utilized. Despite promising advances in understanding the chemical composition and pharmacological properties of the genus Chamaecrista, this review highlights that no preclinical in vivo and clinical studies have been reported. This study underscores the importance of continuing research to identify and isolate new bioactive molecules from this genus, aiming to elucidate their pharmacological functions and potential therapeutic applications.

One-Health Approach to Managing Aflatoxin-Producing Using Spent Mushroom Substrate of spp.

Wesley Morais de Araújo, Emanuel Pereira Silva, Gessymara Cainã Sales da Silva, Lívia Soares de França Silva, Francinalva Dantas de Medeiros, José Eduardo Gonçalves, Bruna Braga Dantas, Kelvyn Kennedy de Figueiredo Silva, Anauara Lima E Silva, Josean Fechine Tavares, Jhonatan Rafael Zárate-Salazar, Juliana Moura-Mendes, Fillipe de Oliveira Pereira

DOI: 10.1021/acsomega.5c00858

Food loss represents a critical global concern. Aspergillus flavus is central to this issue, contaminating maize crops with aflatoxins. The agricultural fungicide carboxin presents significant challenges due to the rise of resistant fungal strains. This study investigates the antifungal efficacy of spent mushroom substrates from Pleurotus ostreatus (SPoS) and P. djamor (SPdS) cultivated on banana leaves, combined with carboxin (CBX), against A. flavus strains isolated from maize. The SPoS and SPdS extracts showed a low biogenic amine content and a global profile (1H NMR) of volatile oils, unsaturated fatty acids, polysaccharides, oligosaccharides, tannins, and flavonoids. A. flavus strains were not susceptible to amphotericin B and itraconazole. SGPo and SGPd exhibited fungicidal activity against all strains tested, with MIC values ranging from 1024 to 2048 mg/L. CBX demonstrated fungicidal activity with MIC values ranging from 32 to 512 mg/L. No antagonism between CBX+SPdS and CBX+SPoS was observed. SPoS and SPdS showed significant inhibition of conidial germination and mycelial growth, but CBX+SPdS and CBX+SPoS were more potent than individual agents (p < 0.05). SPoS and SPdS reduced conidial germination and mycelial growth by more than 70%. The study also assessed the irritation potential of these agents using the HET-CAM model, classifying them as moderate irritants. These findings support that SPdS and SPoS can potentially reduce the required dosages and frequency of application of CBX, leading to more sustainable antifungal treatments with minimized environmental and resistance risks, which aligns with a one-health approach.

Nutritional composition, toxicity, and the effects of Parkia biglobosa on the metabolic, somatic and oxidative parameters related to liver and kidney function of obese rats. Journal of Functional Foods.

Mirela Gouveia-Nhanca, Maria Luiza Rolim Bezerra, Yuri Mangueira do Nascimento, Evandro Ferreira da Silva, Josean Fechine Tavares, Gonçalo Mendes da Conceição, Rubens Teixeira de Queiroz, Luis António Tavares, Adriano Francisco Alves, Jailane de Souza Aquino 

DOI:10.1016/j.jff.2024.106479 

The pathophysiology of obesity is associated with somatic, metabolic and oxidative changes which can also compromise renal and hepatic function. Treatment of this condition involves dietary intervention, including incorporating foods rich in bioactive compounds such as Parkia biglobosa. This legume is rich in fibres and phenolic compounds such as 3,4-dihydroxybenzoic acid, catechin, and myricetin that can play a significant role in the adjuvant treatment of obesity. Thus, we evaluated the nutritional composition, toxicity, and the effects of Parkia biglobosa on metabolic, somatic and oxidative parameters, as well as the liver and kidney function of obese rats.

Impact of different prebiotics on ultrasound-treated nopal cladode (Opuntia dillenii) beverages.

Januse Míllia Dantas de Araújo, Marciane Magnani, Whyara Karoline Almeida da Costa, Carlos Eduardo Barão, Tatiana Colombo Pimentel, Marcos dos Santos Lima, Maria Elieidy Gomes de Oliveira, Angela Maria Tribuzy de Magalhães Cordeiro, Jailane de Souza Aquino 

DOI: 10.1016/j.foodchem.2024.141721

puntia dillenii cladodes are rich in bioactive compounds such as phenolics, vitamins and fibres. This study aimed to evaluate the impact of different prebiotics on the bioactive compounds’ stability, bioacessibility, bioactivity and sensorial characteristics of ultrasound-treated nopal cladode (Opuntia dillenii) beverages. Five formulations were prepared: untreated beverage (BC); ultrasound-treated beverage (BU); and ultrasound-treated beverage with inulin (BIU) or fructo-oligosaccharides (BFU) or polydextrose (BPU). The addition of prebiotics increased dietary fibre in beverages (10.44–20.70 %). BPU presented higher concentrations and stability of bioactive compounds, such as phenolics and ascorbic acid; in addition to greater antioxidant activity (FRAP) and maintained the inhibition of the α-glucosidase enzyme. BFU showed higher bioaccessibility of phenolic compounds and maintained inhibition of the α-amylase enzyme. The addition of prebiotics minimized undesirable sensory characteristics in the beverages. These findings suggest that adding prebiotics to nopal beverages may maintain functionality and increase the acceptability of these products during storage.

In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese ratsShort tittle: effects of facheiro mucilage in obese rats.

Fabrícia de Souza Ferreira, Hassler Clementino Cavalcante, Larissa Maria Gomes Dutra, Adriano Francisco Alves, Stephson Andriola Almeida Gonçalves, T. C. Pimentel, C. Barão, Marcos dos Santos Lima, Alana Natalícia Vasconcelos de Araújo, Januse Míllia Dantas de Araújo, K. S. Batista, Jailane de Souza Aquino, Angela Maria Tribuzy de Magalhães Cordeiro

DOI: 10.1016/j.jff.2024.106575

We evaluated the in vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats. First, in vitro antidiabetic activity was analysed through the inhibition of α-amylase and α-glucosidase. Then, the acute toxicity of mucilage was evaluated in Wistar rats for 14 days. After, an experiment to evaluate the effects of mucilage on obesity was conducted with 30 male Wistar rats randomised into three groups: healthy (HG); obese (OG); and obese treated with facheiro mucilage (FOG) by gavage (200 mg/kg). The HG received commercial diet and the OG and FOG received a cafeteria diet for 12 weeks. Facheiro mucilage showed antidiabetic activity in vitro by reducing the α-amylase (64.15 ± 3.86 %) and α-glucosidase activity (86.43 ± 1.09 %), without signs of acute toxicity in vivo. Mucilage treatment reduced insulinemia (OG = 29.70 ± 4.52 µIU/mL vs. FOG = 14.04 ± 5.08 µIU/mL, p = 0.01, effect size: 3.25), homeostasis assessment model (OG = 0.99 ± 0.12, FOG = 0.76 ± 0.18, p = 0.03, effect size: 1.50), uric acid (OG = 6.65 ± 1.63 mg/dL vs. FOG = 4.07 ± 0.65 mg/dL, p = 0.0001, effect size: 2.07), creatinine (OG = 0.90 ± 0.08 mg/dL vs. FOG = 1.05 ± 0.15 mg/dL, p = 0.0001, effect size: 1.24), alanine transaminase (OG = 29.70 ± 4.52 IU/L vs. FOG = 14.05 ± 5.08 IU/L, p = 0.0001, effect size: 3.25), aspartate transaminase (OG = 58.55 ± 7.10 IU/L vs. FOG = 22.35 ± 3.61 IU/L, p = 0.0001, effect size: 6.42) and the hepatic steatosis in the FOG compared to the OG. Mucilage intake increased the total concentrations of organic acids and sugars in renal and hepatic tissues of the FOG when compared to the OG. However, overall, it did not alter the murinometric parameters. Our results suggest that facheiro mucilage had antidiabetic activity in vitro, which corroborated the improvement of insulinemia and insulin resistance in FOG rats; in addition, it showed renoprotective and hepatoprotective effects, highlighting a reversal in the severity of hepatic steatosis in obese rats.

Maternal consumption of nut oil (Bertholletia excelsa): Evidence of anxiolytic-like behavior and reduction in brain lipid peroxidation on the progeny of rats

Larissa Maria Gomes Dutra, Maria Elizângela Ferreira Alves, Thales Daniel Oliveira de Lima e Silva, Januse Millia Dantas de Araújo, Maciel da Costa Alves Silva , Diego Elias Pereira, Rita de Cássia de Araújo Bidô, Juliano Carlo Rufino Freitas, Vanessa Bordin Viera, Jailane Aquino de Souza, Juliana Késsia Barbosa Soares

DOI: 10.1016/j.brainres.2025.14950

Introduction: Maternal nutrition plays a crucial role in the development of offspring, influencing both biochemical and behavioral parameters. Brazil nut oil, rich in essential fatty acids and antioxidant bioactive compounds, may provide metabolic, neuroprotective, and anxiolytic benefits to the offspring when offered during the critical period of development.

Objective: Investigate the impact of maternal consumption of crude or refined Brazil nut oil during pregnancy and lactation on anxiety-like behavior and brain lipid peroxidation in rat offspring.

Methodology: Each group were compound by male (M) and female (F) puppies. The groups formed were: Controls (CG-M and CG-F) -treated with distilled water; Crude oil (CO-M and CO-F) receiving 3000 mg/kg of body weight of Brazil nut crude oil, and Refined oil (RO-M and RO-F) – treated with 3000 mg/kg of body weight of refined Brazil oil. The dams were treated during pregnancy and lactation. Anxiety-like behavior was observed in the offspring during adolescence using: elevated plus maze (EPM), open field (OF) and light-dark box (LDB). Malondialdehyde (MDA) levels were measured in the pups’ brain tissue.

Results: RO-M/RO-F entered and spent more into the open arms and realized more head dipping CO-M/CO-F and CO-F/RO-F presented increased locomotion and less grooming in the OF; RO-M and RO-F realized more rearing compared to controls groups and CO-M and CO-F compared to all groups. All groups treated with crude and refinated oil spent more time in the light area and realized more transitions in the LDB. Cerebral MDA were decreased in all experimental groups compared to controls groups.

Conclusion: Maternal comsuption of Brazil nut oil induced anxiolitic-like behavior and reduced brain lipid peroxidation of the male and female offspring in rats.

Impacto do jejum intermitente pré-concepcional na ontogênese reflexa e no desenvolvimento físico e somático da prole de ratos Wistar.

Alana Natalícia Vasconcelos de Araújo, Larissa Maria Gomes Dutra, Bruno Silva Dantas, Adriano Francisco Alves, Stephenson Andriola Almeida Gonçalves, Angela Maria Tribuzy de Magalhães Cordeiro, Juliana Késsia Barbosa Soares, Jailane de Souza Aquino

 DOI: 10.1016/j.brainres.2025.149510

Background: Intermittent fasting (IF) has health benefits; however, little is known about its effects on the offspring when practiced by dams in the preconception period. The aim of the study was to evaluate the impact of IF during the preconception period on physical and somatic parameters, as well as on the reflex ontogenesis of Wistar rat offspring.

Methods: The female rats were randomized into two groups: control (CG) and intermittent fasting group (IF) submitted to a feed restriction of 16 h for four weeks during the preconception period. Reproductive performance, body weight, and energy intake were evaluated in dams. Reflex ontogeny, physical and somatic development, brain fatty acids and the brain histology were evaluated in the offspring.

Results: IF did not change the reproductive performance or weight of dams during the preconception period. An acceleration of vibrissae placing and negative geotaxis parameters of reflex ontogenesis was observed, as well as anticipation of the following physical development indicators: ear unfolding, an eruption of upper incisor teeth, and inferior teeth in the IF offspring. Although the nervous tissue did not present histological changes, the content of some brain fatty acids was found in greater amounts in the IF offspring such as dihomo-γ-linolenic acid (0.66 ± 0.01 %) and eicotrienoic acid (0.58 ± 0.03 %).

Conclusion: IF during preconception did not change the body weight or reproductive performance of the dams, and promoted beneficial effects on the neurodevelopment of the offspring in the early life.

Different intermittent fasting regimens decrease anxious and depressive-like behaviour, but show damage to brain tissues in healthy Wistar rats

Naís Lira Soares, Hassler Clementino Cavalcante, Soniellson de Lima Ferreira, Fernanda Beatriz Lima do Nascimento, Isabelle Karoline Carvalho Costa, Thaís Bayma Barbosa Rolim, Marcos Dos Santos Lima, Adriano Francisco Alves, Kamila Sabino Batista, Jailane de Souza Aquino 

 DOI: 10.1080/1028415X.2025.2533967

Background: Different intermittent fasting (IF) regimens have been used in healthy individuals to improve body composition; however, other potential benefits or harm, such as modulation of behaviours, are little studied in healthy populations. The present study aimed to evaluate the influence of different IF regimens on parameters related to the behavioural and cerebral aspects in healthy rats and compare them to caloric restriction.

Methods: Adult Wistar rats were randomised into five groups: control (CON); caloric restriction (CR); time-restricted feeding (TRF); alternate-day fasting (ADF); and alternate-day modified fasting (ADMF), and then evaluated during four weeks.

Results: The ADF and ADMF groups had the lowest weight over the weeks and decreased depressive-like behaviour, while TRF decreased anxiety-like behaviour. However, all experimental groups presented some damage in the brain tissues (central cortex and hippocampus), and ADF had the largest area of ⁣⁣NF-κB marking.

Conclusion: Although all groups submitted to IF showed benefits in behaviour, they also presented damage to brain tissues (gemistocystic astrocytes, and ischemic neuronal cell bodies).

Does parental diet alter the neurobehaviour and the reflex and somatic parameters of the offspring?

Rafael Oliveira Pinheiro, Juliana Késsia Barbosa Soares, Anne Caroline Alves Vieira, Maria Luiza Rolim Bezerra, Geyse Araújo Costa, Mariany Bernardino da Silva Barbalho, Artur D’ Angelo da Silva Andrade, Angela Maria Tribuzy de Magalhães Cordeiro, Adriano Francisco Alves, Alana Natalícia Vasconcelos de Araújo, Jailane de Souza Aquino 

 DOI: 10.1016/j.brainres.2025.149860

Background: The early-life nutritional environment plays crucial role in shaping offspring development. While most studies have assessed maternal or paternal high-fat diet (HFD) exposure independently, the combined impact of both remains poorly understood across developmental, metabolic, and behavioural parameters. This study evaluated the effects of maternal and paternal HFD consumption on offspring metabolism, neurodevelopment, and anxiety-like behaviour.

Methods: Male and female Wistar rats were randomised into four dietary groups (n = 10/group): NF/NF (both sexes received a normal-fat diet, NFD), HF/NF (only males received an HFD), NF/HF (only females received an HFD), and HF/HF (both sexes received an HFD). Offspring were assessed for physical growth, somatic maturation, reflex ontogeny (postnatal days 0-21), anxiety-like behaviours (open field and elevated plus maze tests), lipid profile, brain fatty acid composition, and prefrontal cortex histology.

Results: NF/HF and HF/HF groups showed delayed somatic growth and altered physical development. Reflex maturation was impaired in HF/NF and NF/HF offspring. Anxiety-like behaviours were more evident in NF/HF and HF/HF groups. The HF/HF group exhibited elevated total cholesterol (439.28 ± 71.88 mg/dL), higher LDL (151.11 ± 28.72 mg/dL), reduced HDL (36.84 ± 18.93 mg/dL), reduced neuronal cell body size, and lower levels of brain polyunsaturated fatty acids.

Conclusion: Combined maternal and paternal HFD intake exerts cumulative adverse effects on offspring, impairing physical development, reflex maturation, behaviour, and brain lipid composition. These findings provide novel evidence that parental nutritional status prior to conception jointly influences offspring neurobiological and metabolic trajectories, underscoring the importance of considering both maternal and paternal diets in early-life health strategies.

Consumption of goat yogurt enriched with Cereus jamacaru DC. modulates the gut-brain axis inducing anxiolytic-like behavior in young rats

Larissa Maria Gomes Dutra, Jailane de Souza Aquino, Maria Elizângela Ferreira Alves, Renally de Lima Moura, Yuri Mangueira do Nascimento, Marcelo Sobral da Silva, Josean Fechine Tavares, Fábio Anderson Pereira da Silva, Valquíria Cardoso da Silva Ferreira, Wydemberg Jose de Araújo, Juliano Carlo Rufino Freitas, Vanessa Bordin Viera 8, Diego Elias Pereira, Juliana Késsia Barbosa Soares

 DOI: 10.1016/j.physbeh.2025.115063

The aim of this study was to evaluate the effect of consuming goat’s milk yogurt supplemented with mandacaru cladode flour on anxiety-related behavior and the gut-brain axis in young rats. The animals were divided into three groups: Control Group (CG), which received distilled water; the Goat’s Milk Yoghurt Group (YG), treated with pure goat’s milk yoghurt; and a Goat’s Milk Yoghurt with Mandacaru Group (YGM), which received goat’s milk yoghurt supplemented with 7 % mandacaru cladode flour. Anxiety-like behavior was assessed using the Elevated Plus Maze, Open Field, and Light-Dark Box tests. Levels of malondialdehyde, carbonyl compounds, and the brain fatty acid profile were also evaluated. The YG and YGM groups spent more time in the open arms and in the center of the maze compared to the CG, with the YGM group showing a greater time and number of entries into the open arms than the YG group. Both groups exhibited increased locomotion, rearing behavior, and spent less time in the corners of the open field in comparison to the CG, with the YGM group showing greater ambulation than the YG group. In the light-dark box, both YG and YGM groups remained longer in the light compartment than the CG. A reduction in brain malondialdehyde and carbonyl compound levels was observed in both the YG and YGM groups. The YGM group also showed positive modulation of the fecal microbiota, with increased microbial diversity and richness, particularly an increase in the beneficial genera Blautia and Fusicatenibacter, compared to both the YG and CG groups. In conclusion, the consumption of goat’s milk yogurt enriched with mandacaru cladode flour modulated the gut-brain axis in young rats, contributing to the induction of anxiolytic-like behavior.

Analysis of heart rate as a predictor of changes in heart rate variability in children

Paulo César Trindade Costa, Adélia da Costa Pereira de Arruda Neta, Vinícius José Baccin Martins, José Luiz de Brito Alves

DOI: 10.1590/1984-0462/2025/43/2024045

Abstract in English, Portuguese

Objective: To evaluate the predictive validity and cut-off point of heart rate and blood pressure on heart rate variability (HRV) changes in children with and without obesity.

Methods: This study included 125 children, of whom 41 were normal weight and 84 were obese. Anthropometry, blood pressure, heart rate, and HRV were measured using an electronic scale and stadiometer, a sphygmomanometer, and HRV monitor. In addition, the receiver operating characteristic (ROC) curve was obtained by statistical analysis of the data.

Results: Heart rate proved to be a good predictor for changes in the square root of the mean of the square of the differences between consecutive NN intervals (RMSSD) parameter in students of both sexes for the normal-weight group (ROC 0.89; 95%CI 0.77-1.00) and obesity (ROC 0.90; 95%CI 0.83-0.97). In addition, the heart rate cut-off point for alterations in the RMSSD parameter for normal-weight boys was 93 bpm (sensitivity 100.00% and specificity 87.50%) and for boys with obesity, the established cut-off point was 91 bpm (sensitivity 94.74% and specificity 63.64%). Heart rate also proved to be a good predictor considering low-frequency/high-frequency ratio (LF/HF) and standard deviation of long-term continuous NN intervals /standard deviation of the instantaneous variability of continuous NN intervals in the Poincaré graph ratio (SD2/SD1). Systolic and diastolic blood pressures were good predictors in more specific stratifications and, therefore, can be used in some cases.

Conclusions: The predictive validity of heart rate was shown to be at a good level, with high sensitivity and acceptable specificity for the cut-off points according to the different analyses stratified by gender and nutritional status. In this sense, health professionals will be able to use heart rate to estimate cardiovascular risk in children of different sexes and nutritional status.

Repeated-Dose Toxicity of Lauric Acid and Its Preventive Effect Against Tracheal Hyper-Responsiveness in Wistar Rats with Possible In Silico Molecular Targets

Indyra Alencar Duarte Figueiredo, Alissa Maria de Oliveira Martins, Alexya Mikelle Teixeira Cavalcanti, Jayne Muniz Fernandes, Ludmila Emilly da Silva Gomes, Mateus Mendes Vieira, Gabriel Nunes Machado de Oliveira, Isabela Motta Felício, Lucas Nóbrega de Oliveira, Igor Gabriel da Silva Ramalho, Natália Ferreira de Sousa, Luciana Scotti, Marcus Tullius Scotti, José Luiz de Brito Alves, Margareth de Fátima Formiga Melo Diniz, Daniele Idalino Janebro Ximenes, Luiz Henrique César Vasconcelos, Fabiana de Andrade Cavalcante 

DOI: 10.3390/ph18020221

Background/objectives: Lauric acid (LA), a medium-chain fatty acid, is a promising drug for asthma treatment. This study evaluated the toxicity of repeated doses and the effect of LA on pulmonary ventilation and tracheal reactivity in asthmatic Wistar rats and identified possible molecular targets of LA action in silico.

Methods: The rats were divided into control (CG) and LA-treated groups at 100 mg/kg (AL100G) for toxicity analysis. Pulmonary ventilation and tracheal reactivity were assessed in the control (CG), asthmatic (AG), asthmatic treated with LA at 25, 50, or 100 mg/kg (AAL25G, AAL50G, and AAL100G), and dexamethasone-treated groups (ADEXAG).

Results: The results showed that LA at a dose of 100 mg/kg did not cause death or toxicity. A pulmonary ventilation analysis indicated that AG had reduced minute volume, which was prevented in AAL25G. LA at all doses prevented carbachol-induced tracheal hyper-responsiveness and reduced the relaxing effect of aminophylline, as observed in AG. An in silico analysis revealed that LA had a good affinity for nine proteins (β2-adrenergic receptor, CaV, BKCa, KATP, adenylyl cyclase, PKG, eNOS, iNOS, and COX-2).

Conclusions: LA at 100 mg/kg has low toxicity, prevents hyper-responsiveness in an asthma model in rats, and acts as a multitarget compound with a good affinity for proteins related to airway hyper-responsiveness.

Repeated-Dose Toxicity of Lauric Acid and Its Preventive Effect Against Tracheal Hyper-Responsiveness in Wistar Rats with Possible In Silico Molecular Targets

Indyra Alencar Duarte Figueiredo, Alissa Maria de Oliveira Martins, Alexya Mikelle Teixeira Cavalcanti, Jayne Muniz Fernandes, Ludmila Emilly da Silva Gomes, Mateus Mendes Vieira, Gabriel Nunes Machado de Oliveira, Isabela Motta Felício, Lucas Nóbrega de Oliveira, Igor Gabriel da Silva Ramalho, Natália Ferreira de Sousa, Luciana Scotti, Marcus Tullius Scotti, José Luiz de Brito Alves, Margareth de Fátima Formiga Melo Diniz, Daniele Idalino Janebro Ximenes, Luiz Henrique César Vasconcelos, Fabiana de Andrade Cavalcante 

 DOI: 10.3390/ph18020221

Background/objectives: Lauric acid (LA), a medium-chain fatty acid, is a promising drug for asthma treatment. This study evaluated the toxicity of repeated doses and the effect of LA on pulmonary ventilation and tracheal reactivity in asthmatic Wistar rats and identified possible molecular targets of LA action in silico.

Methods: The rats were divided into control (CG) and LA-treated groups at 100 mg/kg (AL100G) for toxicity analysis. Pulmonary ventilation and tracheal reactivity were assessed in the control (CG), asthmatic (AG), asthmatic treated with LA at 25, 50, or 100 mg/kg (AAL25G, AAL50G, and AAL100G), and dexamethasone-treated groups (ADEXAG).

Results: The results showed that LA at a dose of 100 mg/kg did not cause death or toxicity. A pulmonary ventilation analysis indicated that AG had reduced minute volume, which was prevented in AAL25G. LA at all doses prevented carbachol-induced tracheal hyper-responsiveness and reduced the relaxing effect of aminophylline, as observed in AG. An in silico analysis revealed that LA had a good affinity for nine proteins (β2-adrenergic receptor, CaV, BKCa, KATP, adenylyl cyclase, PKG, eNOS, iNOS, and COX-2).

Conclusions: LA at 100 mg/kg has low toxicity, prevents hyper-responsiveness in an asthma model in rats, and acts as a multitarget compound with a good affinity for proteins related to airway hyper-responsiveness.

Impact of a Limosilactobacillus fermentum, Quercetin, and Resveratrol Nutraceutical on Fecal Microbiota Composition and Metabolic Activity in Healthy and Hypertensive Subjects

Jéssica Maria Alves Brasil, Nathalia Caroline de Oliveira Melo, Karoliny Brito Sampaio, Paulo César Trindade da Costa, Hatice Duman, Sercan Karav, Marcos Dos Santos Lima, Evandro Leite de Souza, José Luiz de Brito Alves

DOI: 10.3390/foods14060986

A promising strategy to improve the gut microbiome in hypertension is to target the gut microbiota. This study evaluated the effects of a potential nutraceutical product composed of three strains of Limosilactobacillus (L.) fermentum, quercetin, and resveratrol on the intestinal microbiome of healthy and hypertensive subjects. The nutraceutical product consisting of strains of L. fermentum 139, 263 and 296, fructooligosaccharides (200 mg), quercetin (160 mg), and resveratrol (150 mg) (LfQR) was added to the in vitro fecal fermentation process occurring for 48 h. Fecal samples of healthy and hypertensive subjects were allocated into four groups: (i) healthy controls (CTL); (ii) healthy controls with the addition of LfQR (CTL + LfQR); (iii) hypertensive (HTN) subjects; and (iv) hypertensive subjects with the addition of LfQR (HTN + LfQR). The diversity and composition of the fecal microbiota and the production of microbial metabolites were evaluated. CTL and HTN groups exhibited a distinct gut microbiota composition, as shown by the β-diversity assessment. The addition of the potentially nutraceutical-modulated β-diversity was similar between CTL and HTN groups, suggesting a similar gut microbiome composition after nutraceutical addition. The addition of the nutraceutical product increased the relative abundance of Enterobacteriaceae in the CTL group and that of Lachnospiraceae in the HTN group. The nutraceutical media showed higher levels of sugars (maltose, fructose, and glucose), lactic acid, ethanol, succinic acid, and acetic acid compared to the CTL and HTN media. Although the results are heterogeneous between healthy and hypertensive fermentation media, it was demonstrated that the nutraceutical formulation can modulate the intestinal microbiota and its metabolic activity.

Beneficial Effects of Omega-3 Fatty Acids on Obesity and Related Metabolic and Chronic Inflammatory Diseases

Donia Jerab, Ferdinand Blangero, Paulo César Trindade da Costa, José Luiz de Brito Alves, Rym Kefi, Henda Jamoussi, Beatrice Morio, Assia Eljaafari

DOI: 10.3390/nu17071253 

Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are known to help resolve inflammation through generation of anti-inflammatory eicosanoids and specialized pro-resolving mediators, including resolvins, protectins, and maresins. Through binding to the GPR120/FFAR4 receptor, their beneficial effects result from phospholipid membrane remodeling, impairment of inflammatory signaling molecules clustering, subsequent inhibition of NF-κB and inflammasome activation, and a reduction in oxidative stress. Obesity, a chronic inflammatory disease that contributes to metabolic disorders, is alleviated by n-3 PUFAs. In the adipose tissue (AT) of individuals with obesity, n-3 PUFAs counteract hypoxia, inhibit immune cell infiltration and AT inflammation, improve insulin sensitivity, and reduce fat mass. Beyond AT, n-3 PUFAs also alleviate other metabolic disorders such as metabolic-associated steatotic liver disease (MASLD), gut dysbiosis, and/or renal dysfunction. In cardiovascular disease (CVD), they are mainly recommended as a secondary prevention for patients with coronary heart disease risks. This review provides an in-depth analysis of the benefits of n-3 PUFAs in obesity and related metabolic diseases, examining both the mechanistic and clinical aspects. Additionally, it also explores the effects of n-3 PUFAs in obesity-related chronic inflammatory conditions, including inflammatory bowel disease, psoriasis, rheumatoid arthritis, osteoarthritis, and multiple sclerosis, by targeting specific pathophysiological mechanisms. Clinical applications and limitations of n-3 PUFAs are discussed based on findings from human clinical trials.

Protective effects of Hibiscus sabdariffa L. on body composition and airway reactivity in obese asthmatic rats

Sarah Rebeca Dantas Ferreira, Rayane Fernandes Pessoa, Alissa Maria de Oliveira Martins, Indyra Alencar Duarte Figueiredo, Bárbara Cavalcanti Barros, José Luiz de Brito Alves, Adriano Francisco Alves, Yuri Mangueira do Nascimento, Luiz Henrique César Vasconcelos, Fabiana de Andrade Cavalcante 

DOI: https://doi.org/10.1016/j.jff.2025.106757

Obesity worsens asthma symptoms and reduces treatment response, highlighting the potential of Hibiscus sabdariffa (HS), traditionally used for obesity management, as a treatment for obesity-exacerbated asthma. This study evaluated whether HS could prevent body composition, airway function, and reactivity changes in Wistar rats with obesity-exacerbated asthma. Male rats were divided into control (Ctrl), obese asthmatic (Ob + Asth), and HS-treated groups at 250, 500, and 1000 mg/kg. Rats received a high-glycemic diet for 16 weeks and were sensitized to ovalbumin in the last 22 days. HS was administered orally in the last 30 days. In vivo assessments included weight, fasting glucose, body mass index, and pulmonary ventilation, while in vitro tests measured tracheal contraction and lung and adipose histomorphometry. HS prevented weight gain, reduced glucose and adiposity, decreased adipocyte size, and limited tracheal hyperreactivity and peribronchovascular inflammation. These findings support HS as a promising option for treating obesity-exacerbated asthma.

Is low heart rate variability associated with cardiometabolic dysfunction in children with obesity? A cross-sectional study

Thallyta Alanna Ferreira Viana das Neves, Rúbia Cartaxo Squizato de Moraes, Davyson Barbosa Duarte, Joicy Karla Grangeiro Pereira, Paulo César Trindade da Costa, Cristiane Cosmo Silva-Luis, Francisco Antônio de Oliveira Júnior, Joelma Rodrigues de Souza, José Luiz de Brito Alves, Vinícius José Baccin Martins

DOI: 10.1016/j.numecd.2025.104116

Abstract

Background and aim: Children with obesity have impaired cardiac function and increased risk of cardiometabolic dysfunction. We investigated children with obesity and low heart rate variability (HRV) in terms of anthropometric parameters, insulin resistance, food addiction (FA), and blood pressure, comparing them with those who have normal heart rate variability.

Methods and results: Children aged 7-11 years, of both sexes, were divided into groups: the control group (CG, n = 34) comprised children with normal weight and normal heart rate variability; obesity plus normal heart rate variability (ONHRV, n = 51); and obesity plus low heart rate variability (OLHRV, n = 39). Anthropometric, biochemical parameters, HRV, blood pressure, and FA were measured. The OLHRV group showed higher weight (p < 0.001), waist circumference (p < 0.001), insulin resistance (p < 0.001), and lower RMSSD (p < 0.001) compared to ONHRV and CG. The prevalence of FA did not differ among the groups. Multiple regression analysis showed that the OLHRV group had a greater impact on insulin resistance (Beta 0.662, p < 0.001) than ONHRV (Beta 0.435, p < 0.001). Both groups explained systolic blood pressure (OLHRV, Beta 0.440, p < 0.001; ONHRV, Beta 0.434, p < 0.001).

Conclusion: Children with obesity plus low heart rate variability showed poor anthropometric indicators, increased insulin resistance, and an imbalance in the autonomic nervous system with low parasympathetic modulation. These findings suggest that the severity of obesity in children is associated with lower HRV and lower HRV plus obesity is an important predictor of insulin resistance and increased blood pressure.

Association of Depression with Cardiac Autonomic Function and Food Addiction in Individuals in Psychopharmacological Treatment 

Tainá Patrícia Teixeira Bezerra, Paulo César Trindade da Costa, Vinicius José Baccin Martins, Alfredo José Minervino, José Luiz de Brito Alves

DOI: 10.1007/s11469-025-01535-w

Abstract Major depressive disorder is a multifactorial condition and may be associated with food addiction and changes in cardiac autonomic dysfunction. This study investigated the occurrence of food addiction and cardiac autonomic function among individuals with depression and undergoing psychopharmacological treatment. The group depression (D) exhibited a higher body mass index (BMI) [mean 29.4 (5.7) vs. 26.4 (3.3); p=0.022] and greater number of food addiction symptoms [3 (0–9) vs. 1.5 (0–9); p=0.005] compared to the group control (C). Group D had lower LF (Low-Frequency band) (46.8±21.0 vs. 61.1±21.9), LF/HF ratio [0.78 (0.17–6.89) vs. 1.77 (0.15–6.67)], SD2/SD1 ratio (1.66±0.67 vs. 2.05±0.70), and DFA1 (0.90±0.35 vs. 1.15±0.25), but higher HF (High-Frequency band) (52.9±20.9 vs. 34.9±19.4). Furthermore, LF/HF ratio was positively correlated with depression severity (p=0.002; Spearman’s rho=0.558; effect size Fisher’s z=0.629). Regression models adjusted for potential confounders showed that depressive symptoms were positively associated with low-frequency (LF) components and food addiction symptoms and negatively associated with high-frequency (HF) components (p<0.05). Patients with depression exhibited changes in BMI, addictive eating symptoms, and cardiometabolic parameters, with a greater prevalence of parasympathetic tone.

Pleurotus Mushrooms in Nutrition and Health: Clinical and Preclinical Insights for Nutraceutical Development

Patrícia Lima Araújo, Ediana da Silva Araújo, Erika Mayra de Almeida Barreto, José Luiz de Brito Alves, Kamylla Mylena Souza, Micaelle Oliveira de Luna Freire, Rayane Maria Pessoa de Souza, Fillipe de Oliveira Pereira

 DOI: 10.1111/1541-4337.70279

Abstract

Pleurotus mushrooms are fungi widely consumed due to their high nutritional value and potential applications as nutraceuticals. Their sustainable cultivation and rich composition of bioactive compounds provide significant health benefits. This review examines the scientific evidence regarding the safety, efficacy, and nutraceutical potential of Pleurotus species, focusing on their effects on various human diseases. The review incorporates findings from preclinical, clinical studies, and nutraceutical formulations related to innovative Pleurotus-based products. Preclinical studies have shown that Pleurotus species can reduce inflammatory markers, modulate gut microbiota, and improve lipid and glucose metabolism. As a result, these mushrooms exhibit potential hypoglycemic, anti-obesity, hepatoprotective, neuroprotective, anti-atherogenic, and anticancer properties, along with possible benefits for preventing Alzheimer’s disease. Clinical trials suggest that consuming Pleurotus has a positive effect on metabolic parameters in healthy individuals and patients with chronic conditions. However, the variability among studies and the absence of standardized nutraceutical formulations hinder definitive conclusions about their therapeutic efficacy. Despite the promising potential of Pleurotus mushrooms in the nutraceutical sector, future research should focus on developing standardized formulations, optimizing bioavailability, expanding clinical trials, exploring the diversity of native species, and uncovering the underlying mechanisms of action to establish their practical application as nutraceuticals.

Effects of dietary energy restriction of ultra-processed foods compared to a generic energy restriction on the intestinal microbiota of individuals with obesity: a secondary analysis of a randomized clinical trial

Ana Debora Santos de Oliveira, Nayara Gomes Graciliano, Dafiny Rodrigues Silva, Mateus de Lima Macena, André Eduardo da Silva-Júnior, Micnéias Roberth Pereira, João Victor Laurindo Santos, Maria Bárbara Galdino Silva, Karine Maria Moreira Almeida, Déborah Tenório da Costa Paula, Débora Cavalcante Ferro, Guilherme César Oliveira de Carvalho, Marianna Victória Cerqueira Rocha, Natália Gomes da Silva Lopes, Samyra Araujo Monteiro de Carvalho, Fabiana Andréa Moura, José Luiz de Brito Alves, Bianca Luise Teixeira, Nassib Bezerra Bueno 

 DOI: 10.1039/d5fo02449h

Abstract

Evidence suggests that the intake of ultra-processed foods (UPF) influences gut microbiota. The present study evaluated the impact of energy restriction of dietary UPF compared to a general energy restriction on the gut microbiota of individuals with obesity. This was a parallel, randomized clinical trial. Individuals with obesity were randomly allocated into two groups: control, with general energy restriction (ER-G), and intervention, with energy restriction associated with UPF restriction (ER-UPF), limited to 5% of total intake. Individuals were followed-up monthly for 6 months. Stool samples were collected at baseline and after 6 months for DNA extraction and sequencing of the 16S rRNA gene (V3-V4 region). Socioeconomic, clinical, anthropometric, and dietary intake data were also collected. Abundance of microbiota data was analyzed using the Linear Models for Microarray Data (limma-voom) and the Microbiome Multivariable Associations with Linear Models (MaAsLin2) packages, with the Benjamini-Hochberg correction method. Alpha-diversity was analyzed using the Shannon, Simpson and Simpson-inverse index, whereas beta-diversity was analyzed using the Bray-Curtis index with the PERMANOVA test. Other variables were analyzed using mixed ANOVA with an alpha level of 5%. A total of 43 individuals were included, and at the end of the study, 34 individuals (18 intervention and 16 control) had complete data and were analyzed. %UPF consumption significantly decreased in the group that restricted UPF (p-interaction = 0.01), whereas waist circumference and body fat decreased in both groups (p-moment < 0.01). It was observed an increase in phylum and family microbial alpha-diversity in both groups with a slight decrease at the genus level. The Ruminococcaceae family showed a significantly greater increase in the ER-UPF group compared to the control group (p-interaction = 0.03), as did the Faecalibacterium genus (p = 0.02). These findings highlight the complexity of interactions between diet, body composition, and gut microbiota, suggesting that UPF restriction may have positive but limited effects on microbiota modulation in the short to medium term. Registration number of Clinical Trial: RBR – 3q9vgk9.

Early introduction of ultra-processed foods and their repercussions on inflammatory biomarkers in children

Cecília Furtado Craveiro, Mariana Souza Lopes, Bruna Farias da Silva, Fernando Paiva Brandini, Erika Mayra de Almeida Barreto, Pietra Maria Pereira de Lima, Ricácia de Sousa Silva, Sávio Marcelino Gomes, Vinicius José Baccin Martins, José Luiz de Brito Alves 

DOI: 10.1177/02601060251397284

Abstract

Background: Excessive consumption of ultra-processed foods (UPFs) has been associated with adverse health outcomes, including increased levels of inflammatory biomarkers such as C-reactive protein (C-RP), IL-6, and TNF-α. Aims: To investigate whether the introduction of UPFs before the age of 2 years is associated with inflammatory markers in school-aged children (7-10 years). Methods: This cross-sectional study included interviews with children and their mothers conducted by a trained team. Anthropometric and biochemical data (C-RP), IL-2, IL-4, IL-6, IL-10, IL-17a, IFN-γ, and TNF-α) were collected from children. Maternal reports provided information on family income, schooling, breastfeeding (exclusive up to 6 months), timing of food introduction, early exposure to UPFs (before 2 years), and birth weight. Statistical analyses included PERMANOVA and generalized estimating equations, adjusted for family income, BMI-for-age, and exclusive breastfeeding. Summary: The prevalence of early UPF introduction was higher among girls compared to boys (54.7% vs. 45.3%, P < 0.001). Children exposed to UPFs before 2 years of age had higher IL-10 levels (P = 0.002), but this association was not sustained after adjustments. Early UPF consumption (before 2 years of age) was not associated with alterations in inflammatory cytokines in children aged 7-10 years. Nevertheless, considering the established links between UPF intake, metabolic disorders, and neurodevelopmental outcomes, continuous monitoring and dietary guidance during childhood remain essential to promote lifelong health.

Synbiotic Nutraceutical Mitigates Gestational Diabetes Effects and Cardiovascular Dysfunction in Rat Offspring

Deborah Emanuelle de Albuquerque Lemos, Evandro Leite de Souza, Micaelle Oliveira de Luna Freire, Jaielison Yandro Pereira da Silva, Paulo César Trindade da Costa, Josiane de Campos Cruz, Alisson Macário de Oliveira, João Felipe Mota, José Luiz de Brito Alves

 DOI: 10.1002/mnfr.70340

Abstract

This study investigated the effects of a nutraceutical combining jabuticaba peel (Myrciaria jaboticaba (Vell.) Berg) and Limosilactobacillus fermentum strains on gut microbiota, glycaemic and lipid profiles, and inflammatory markers in rats with gestational diabetes mellitus (GDM), as well as its effects on cardiac autonomic function in the offspring. Pregnant Wistar rats were assigned to control (CTL), GDM, and GDM receiving the nutraceutical (GDM+Nut). The nutraceutical (2 g/kg) or vehicle (PBS) was administered twice daily. After weaning, the offspring were fed laboratory chow until 80 days of age. Nutraceutical administration improved glucose tolerance and reduced serum inflammatory cytokines (TNF-α and IL-6). Beta diversity was increased without altering alpha diversity indices in the GDM+Nut. Nutraceuticals increased the abundance of short-chain fatty acid-producing bacteria. Maternal supplementation protected against autonomic dysfunction in the offspring. Nutraceutical supplementation shows potential as an alternative therapy for the treatment of GDM, while mitigating autonomic dysfunction in offspring.

Development and functional stability of a next-generation nutraceutical combining Pleurotus djamor mushroom and Limosilactobacillus fermentum strains 

Kamylla Mylena Souza Barbosa, Evandro Leite de Souza, Francinalva Dantas de Medeiros, Giuliane Moura Andrade, Jaielison Yandro Pereira da Silva, José Luiz de Brito Alves, Jhonatan Rafael Zarate-Salazar, Marcos dos Santos Lima, Maria Elieidy Gomes de Oliveira, Natalia Sufiatti Holanda Cavalcanti, Maiara da Costa Lima, Yasmim Fonseca Simplicio da Silva, Fillipe de Oliveira Pereira

DOI: 10.1016/j.fbp.2025.11.026 

This study reports the development and evaluation of the viability and functional stability of potentially symbiotic nutraceuticals formulated with the edible mushroom Pleurotus djamor and probiotic Limosilactobacillus fermentum (139, 263, and 296). The nutraceutical (NPD) was produced by combining P. djamor powder with L. fermentum strains and freeze-drying, and its physicochemical properties, probiotic viability, and stability were assessed under storage and simulated gastrointestinal digestion. NPD presented high protein and fiber content, low fat, and phenolic compounds (epigallocatechin gallate, catechin, and epicatechin) associated with antioxidant activity. After 90 days, the viable cell counts decreased significantly at both 4 ± 0.5 ◦C (NPD4) and 25 ± 0.5 

Impact of Maternal Nutritional Status and Mental Health on Children with Obesity: Relationship Between Anthropometric Parameters, Food Addiction, and Stress

Joicy Karla Grangeiro Pereira, Rúbia Cartaxo Squizato de Moraes, Thallyta Alanna Ferreira Viana das Neves, Davyson Barbosa Duarte, Cristiane Cosmo Silva-Luis, Paulo César Trindade da Costa, Carla Lúcio Alves, Melyssa Kellyane Cavalcanti Galdino, Carla Alexandra da Silva Moita Minervino, Nassib Bezerra Bueno, José Luiz de Brito Alves, Vinicius José Baccin Martins

DOI: 10.3390/ijerph22091312

Abstract

Childhood obesity is a complex and multifactorial disease influenced by various factors including behavioral, physical, and psychological conditions. This cross-sectional study aimed to evaluate the impact of maternal and child stress and food addiction on the nutritional status of children with obesity. Children aged 6 to 12 years were divided into Control Group (n = 42) and Obesity Group (n = 68) according to BMI-Z score. Mothers were allocated to their children’s respective groups. Anthropometric measures, body composition, stress levels, and food addiction were evaluated in both children and mothers. Children with obesity exhibited a significantly higher prevalence of food addiction compared to controls (22.1% vs. 2.6%, p = 0.006), while mothers in the control group showed higher stress levels (68.6% vs. 46.3%, p = 0.039). Positive correlations were observed between mother-child pairs for weight (r = 0.433, p < 0.01) and waist circumference (r = 0.461, p < 0.01). In children, food addiction was a significant predictor of BMI-Z scores (adjusted R square = 0.186); however, maternal BMI and stress were more important predictors (adjusted R square = 0.468). These findings highlight the influence of maternal physical and psychological health on childhood BMI-Z scores. Effective interventions should target both mother and child to improve overall health outcomes.

Supplementation with Lactiplantibacillus plantarum CNPC003 and Pilosocereus gounellei Flour Enhances the Properties of Goat Cream Cheese

Daniela Karla Medeiros Vasconcelos, Evandro Leite de Souza, Márcia Gabrielle Silva Viana, Maria Isabel Ferreira Campos, Lorena Lucena de Medeiros, Lary Souza Olegário, Mércia de Sousa Galvão, Karina Maria Olbrich dos Santos, Antônio Silvio do Egito, Marta Suely Madruga, Marcos dos Santos Lima, Tatiane Santi Gadelha, Maria Teresa Bertoldo Pacheco, Kataryne Árabe Rimá de Oliveira, Maria Elieidy Gomes de Oliveira

DOI: 10.3390/microorganisms13020254

Abstract

This study evaluated the impacts of Lactiplantibacillus plantarum CNPC003 and xique-xique flour supplementation on the technological, physicochemical, nutritional, and sensory properties of goat cream cheese over 21 days of refrigerated storage. Four cheese formulations were prepared: a control (CC), one with L. plantarum CNPC003 (PC), one with xique-xique flour (XC), and one with L. plantarum CNPC003 and xique-xique flour (PXC). XC and PXC had a yellowish-green hue with less brightness. PC and PXC were less firm and adhesive with greater elasticity, cohesiveness, and gumminess, and they had reduced total protein and increased total free amino acids (p < 0.05) during storage. The contents of specific volatile compounds increased in PXC during storage. PXC had higher L. plantarum counts than PC on day 21 of storage. PC and PXC had distinct colors and textures and were well accepted regarding sensory attributes. Xique-xique flour and L. plantarum CNPC003 supplementation positively impact the nutritional and functional characteristics of goat cream cheese without negatively affecting the technological and sensory attributes.

A novel coating formulated with sodium alginate loaded with a mixed lactic acid bacteria culture to control anthracnose in guava and mango

Karina Felix Dias Fernandes, Kataryne Árabe Rimá de Oliveira, Ítallo Fernandes Cirilo, Maiara da Costa Lima, Marcos Paz Saraiva Câmara, Evandro Leite de Souza 

DOI: https://doi.org/10.1016/j.foodcont.2025.111341

Guava and mango are tropical fruits widely consumed due to their pleasant flavor and nutritional composition. Anthracnose caused by Colletotrichum spp. Is considered a primary post-harvest disease affecting these fruits. Applying biocontrol agents, such as lactic acid bacteria (LAB), combined with coating materials is an innovative fruit biopreservation method. This study investigated the effects of a novel sodium alginate coating loaded with a mixed culture of LAB in controlling anthracnose caused by different pathogenic Colletotrichum isolates in guava (cv.) Paluma and mango (cv.) Palmer during 15 days of room temperature storage (25 ± 0.5 ◦C). A mixed culture formed by three different LAB species strains (Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263) was tested against ten target Colletotrichum isolates. The mixed culture inhibited the mycelial growth of all target Colletotrichum isolates in vitro, with up to 100 % inhibition. In the nutrient competition method, the mixed LAB culture survived and caused mycelial growth inhibition even at low nutrient concentrations. The coatings showed sizes of LAB cell subpopulations with non-permeabilized membrane (viable cells, PI-CFDA+) of >31 % at the end of refrigerated storage and viable cell counts of >7 log CFU/ g. Applying coatings with sodium alginate loaded with mixed LAB culture delayed the development and decreased the severity of anthracnose lesions in guava and mango artificially contaminated with either of the tested Colletotrichum isolates. The coating formulated with sodium alginate and mixed LAB culture can be effective for postharvest anthracnose biocontrol in guava and mango.

Preparation and Characterization of Cornstarch and Sodium Alginate Edible Films Incorporated with Pink Pepper Essential Oil (Schinus Terebinthifolius)

Larruama P. F. de V. Lino, José M. Pereira Filho, Abrahão A. de Oliveira Filho, Bruna S. Araujo Stella A. de Figueiredo Rosália S. de Medeiros Leilson R. Bezerra Juliana P. F. de Oliveira Kataryne Á. R. de Oliveira Ronaldo Lopes Oliveira 

DOI:  10.1007/s41783-025-00189-2

The food industry is responsible for approximately 40% of global plastic production. This is mainly due to the extensive use of plastic in packaging, which plays a significant role in environmental degradation. This study aimed to develop and characterize biopolymeric films based on a corn starch and sodium alginate filmogenic solution (FS) incorporated with pink pepper essential oil (PPO). Using the casting method, films were prepared based on a completely randomized experimental design, incorporating four formulations with PPO concentrations of 0% (control), 1.0%, 1.5%, and 2.0%. The films were evaluated for color, opacity, thickness, mechanical and barrier properties, morphology, thermogravimetry, toxicity, as well as antioxidant and antimicrobial activities. The results revealed that the films exhibited a uniform structure, with the addition of PPO leading to a more homogeneous microstructure (P < 0.05) at the 1% inclusion level and improved thermal stability up to the 1.5% PPO concentration, compared to the control films. Additionally, there was an increase (P < 0.05) in redness and yellowness, as well as water vapor permeability (WVP) in films containing 1.5% and 2% PPO. Conversely, PPO incorporation resulted in a decrease (P < 0.05) in opacity and tensile strength, while enhancing (P < 0.05) elasticity. No significant changes were observed in thickness or luminosity, and the films did not exhibit antimicrobial activity against S. aureus and E. coli strains (P < 0.05). Based on these findings, the inclusion of 1.0% PPO is recommended for optimal film performance, although further research is necessary to explore its full potential for application in the agri-food industry.

Controlling anthracnose in mango and guava with antifungal coatings of sodium alginate containing hydroalcoholic extracts of fruit by-products fermented by lactic acid bacteria

Talita Silveira Queiroga, Kataryne Árabe Rimá de Oliveira, Karina Felix Dias Fernandes, Jaielison Yandro Pereira da Silva, Jéssica Silva Freire, Jociano da Silva Lins, Francisco Humberto Xavier-Júnior, Josean Fechine Tavares, Evandro Leite de Souza

 DOI: 10.1093/jambio/lxaf219

Aim: This study evaluated the efficacy of hydroalcoholic extracts from fruit by-products fermented by lactic acid bacteria (LAB), i.e. a mix of Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263, as active antifungal agents in sodium alginate coatings for the control of anthracnose caused by Colletotrichum spp. in mango cv. Palmer and guava cv. Paluma.

Method and results: The hydroalcoholic extracts of fermented and unfermented acerola, cashew, mango, and grape by-products presented variations in acidity, pH, total soluble solids, ascorbic acid, total phenolic compounds, flavonoids, and antioxidant activity. Sixteen chemical constituents were identified, including sugars and organic molecules, in the hydroalcoholic extracts of fermented and unfermented acerola and mango by-products. Hydroalcoholic extracts of fermented acerola and mango by-products caused the highest mycelial growth inhibition % (%MGI: 19.5%-76.31%) against Colletotrichum spp. using the agar-well diffusion method. The fermented extracts (1 g.L-¹) caused the most promising inhibition (%MGI: 35.00%-82.75%) and were identified as the minimum inhibitory concentration. Coatings containing these extracts delayed the development and severity of anthracnose in mango and guava during 15 days of storage (25°C), with a % anthracnose lesion diameter reduction (%ALDR) between 3.93 and 87.01%.

Conclusion: Hydroalcoholic extracts from fermented acerola and mango by-products exhibited notable antioxidant and antifungal activity. Their incorporation into sodium alginate coatings represents an innovative strategy to control postharvest anthracnose in fruits.

Functional activities and applications of Pequi Oil: a systematic review and meta-analysis 

Stella de Alencar FIGUEIREDO, Leilson Rocha BEZERRA, Marthyna Pessoa de SOUZA, Edson Cavalcanti da SILVA FILHO, Kataryne OLIVEIRA, Abrahão Alves de OLIVEIRA FILHO, André SILVA, Rosália Severo de MEDEIROS, Juliana Paula Felipe de OLIVEIRA

DOI:10.5327/fst.485

Pequi oil is a promising raw material known for its high content of unsaturated fatty acids, polyphenols, and carotenoids. This systematic review focuses on its potential and industrial applications. A total of 27 studies were selected, and 14 were meta-analyzed. The results demonstrated the oil’s potential in the food industry, where natural antioxidants served as preservatives, stabilizers, and additives, thereby extending the shelf life of products like yogurt and cheese. Additionally, pequi oil has applications in pharmaceuticals, phyto-cosmetics, as well as in nano-emulsions and nanocomposites. The metaanalysis revealed a total effect size of 0.73 with a 95% confidence interval of 0.67–0.79 and a significant p-value (p < .01). Heterogeneity was observed (I2 = 95%; p < .0011), supporting the practical application of pequi oil in various therapeutic interventions. These include antioxidant, anti-inflammatory, antigenotoxic, and anticarcinogenic effects, as well as potential treatments for diseases related to oxidative stress, glycemic control, anemia, reduction of low-density lipoproteins cholesterol, osteoarthritis symptoms, and dermal wound healing. Pequi oil shows considerable potential for product development across various industrial sectors. However, it is essential to explore alternative methods of its exploitation to expand its practical applications and foster new product development.

Exploring the Potential of Lactic Acid Bacteria as Biotechnological Tools for the Control of Phytopathogenic Fungi in Fruits and Vegetables: A Review of Preliminary Evidence

Kataryne Árabe Rimá de Oliveira, Karina Felix Dias Fernandes, Maiara da Costa Lima, Natália de Souza Bento, Viviane Priscila Barros de Medeiros, Evandro Leite de Souza

DOI: 10.1111/1541-4337.70323

Fruits and vegetables exist in a dynamic environment and are constantly exposed to biotic threats. Fungi are important phytopathogens that can infect different tissues at any stage of plant development. The development of fungal infections results in decreased productivity, changes in appearance, and reduced overall consumer acceptance. Additionally, some phytopathogenic fungi produce mycotoxins that can harm humans and animals. Chemical methods, such as fungicides and chemical preservatives, have been traditionally used to control fungi in fruits and vegetables with varying degrees of success. However, these methods can pose health, safety, and environmental concerns, resulting in the search for a safer alternative strategy. Lactic acid bacteria (LAB) are promising and effective candidates for post-harvest disease management, showing inhibitory effects against several phytopathogens. The antagonistic effects of LAB against phytopathogens are diverse and multifaceted, including competition for space and nutrients, parasitism, and the production of inhibitory metabolites such as acids and volatile organic compounds. Parameters indicating the post-harvest quality of fruits and vegetables can also be preserved by applying LAB. However, some practical challenges may limit the broad application of LAB in fruits and vegetables, including host specificity, sensory changes, environmental cultivation conditions, production costs, complex regulatory approval, and the need for adequate characterization of antifungal compounds. Additionally, limited field trials and a lack of standardized protocols hinder commercial application, highlighting the need for further research to optimize LAB use in sustainable crop protection. The information presented in this review highlights new perspectives on exploring LAB and their metabolites for controlling phytopathogenic agents in fruits and vegetables.

Lactiplantibacillus plantarum as a promising adjuvant for neurological disorders therapy through the brain-gut axis and related action pathways.

Josilaene Duarte Luiz, Cynthia Manassi, Marciane Magnani, Adriano Gomes da Cruz, Tatiana Colombo Pimentel, Silvani Verruck

DOI: 10.1080/10408398.2023.2280247 

Dysbiosis in neurological disorders has highlighted the gut-microbiota-brain axis and psychobiotics and their ability to act on the brain-gut axis. Studying and discovering new approaches in therapies for neuropsychiatric disorders are strategies that have been discussed and put into practice. Lactiplantibacillus plantarum is a lactic acid bacteria species with an extensive history of safe use whose action as a psychobiotic has been successfully explored. This review describes and discusses the mechanisms of action of L. plantarum and its potential for the prevention and treatment of neurological disorders. Randomized and controlled trials in humans or animals and using supplements based on different strains of L. plantarum were selected. The psychobiotic effect of L. plantarum has been shown, mainly through its action on the Hypothalamic-Pituitary-Adrenal (HPA) axis and regulation of levels of pro-inflammatory cytokines. Furthermore, it could protect the integrity of the intestinal barrier and decrease inflammation, alleviating a series of symptoms of neurological diseases. The results showed improvements in cognitive function, memory, anxiety, hyperactivity, Attention Deficit Hyperactivity Disorder (ADHD), sleep quality, and growth stimulation of beneficial species of bacteria in the gut. Larger and deeper studies are needed to use psychobiotics to prevent and treat neurological disorders.

SARS-CoV-2 surrogate bacteriophage φ6 cross-contamination between fruits and gloves, survival on discarded gloves and inactivation by photodynamic treatment.

Ruthchelly Tavares da Silva, Alyson José dos Santos Franco, Maria Mayara de Souza Grilo, Atila Lima, Karina Lidiane Alcântara Saraiva, Rafaela de Siqueira Ferraz Carvalho, Geany Targino de Souza Pedrosa, Donald W. Schaffner, Marciane Magnani 

DOI:10.1016/j.fm.2024.104645

This study assessed the SARS-CoV-2 surrogate bacteriophage φ6 cross-contamination between high-density polyethylene or polyvinyl chloride gloves and fruits (tomato and cucumber) using different inoculum levels (6.0 and 4.0 log PFU/sample). Bacteriophage φ6 survival on contaminated gloves was assessed over 9 days at 25 °C. The effectiveness of photodynamic treatment using curcumin as a photosensitizer to inactivate φ6 on fruits was determined. The fruit type and the glove material influenced the φ6 transfer. Longer contact times resulted in greater φ6 transfer. The highest φ6 transfer occurred from tomato to HDPE glove (0.8% or −1.1 log % transfer) after 30 s of contact at the higher inoculum level. Bacteriophage φ6 was detected on cross-contaminated HDPE gloves for up to 6 days. Bacteriophage φ6 survived better on vinyl gloves cross-contaminated by cucumber vs. tomato (detected up to 6 vs 3 days). Photodynamic inactivation of φ6 was time-dependent and varied with the tested fruit but was not influenced by viral starting concentration. Photodynamic treatment decreased the φ6 titer by 3.0 and 2.2 log PFU/sample in tomato and cucumber, respectively. Transmission electronic microscopy showed that photodynamic treatment changed the structure of the φ6 capsid. These findings may help in the management of SARS-CoV-2 contamination risks in fruit handling. They 

may also help in the establishment of effective measures to manage cross-contamination risk.

Development and characterization of microencapsulated Pichia kluyveri CCMA 0615 with probiotic properties and its application in fermented beverages.

Iara Ferreira Resende, Pâmela Mynsen Machado Martins, Dirceu de Souza Melo, Marciane Magnani, Disney Ribeiro Dias, Rosane Freitas Schwan

DOI: 10.1016/j.ijfoodmicro.2024.110967

The study aimed to develop innovative microencapsulated formulations of strains with probiotic attributes, Pichia kluyveri CCMA 0615 and Saccharomyces cerevisiae CCMA 0732. The yeasts (8 log CFU/mL) were microencapsulated by spray drying technique using whey powder (WP – 15 %, 20 %, and 30 %) and sodium alginate (ALG – 1 %). The microcapsules and cell viability were characterized during two months of storage (4 °C and 25 °C). The selected formulations were applied to functional beverage fermentation, and viability and survival in the simulated gastrointestinal tract (GIT) were performed. The viability of yeasts microencapsulated by the spray drying method was shown to be dependent on the strain and encapsulating matrix used, ranging from 84 to 99 %. P. kluyveri required refrigeration when storing microcapsules. In functional beverage fermentation, microencapsulated yeast maintained the same fermentative profile with carbohydrate consumption, production of lactic acid (0.30 to 1.10 g/L) and alcohol (0.2 to 1.61 g/L), and greater viability during storage. Finally, the microencapsulation of P. kluyveri with 15 % WP + 1 % ALG maintained high viability under GIT conditions, whether exposed independently (>84 %) or incorporated into a food matrix (>94 %). The study demonstrated that this innovative microencapsulation of probiotic yeasts increases their viability, improves biotechnological application, and facilitates efficient delivery of probiotics to the host.

Malícia honey (Mimosa quadrivalvis L.) produced by the jandaíra bee (Melipona subnitida D.) shows antioxidant activity via phenolic compound action in obese rats.

Maria Luiza Rolim Bezerra, Mirela Gouveia-Nhanca, Maria Letícia da Veiga Dutra, Kamila Sabino Batista, Alana Natalícia Vasconcelos de Araújo, Marcos Dos Santos Lima, Mateus Duarte Ribeiro, Alexandre Sergio Silva, Adriano Francisco Alves, Tatiana Colombo Pimentel, Marciane Magnani, Jailane de Souza Aquino

DOI:  10.3389/fnut.2025.1524642

Background and aims: Obesity is a disease associated with increased oxidative stress in humans and animals, and consumption of antioxidant compounds such as polyphenols can minimise it. These compounds are abundant in malícia (Mimosa quadrivalvis L.) honey produced by stingless bees. This study aimed to evaluate whether administration of Mimosa quadrivalvis L. honey to obese rats could reduce oxidative stress in vital organs through phenolic compound action. Methods: Wistar rats (228 ± 14.69 g) were randomly divided into two groups: a healthy group (HG, n = 20) fed a control diet and an obese group (OG, n = 20) fed a cafeteria diet for the initial 8 weeks. After this period, these groups were again randomised into four subgroups: healthy (HG, n = 10), obese (OG, n = 10), healthy with malícia honey administration (1,000 mg/kg; HGH, n = 10), and obese with malícia honey administration (1,000 mg/kg; OGH, n = 10) for the final 8 weeks fed the previously mentioned diets. The rats were euthanised at the end of the experiment to collect brain, gut, kidney, and liver tissues to evaluate parameters related to oxidative stress and phenolic profile. Results: The administration of malícia honey reduced energy intake and weight gain in the OGH in comparison to the OG. Total antioxidant capacity increased in the brain, liver, and gut in both groups treated with honey compared to respective controls. Lipid peroxidation decreased in the brain, gut, and kidney of the OGH. Both treated groups showed elevated phenolic compound deposition, including catechin, procyanidins, and flavonoids, across all organs. Specifically, the brain in the OGH showed greater procyanidin B2 and gallic acid deposition; the liver showed increased procyanidin B1 and B2, epicatechin, and myricetin concentrations; the gut showed higher procyanidin B2 and kaempferol 3-glucoside concentrations; and the kidneys had increased catechin, procyanidin B1 and B2, and gallic acid deposition compared to the OG. Conclusion: Histologically, the OGH displayed reduced neuronal damage and prevention of hepatic steatosis induced by the cafeteria diet. Malícia honey effectively reduced oxidative stress via modulation of phenolic compounds in the brain, gut, kidney, and liver of cafeteria diet-induced obese rats.

Transfer, survival and photoinactivation of Salmonella enterica on fresh produce and gloves.

Ruthchelly Tavares da Silva, Geany Targino de Souza Pedrosa, Alyson José Dos Santos Franco, Maria Mayara de Souza Grilo, Fernando Azevedo de Lucena, Carlos Eduardo Barão, Jiin Jung, Donald W Schaffner, Marciane Magnani 

DOI: 10.1016/j.ijfoodmicro.2025.111089

This study quantified the transfer of Salmonella between fruit (tomato and cucumber) and gloves (High-density polyethylene-HDPE, Vinyl-VY) after different contact time (2, 5, 10 and 30 s). The survival of Salmonella in discarded gloves and the photodynamic inactivation (PDI; curcumin 7.5 μM; 16.1, 20.2 and 24.2 J/cm2) of the pathogen were also evaluated. Scanning electronic micrographs showed the cell morphology of Salmonella on discarded HDPE and VY gloves. The highest transfer (p > 0.05) of Salmonella occurred after 30 s (4.0 %; -1.4 log %) from tomato to HDPE. Salmonella on contaminated gloves survived for up to 9 days (2.1-1.5 log CFU/sample). PDI reduced (p > 0.05) Salmonella counts ∼3.0 and 2.0 log CFU/sample after 12 min on tomato and cucumber, respectively. These results contribute important information about transfer and survival of Salmonella on gloves and fruit. The obtained data can be used to support the development of risk assessment models and measures to mitigate the risks of Salmonella contamination in fresh- produce.

Characterization and biological activity of ultrafiltrate goat whey protein concentrate over the in vitro digestion.

Karla Kaligia Silva Borba, Carlos Gadelha, Katherine Gutiérrez-Álzate, Luciano da Silva Pinto, Marta Sueli Madruga, Maria Teresa Bertoldo Pacheco, Renata Maria Teixeira Duarte, Rita de Cássia Ramos do Egypto Queiroga, Marion Pereira da Costa, Marciane Magnani, Tatiane Santi Gadelha

DOI:10.1016/j.fbio.2025.106087

This study aimed to characterize the peptide profile of a goat whey protein concentrate subjected to in vitro gastrointestinal digestion using pepsin and pancreatin enzymes and evaluate the resulting hydrolysates’ antioxidant and antimicrobial properties. The whey protein concentrate was obtained through ultrafiltration and analyzed across three stages: undigested (ND), gastric digested (GD), and gastrointestinal digested (GID). Molecular size and protein composition were assessed using SDS-PAGE/Tricine electrophoresis. Hydrophobicity was evaluated via column chromatography, and free amino acid profiles were determined. Antioxidant activity was measured using DPPH−+ and ABTS−+ assays. In contrast, antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration against foodborne pathogens, spoilage bacteria and yeast. The results revealed that α-lactoalbumin was degraded in the GD phase, whereas β-lactoglobulin showed significant degradation after pancreatin action in the GID phase. High molecular weight (>52 kDa) whey proteins formed diffuse bands after enzymatic action. Chromatographic analysis showed peptide release in the GID phase and a reduction of peaks in goat whey (3.42 ND to 0.34 GID). The GID phase exhibited a significant increase (p < 0.05) in the release of essential (56%–80.9%) and branched chain (6%–15%) amino acids. GID peptides showed the highest antioxidant activities with 64.68% (ABTS−+) and 47.95% (DPPH−+) inhibition and the strongest activity against Escherichia coli and Listeria innocua. These findings underscore the bioactive potential of goat whey protein hydrolysates following simulated gastrointestinal digestion, highlighting their promise as functional ingredients with antioxidant and antimicrobial properties.

Freshwater microalgae biomasses are a source of bioaccessible bioactive compounds and have antioxidant, antihypertensive, and antidiabetic activity.

Francyeli Araújo Silva, Renally Lima dos Santos, Carlos Eduardo Barão, Viviane Priscila Barros de Medeiros, Thiago Okagawa Silva, Cristiane Francisca da Costa Sassi, Andresa Carla Feihrmann, Marcos dos Santos Lima, Tatiana Colombo Pimentel, Marciane Magnani 

DOI: 10.1016/j.foodres.2025.116259

Due to their sustainable production, freshwater microalgae have received attention as an alternative food rich in nutrients and bioactive compounds. This work assessed the composition and bioaccessibility of amino acids (AA), fatty acids (FA), and phenolic compounds (PC) present in the Chlorella vulgaris, Spirulina platensis, Scenedesmus acuminatus, Desmodesmus maximus, and Tetraselmis sp. biomass, and their antioxidant, antihypertensive and antidiabetic in vitro activities. The microalgae had a similar profile of compounds, but the concentration varied significantly between species. Lysine was the most abundant essential AA and palmitic and oleic (ω9) acids were the main FA detected in all microalgae. Moreover, the bioaccessibility was higher than 70 % for most FA, while the bioaccessibility of PC was generally low (<20 %). All microalgae showed significant antidiabetic (∼20–54 % ⍺-amylase inhibition and ∼ 91 % to ∼96 % ⍺-glucosidase inhibition), antihypertensive (∼13–53 % ACE inhibition) and antioxidant (1313.10–1821.60 μM eq Trolox/g) potential. C. vulgaris showed a higher concentration and bioaccessibility of phenolic compounds, resulting in an increased antidiabetic activity (higher α-glucosidase inhibition). S. platensis showed higher concentrations of fatty acids (palmitic, palmitoleic, and linoleic). D. maximus was characterized by higher concentrations and bioaccessibility of amino acids and an increased antioxidant and antidiabetic activities. Finally, Tetraselmis sp. presented improved concentration of tyrosine and increased antihypertensive activity. Therefore, the results can support the application of microalgae biomass in new functional foods for delivering bioactive compounds, primarily essential AA and FA, targeting specific niches that benefit from their potential health advantages. Findings underscore the commercial applicability of microalgae-based products.

Fermentation of Amazonian fruit pulp (bacaba) with distinct probiotics: impacts.

Bianca Beatriz Torres de Assis, Lucélia Cabral, Francyeli Araújo Silva, Jaqueline de Araújo Bezerra, Melline Fontes Noronha, Hubert Vidal, Marcos Dos Santos Lima, Tatiana Colombo Pimentel, Marciane Magnani

DOI:  10.1016/j.foodres.2025.116326

Bacaba (Oenocarpus bacaba Mart.) is an underexplored Amazonian fruit rich in polyphenols that can serve as a substrate for probiotic survival and may positively impact on the composition and metabolism of the intestinal microbiota. This study aimed to evaluate the bacaba pulp fermented with probiotics Lactobacillus acidophilus 05 (LA-05) and Lacticaseibacillus casei 01 (LC1) regarding the chemical composition and probiotics survivability during fermentation (48 h), and the effect on the modulation of the intestinal microbiota of healthy adults through 16S rRNA sequencing. The probiotic-fermented bacaba pulps showed decreased pH and total soluble solids values and sugar content (maltose, glucose, fructose, and rhamnose), and increased titratable acidity values, organic acid content (lactic and tartaric acids), and phenolic compounds concentration compared to the control pulp. Furthermore, it presented adequate probiotic viability after fermentation and simulated gastrointestinal conditions. The bacaba pulp fermented with LC1 showed a higher concentration of butyric acid and phenolic compounds concentration (trans-resveratrol, cis resveratrol, catechin, procyanidin B2, and pelargonidin 3-glucoside) and bioaccessibility compared to the control pulp. The bacaba pulp fermented with LA-5 showed a higher concentration of pelargonidin 3-glucoside and procyanidin B2 compared to the control pulp and the highest bioaccessibility of some phenolic compounds (trans-resveratrol, cis-resveratrol, catechin, epicatechin, procyanidin B1, procyanidin B2, myricetin, and isorhamnetin). In vitro fecal fermentation reduced the pH and increased the abundance of Desulfovibrionales, Lactobacillales, and Peptostreptococcales-Tissierellales for all treatments. Bacaba pulp with LC1 resulted in the lowest pH values, and increased production of organic acids and concentration of phenolic compounds. Furthermore, both probiotic pulps increased the abundance of Lactobacillales and Acidaminococcales and decreased the abundance of Clostridiales. These findings provide new information about the potential of using bacaba in a functional pulp that may benefit human health through colonic microbiota changes.

Understanding food safety on sprouts and microgreens: contamination routes, outbreaks and challenges.

Isabella Bassoto Xavier, Jerfferson de L. Tavares, Edson Douglas Silva Pontes, Marciane Magnani, Verônica Ortiz Alvarenga 

DOI: 10.1016/j.foodres.2025.116589

The consumption of micro-scale vegetables, such as sprouts and microgreens, has become popular in recent years. Due to their high nutritional content and rapid production, they are becoming increasingly present in the diet. However, cultivation conditions, such as high humidity and high nutritional content, favor the development of pathogens, such as Salmonella enterica, Escherichia coli and Listeria monocytogenes. Thus, the factors that contribute to microbial contamination, and decontamination strategies were investigated in this review. Although many of these treatments have demonstrated potential in reducing microbial contamination, challenges, particularly in managing viral contamination and determining the long-term effectiveness of current approaches, remain. A critical approach is to develop more effective safety protocols and support the role of sprouts and microgreens as valuable components of a healthy diet. This will foster consumer confidence and support the continued growth of these foods.

Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic composition on cellular antioxidant activity and antimalarial property  

Amanda dos Santos Lima, Thiago Mendanha Cruz, Alessandra Oliveira Silva, Tanya Cox, Júlia Vitória Barbosa Dias, Jaqueline de Araújo Bezerra, Marcos dos Santos Lima, Marcell Crispim, Luciana Azevedo, Marciane Magnani, Daniel Granato

DOI: 10.1016/j.fufo.2025.100650

This study assessed the effect of simulated human digestion on the phytochemical properties and antioxidant capacity of Amazonian pataua (Oenocarpus bataua) pulp extract in human cells and plasma, and the antimalarial effect against Plasmodium falciparum. The composition of the pulp extract before and after in vitro digestion was analysed by liquid chromatography. Eleven polyphenols were identified and quantified, where (+)-catechin, vanillic acid and isorhamnetin were the primary compounds. The initial screening of the water-ethanol extract (50:50 v/v) showed cytotoxicity against normal cells after digestion, justified by increased intracellular reactive oxygen species (ROS). When subjected to an in vitro digestion process, the extract’s oxygen radical absorbance capacity, iron-reducing capacity, and human plasma protection were adversely decreased. However, undigested and digested pataua pulp extracts could reduce intracellular reactive oxygen species generation in red blood cells. Moreover, both undigested and digested pataua pulp extracts showed antimalarial activity against Plasmodium falciparum, with the digested extract being more promising due to its higher potency and greater selectivity, as indicated by a lower IC50 and higher selectivity index. This study provides a solid foundation for rationally exploiting pataua as a novel source of biologically active compounds.

Impact of Wine Polyphenols on the Inflammatory Profile of Induced Apical Periodontitis in Rats.

Rafaela Ricci, Bharbara de Moura Pereira, Julissa Denisse Arguello Alvarado, Romulo de Oliveira Sales-Junior, Nathália Evelyn da Silva Machado, Doany Cevada Dos Santos, Felipe Haddad Martim Pederro, Marciane Magnani, Marcos Dos Santos Lima, Edilson Ervolino, Luciano Tavares Ângelo Cintra, Anil Kishen, João Eduardo Gomes-Filho

DOI:  10.1016/j.joen.2025.01.023

Introduction: This study evaluated the impact of dealcoholized red wine polyphenols on the inflammation and lesion volume associated with apical periodontitis (AP) in rats. Methods: Thirty-two Wistar rats receiving AP induction were arranged as follows: Control Group, Dealcoholized Red Wine Group (DRW), Red Wine Group, and Alcohol Group (ALC). Solutions were administered daily in a volume of 4.28 mL/kg via gavage for 45 days. Mandibles and maxillae were removed for histologic, immunohistochemical (IL-1β, IL-10, tumor necrosis factor-alpha, receptor activator of nuclear factor κB ligand, osteoprotegerin [OPG], and tartrate-resistant acid phosphatase), and micro-computed tomography analyses of the AP site. A statistical analysis was performed with a significance level of 5%. Results: Inflammation and TRAP-positive cell count were similar for DRW and Red Wine Group, but lower when compared to Control Group and ALC (P < .001). The immunohistochemical expression of OPG was higher for DRW than for ALC (P < .05). A larger lesion volume was observed in ALC compared to other groups (P < .001). Conclusions: Prophylactic administration of dealcoholized red wine significantly reduced inflammation, decreased the number of TRAP-positive cells, enhanced OPG expression, and reduced lesion volume compared to water and alcohol solutions.

β-Glucan Alone or Combined with Lactobacillus acidophilus Positively Influences the Bacterial Diversity and Metabolites in the Colonic Microbiota of Type II Diabetic Patients.

Jéssika Rodrigues Clementino, Louise Iara Gomes de Oliveira, Mateus Kawata Salgaço, Fellipe Lopes de Oliveira, Victoria Mesa, Josean Fechine Tavares, Ludmilla Silva-Pereira, Breno Vilas Boas Raimundo, Karen Cristina Oliveira, Alexandra Ivo Medeiros, Francyeli Araújo Silva, Katia Sivieri, Marciane Magnani

DOI: 10.1007/s12602-025-10491-9

β-Glucan is a fermentable polysaccharide with prebiotic properties that has been shown to improve metabolic indicators. This study evaluated the effects of spent brewer’s yeast β-glucan (BGL) and Lactobacillus acidophilus LA-5 (106 CFU/g) (LA5) alone and in combination (LA5-BGL) on the composition of the fecal microbiome of adults with type 2 diabetes mellitus (T2DM) using the Human Gut Microbial Ecosystem Simulator (SHIME®). Short-chain fatty acids (SCFAs), ammonium ions, and cytokines (IL-6 and IL-10) were measured. BGL, LA5, and LA5-BGL increased (p < 0.05) the richness and diversity of microbial communities in the gut microbiome of individuals with T2DM. All treatments increased (p < 0.05) the abundance of Bacteroides, Alistipes, Lactobacillus, Subdoligranulum, and Acidaminococcus, along with increased (p < 0.05) production of SCFAs and anti-inflammatory cytokine (IL-10) compared to the control group. BGL treatments showed a greater increase in microbial diversity, SCFAs levels (butyric, propionic, and acetic acid), and the anti-inflammatory cytokine (IL-10). LA5 showed the highest decrease in ammonium ion levels. Results indicate that BGL may have a prebiotic and immunomodulatory effect on the fecal microbial community and metabolic indicators in adults with type 2 diabetes mellitus (T2DM). Findings underscore the role of BGL as a prebiotic food.

Preferred Attribute Elicitation (PAE) in the Sensory Descriptive Analysis of Foods: A Deep Comprehensive Review of the Method Steps, Application, Challenges, and Trends.

Izabeli Batista Girarducci da Silva, Marciane Magnani, Erick Almeida Esmerino, Elson Rogerio Tavares Filho, Adriano Gomes Cruz, Tatiana Colombo Pimentel

DOI: 10.1111/1541-4337.70197

Descriptive analysis (DA) is the gold standard for sensory profiling due to its robustness and reliability. However, its high cost, time demand, and labor intensity limit routine applications in the food industry. Rapid Sensory Profiling Techniques (RSPTs) have emerged as faster, less resource-intensive alternatives to address these challenges. Among them, preferred attribute elicitation (PAE) stands out. This review aims, for the first time, to comprehensively clarify applications of PAE in food products and provide insights into challenges and trends. PAE enables consumers to collaboratively identify, measure, categorize, and prioritize key attributes while providing hedonic insight. By ranking attributes based on their relevance to product acceptance and preference, PAE integrates descriptive and affective dimensions of sensory perception, enhancing the understanding of consumer experience and product appeal. PAE has been applied to fruits, meat, dairy, dairy alternatives, bakery products, and beverages, yielding sensory profiles comparable to those obtained via DA. It has also been used to evaluate processing, resampling, storage, and fermentation effects, distinguish commercial products, and analyze sensory perception differences among consumer groups. Key methodological factors affecting PAE’s reliability include the moderator’s expertise, panelist profile, and attribute selection. Future research should expand its application to unexplored food categories, validate its relevance compared to traditional acceptance tests, assess its effectiveness in complex food matrices, and integrate it with other sensory methods. This is the first comprehensive review of the PAE application, offering practical insights for researchers and the food industry on correctly using this method.

Meropenem-Induced Cross-Protection in Salmonella enterica Resistant Variants: Insights from Adaptive Laboratory Evolution and Whole Genome Sequencing

Ruthchelly Tavares da Silva, Ivo García-Penas, Laura Espina, Diego García-Gonzalo, Marciane Magnani, Rafael Pagán

DOI: 10.1016/j.fm.2025.104823

Adaptive laboratory evolution (ALE) assay provides a deep understanding of the genetic mechanisms and processes involved in bacterial responses. This study aimed to obtain resistant-variants (RVs) of Salmonella enterica subsp. enterica Typhimurium (SeT) and Enteritidis (SeE) after ALE assays with meropenem (MPM); to identify genetic modifications of RVs responsible for their increased resistance; and to evaluate their cross-protection against antibiotics and food preservatives (heat and carvacrol). Five MPM-RVs from SeT (SeT_M1-5) and one from SeE (SeE_M5) showed a 100 % increase in MPM minimum inhibitory concentrations (from 0.0312 to 0.0625 μg/mL). MPM-RVs had lower maximum growth rates and/or longer lag times, except in SeT_M5 and SeE_M3, which maintained or even improved growth fitness in the presence of MPM. Whole genome sequencing of RVs revealed single mutations in AMR-related and -unrelated genes in most MPM-RVs: spoT in SeT_M1, glnA in SeT_M2, thrS in SeT_M4 and SeE_M2, and mrdA in SeT_M5, SeE_M3 and SeE_M5. Two mutations were identified in SeT_M3 and SeE_M4: spoT and znuA in SeT_M3 and AWJ12_RS14025 and rfbF in SeE_M4. The individual mutations resulted in cross-protection to at least one of the antibiotics tested and/or heat (reductions of up to 3.8-4.3 cycles of inactivation for MPM-RVs in contrast to 4.7 and 5.3 cycles for SeE and SeT, respectively) and/or carvacrol (3.4-3.7 cycles for MPM-RVs in contrast to 4.4 cycles for SeT). These findings highlight the importance of preventing the emergence of resistant bacterial variants, considering the cross-protection to other antibiotics and food preservatives, which plays an important role in spreading resistance in food chain.

Probiotics and Prebiotics in Foodborne Illness: Mechanisms, Applications, and Future Directions

Louise Iara Gomes de Oliveira, Ana Rita Ribeiro de Araujo, Tatiana Colombo Pimentel, Vittorio Capozzi, Taliana Kenia Alves Bezerra, Marciane Magnani

DOI: 10.1016/j.jfp.2025.100584

Foodborne diseases (FBDs) represent a global public health concern, with millions of reported annual cases resulting in significant outbreaks, hospitalizations, and deaths. In this context, probiotics and prebiotics emerge among the promising alternatives to antibiotics. This mini-review aims to analyze the available evidence, mechanisms, and perspectives on the possible role of probiotics and prebiotics in preventing or treating FBDs. Probiotics have demonstrated effectiveness against various pathogens by producing antimicrobial substances such as fatty acids, organic acids, volatile compounds, and bacteriocins, resulting in immune system regulation, reducing the viable number of these microorganisms, and eliminating or neutralizing their toxins. Furthermore, they may offer health benefits through the positive modulation of the gut microbiota, strengthening immunity, and reducing pathogen colonization. Prebiotics, in turn, act as substrates promoting the growth of probiotics and increasing the production of desired metabolites, such as short-chain fatty acids (SCFAs). SCFAs support probiotics in competing with pathogens, particularly for adhesion sites on intestinal cells. Prebiotics can also have direct antimicrobial action, controlling pathogen adhesion and modulating the immune system by stimulating cytokine production and improving intestinal barrier integrity. Although previous reviews have discussed the general benefits of probiotics and prebiotics, this mini-review offers a more in-depth analysis of their beneficial effects against FBDs. It also explores the isolated and combined potential of probiotics and prebiotics as therapeutic alternatives and adjuvants to classical antibiotic therapies in preventing and controlling foodborne diseases, pointing out the mechanisms that could help to improve global public health.

Mechanisms of emerging technologies for inactivating foodborne viruses

Ruthchelly Tavares da Silva, Yago Alves de Aguiar Bernardo, Louise Iara Gomes de Oliveira, Carlos Adam Conte-Junior, Marciane Magnani

DOI:10.1128/aem.00242-25

Contamination of food and water by viruses is a major public health issue worldwide. Several viruses are associated with foodborne outbreaks, with norovirus and hepatitis A virus being the primary causes of foodborne outbreaks, followed by hepatitis E virus and rotavirus. These viruses are responsible for the majority of outbreaks reported globally, representing a significant challenge to food safety; therefore, effective viral inactivation processes are needed. This review presents and discusses recent research involving emerging technologies used for the inactivation of foodborne viruses, emphasizing the mechanisms involved in the process, their effectiveness, and the main challenges associated with the application. Traditional methods, like heat treatments (pasteurization) and sanitizers (organic acids), are effective but have drawbacks, such as the nutritional and sensory losses of food. Novel technologies to overcome the limitations of thermal treatments and guarantee food safety have been developed, such as UV C light (UV-C), cold plasma, high-pressure processing (HPP), and ultrasound. These methods have been shown to be effective in inactivating viruses in fresh foods (fruits, vegetables, and seafood), beverages, and food-contact surfaces, without compromising food properties. Among these technologies, HPP and UV-C were the most studied. HPP compromises the structural integrity of the virus, while UV-C induces photochemical damage to viral DNA and RNA. These alterations, combined with other physical and chemical effects, contribute to the destruction of viral genetic material, leading to viral inactivation. Despite their effectiveness, non-thermal technologies still face barriers, such as strict regulations and high costs, which limit their widespread application.

Valorization of Spondias bahiensis waste: Ultrasound-assisted extraction, in vitro phenolics bioaccessibility, and effect on the stability of lipid-protein oxidation in pre-cooked goat meatballs.

Valquíria Cardoso da Silva Ferreira, Deocleciano Cassiano de Santana Neto, Miriane Moreira Fernandes Santos, Anna Júllia Bezerra da Silva, Gleyce Swellen de Azevedo Moura, José Evangelista Santos Ribeiro, Marcos dos Santos Lima, Marciane Magnani, Fábio Anderson Pereira da Silva

DOI: 10.1016/j.fbio.2025.106792

This study aimed to optimize the ultrasound-assisted extraction of phenolic compounds from the bagasse of umbu-cajá (Spondias bahiensis), a native Brazilian fruit, and to assess their bioaccessibility. Additionally, the influence of different concentrations of the umbu-cajá bagasse (SBB) extract on the oxidative stability of pre-cooked goat meatballs was investigated over a 10-day refrigerated storage period by quantifying lipid oxidation products. A central composite rotational design determined the optimal ethanol concentration, sonication time, and bagasse-solvent ratio. The optimized extract was applied to meatballs at two concentrations: EX2 (meatballs with 7.0 g/100 g of added SBB extract) and EX4 (meatballs with 13.7 g/100 g of added SBB extract). A control treatment (no antioxidant) and BHT-treated meatballs (0.02 g/100 g) were included as references. Meatballs were evaluated by measuring instrumental color, peroxide value, TBARS, carbonyl compounds, and through simulated digestion. Optimal extraction conditions were 55 % ethanol, 20 min of sonication, and a 1:36.8 bagasse-to-solvent ratio (m/v). The extract showed high antioxidant activity and bioaccessibility of catechin (1243.75 %), epicatechin gallate (918.75 %), procyanidins B2 (915.38 %), gallic acid (319.76 %) and procyanidins B1 (136.36 %). SBB extract slowed lipid oxidation until day 10 and protein oxidation until day 5 but did not inhibit color change. The meatballs had low content of oxidative compounds and high antioxidant capacity in the intestinal fraction, indicating that SBB extract can partially or totally replace the synthetic preservative.

Açaı́ Pulp Treatment in a Continuous-Loop Ultrasonic Reactor: Effect on Enzymatic Activity, Color, and Bioaccessibility of Phenolic Compounds

• Dantas, Aline Macedo Batista, Jéssica Dayanne Ferreira dos Santos Lima, Marcos Fernandes, Fabiano A. N. Rodrigues, Sueli  Magnani, Marciane Borges, Graciele da Silva Campelo 

DOI:  10.1021/acsfoodscitech.4c00970

ABSTRACT: Açaı́pulp is known for its rich content of bioactive compounds. This study aimed to explore the effects of a continuous-loop ultrasonic system on the enzymatic activity, phenolic profile, antioxidant activity, color, and bioaccessibility of acaı́ pulp to improve processing and potential health benefits. The main operating parameters of a continuous-loop reactor (residence time and ultrasonic power density) were evaluated by applying an experimental design. The continuous processing increased the flavonoid content (452 mg of GAE/100 g). Enzyme activity was inhibited by 42% for peroxidase and 62% for polyphenol oxidase. Lower enzyme activity and higher anthocyanin content (10.94 mg of C3GE/100 g) contributed to the intensification of the color. Treatments performed at 2700 W/L for 50 s and at 1800 W/L for 100 s increased the bioaccessibility of some phenolic compounds, with a consequent increase in antioxidant activity. The use of the continuous-loop ultrasonic system resulted in a high-quality product due to the short processing times and improved the nutritional and sensory quality of açaı́pulp. 

Salting-out liquid-liquid extraction (SALLE) of phenolic compounds of fruits and ultra-fast RP-HPLC/DAD analysis: Validation of the method and characterization of fruits from the Brazilian Caatinga biome

Larissa Monteiro Ana Júlia de Brito Araújo Carvalho Marciane Magnani Marcos Dos Santos Lima

DOI: 10.1016/j.jfca.2025.108214

Phenolic compounds are widely investigated fruit components due to their benefits to human health, making robust, high-throughput methods necessary for their evaluation. In this study, an ultra-fast method for determining phenolic compounds by RP-HPLC/DAD, combined with the salting-out dispersive liquid-liquid extraction (SALLE) technique, was validated and applied to the characterization of eleven fruit species endemic to the Caatinga, a biome unique to Brazil. The SALLE fruit matrix extraction technique proved to be suitable. The method allowed for the analysis of 40 phenolics in 13.6 minutes, performing equivalently to UHPLC systems, with adequate validation parameters: linearity (R² ≥ 0.999), precision (CV% ≤ 6.88%), recovery (80.1% – 110%), and LOD ≤ 0.967 mg/L and LOQ ≤ 3.224 mg/L. A total of 35 phenolics were quantified in the endemic fruits of the Caatinga and showed a high phenolic richness in the genus Spondias, such as ‘seriguela’ and ‘umbu-cajá,’ as well as ‘pitanga’ and passion fruit P. cincinnata. The compounds that stood out were procyanidin A2, quercetin 3-glucoside, epicatechin, chlorogenic acid, rutin, naringin, hesperidin, and caffeic acid.

A robust method for quantifying 42 phenolic compounds by RP-HPLC/DAD: Columns performance and characterization of Brazilian Citrus peels

Marcos dos Santos Lima,Larissa Iris da Silva Monteiro,Ana Júlia de Brito Araújo Carvalho,Débora Costa Bastos,Tatiana Colombo Pimentel,Marciane Magnani  

DOI:10.1016/j.foodchem.2024.140807

Abstract

Reliable analytical methods are the basis for the elucidation of phenolic compounds in foods. This study aimed to optimize and validate a method for determining 42 phenolics using reverse-phase (RP) high-performance liquid chromatography (HPLC) coupled to diode-array-detector-DAD. The performance of two RP columns was evaluated. The 150×4.6 mm 3-μm column showed superior separation quality, whereas 35 of the 42 phenolics showed a separation resolution ≥1.5. The method’s linearity, precision (coefficient variation< 3.09%), recovery (87.5-103.2%), specificity, limits of detection (0.04-0.25 mg/L), and quantification (0.06-0.25 mg/L) had acceptable ranges. Thirty phenolics were quantified in Citrus peels, mainly flavanones, flavanols, flavonols, and phenolic acids, highlighting the high values of hesperidin (535-35070 mg/kg) and naringin (26-36466 mg/kg). Lemon peels named ‘Lisboa,’ ‘Thaiti,’ ‘Thaiti-2000’, and ‘Thaiti-2001’ presented the main phenolics associated with antioxidant capacity. The presented method was robust for determining 42 phenolic compounds, offering a new approach for

Milkfat influences thermal tolerance and biofilm formation of Salmonella Typhimurium during pasteurization

Ziqi Liu, Yuechong Liu, Jian He, Xiaoyan Pei, Yixin Lin, Marciane Magnani, Donghong Liu, Tian Ding, Jinsong Feng 

 DOI: 10.1016/j.foodres.2025.117538

Abstract

The bacterial contamination and failure of pasteurization are leading threats to the safety of dairy productions. Milk contains a large quantity of fat which supports bacterial growth but its influence on pasteurization efficacy and biofilm formation remains poorly understood. In this study, we first demonstrate that the inactivation of Salmonella during pasteurization is significantly less effective in whole milk compared to skim milk, with the D-value increasing from 0.60 min in skim milk to 0.67 min in whole milk (P = 0.0047). In whole milk, Salmonella biofilms form greater biomass and display a distinct architecture from biofilms in skim milk. Specifically, Salmonella in whole milk was embedded within the extracellular polymeric substances (EPS), while cells in skim milk biofilms were sparsely distributed. Raman spectroscopy showed that biofilms formed in whole milk possess a distinctly higher lipid content. Therefore, we hypothesized that the thermal tolerance of Salmonella in whole milk was directly linked to its physical interaction with milkfat. Confocal laser scanning microscopy showed an increase from 26.1 % to 86.1 % of the fraction of Salmonella co-localizing with milkfat after 12 h of incubation in whole milk. Lipase treatment (200 units/mL) on whole milk reduced biofilm formation and improved the efficacy of thermal inactivation. Our findings demonstrate the milkfat acts as a physical scaffold, facilitating bacterial aggregation and the formation of a protective, lipid-rich biofilm matrix enhancing Salmonella survival during pasteurization. These insights should inform pasteurization strategies of high-fat dairy products to ensure food safety.

Development of Optimized Origanum vulgare L. Essential Oil-Loaded Chitosan/Gum Arabic Nanocapsules by Complex Coacervation

Sonálle C A Andrade , Ana F C Uchôa , Allessya L D Formiga , Anny L M R Cardoso , Nereide S S Magalhães , Rodrigo O França, Sócrates G Dos Santos, Marciane Magnani, Francisco H Xavier-Junior , Thayza C M Stamford 

DOI: 10.1021/acsomega.5c09153

Abstract

Oregano essential oil (OEO), rich in carvacrol and thymol, has bioactive properties but is prone to degradation due to its volatile nature. Nanoencapsulation by complex coacervation, using chitosan (CHI) and gum arabic acid (GA), emerges as an alternative to increase its stability in food matrices. This study investigated the influence of the CHI/GA mass ratio on the formation of OEO-containing nanocapsules and quantified the encapsulated carvacrol using validated GC-MS. A Box-Behnken experimental design optimized the OEO concentration, CHI/GA ratio, and amount of Tween 80. Physicochemical properties such as the diameter, PdI, and zeta potential were evaluated. Morphology was analyzed by SEM and AFM, and thermal stability by TGA and DSC. Stability was monitored for 120 days at 4 °C, 25 °C, and 40 °C. The optimized formulation (470 mg of OEO, 659 mg of CHI/GA 1:5, and 13 mg of Tween) resulted in nanocapsules with a diameter of 323 ± 22 nm, a PdI of 0.20 ± 0.02, and a zeta potential of +15.8 ± 0.8 mV. FTIR analysis confirmed electrostatic interactions between CHI and GA. GC-MS identified 24 constituents in the OEO, with carvacrol as the main compound (78.8%). The validated method showed an R ² of 0.9976, proving to be specific, precise, and accurate. The encapsulation efficiency was 95% ± 0.7, indicating that the technique preserved the oil’s composition and concentration. The nanocapsules maintained stability under different temperatures with confirmed structural integrity. It is concluded that nanoencapsulation via complex coacervation, combined with experimental design, allows for the production of stable and effective nanocapsules for the delivery of lipophilic bioactive compounds. The validated analytical method reinforces the system’s applicability in future research and the development of functional products.

Cassava (Manihot esculenta) bagasse as a fiber- and oligosaccharide-rich yeast protectant for enhancing chemical, functional, and sensory properties of cashew nut milk

Tânia Somera, Débora Pinhatari Ferreira, Carlos Eduardo Barão, Marcos Dos Santos Lima, Andresa Carla Feihrmann, Ísis Meireles Mafaldo, Tahis Regina Baú, Osvaldo Valarini Junior, Giselle Nobre Costa, Marciane Magnani, Tatiana Colombo Pimentel

DOI: 10.1016/j.foodchem.2025.146925

Non-Saccharomyces yeasts offer functional benefits to plant-based foods, but maintaining their viability is challenging. This study provides the first comprehensive characterization of cassava bagasse (CB) and evaluates its use as a protective matrix for Issatchenkia terricola 129 and Hanseniaspora opuntiae 125 during freeze-drying, 90-day refrigerated storage, simulated gastrointestinal conditions (SGIC) and incorporation into cashew nut milk, compared to fructooligosaccharides (FOS). CB exhibited a complex composition of fibers, oligosaccharides, amino acids, and minerals. It caused slightly greater viability loss immediately post-freeze-drying, but yeast survival during storage and SGIC was similar to FOS. In cashew nut milk, CB-yeasts showed improved growth and post-SGIC viability, along with enhanced organic acid production, viscosity, phenolic and fatty acid profiles and bioaccessibility, antioxidant and antidiabetic activities, and sensory properties. These findings demonstrate that CB is a sustainable and effective protectant that preserves yeast viability and improves the chemical and functional quality of plant-based beverages.

Chemistry Composition and Bioaccessibility Study of Phenolic Compounds and Carotenoids of New Brazilian Biofortified Sweet Potato “CIP BRS Nuti”

Maria Carla Cândido da Silva, Larissa Iris da Silva Monteiro, Darlanna de Souza Prado, Ana Júlia de Brito Araújo Carvalho, Marciane Magnani, Alexandre Furtado Silveira Mello, Erbs Cintra de Souza Gomes, Marcos dos Santos Lima 

DOI:10.1002/fci2.70041

Sweet potatoes biofortified in carotenoids have been an alternative to combat malnutrition in vulnerable populations. This work is the first comprehensive study of the bioactive potential of the new Brazilian biofortified sweet potato cultivar “CIP BRS Nuti.” In two harvests, the chemical composition and profiles of organic acids, phenolic compounds, and carotenoids were analyzed using high-performance liquid chromatography. The bioaccessibility of phenolics and carotenes was also studied. The main simple sugars and organic acids found were glucose, fructose, citric, malic, succinic, and lactic acids. “CIP BRS Nuti” showed high concentrations of chlorogenic acid (228.3–330.4 mg/kg) and hesperidin (385.2–530.9 mg/kg), with the presence of other phenolics such as catechin, epicatechin, and procyanidin B1, with high levels of bioaccessibility (> 104%). The main carotenoid quantified was 9-cis-β-carotene (294.9–503.8 mg/kg), with low bioaccessibility, but β-carotene and lycopene were also present. Our results show the nutritional and bioactive potential of the “CIP BRS Nuti” potato, providing essential information for its use in food and industrialization, with an applied view to nutrition and food science. 

Assessment of the effectiveness of irradiation in inactivating or reducing levels of Salmonella, E. coli and L. monocytogenes in fruits and vegetables: A systematic literature review

Oluwadara Alegbeleye ^{a 1}, Maria Paula MBB Gonçalves ^{a 1}, Oluwafemi Bamidele Daramola ^b, Beatriz Manfrinato Ferreira ^a, Aruni Narmada Athukorala ^c, Bamikole Peter Akinde ^d, Marciane Magnani ^e, Anderson S. Sant’Ana

DOI:10.1016/j.foohum.2025.100865 

Abstract

Enteric pathogens pose significant food safety risks, particularly in fruits and vegetables, which are typically consumed raw or only minimally processed. Irradiation technologies are one of several viable intervention strategies shown to enhance the microbiological safety of fresh produce by effectively reducing levels of or eliminating these pathogens. This review is a synthesis of 43 peer-reviewed studies that assessed the effectiveness of irradiation technologies in inactivating selected foodborne pathogens (L. monocytogenes, Salmonella spp., and Escherichia coli) on/in fruits and vegetables. A systematic literature search to cull studies that investigated the application of irradiation technologies on fresh produce was conducted. Data were extracted regarding the types of irradiation technologies used, the pathogen(s) targeted, doses applied, and the resulting effects on microbial populations. The review identified that though effectiveness can vary based on the type of produce, the specific pathogens, and the irradiation doses applied, irradiation technologies (0.2–7.0 kGy), including X-rays, gamma rays, electron beams and light irradiation, significantly reduce levels of L. monocytogenes, Salmonella spp., and E. coli on fruits and vegetables. The review also identifies gaps in sensory and nutrient retention assessments and highlights the need to optimize irradiation doses to balance microbial safety and produce quality. The findings of this systematic literature review can potentially inform research priorities and regulatory guidance for more efficient adoption of irradiation in fresh produce safety management.

Sourdough bread making: autochthonous lactic acid bacteria to optimize fermentation and enhance functional properties

Tatiana Zanella Rodrigues, Ingrid Conceiçao Dantas Gonçalves, Ana Regina Simplício de Medeiros, Maria Laysa Cirilo Barnabe, Gabriel Victor Pinheiro Barbosa, Patricia Pinheiro Fernandes Vieira, Jaielison Yandro Pereira da Silva, Rene Pinto da Silva, Marcos dos Santos Lima, Angela Maria Tribuzy de Magalhães Cordeiro, Evandro Leite de Souza, Maria Elieidy Gomes de Oliveira, Estefânia Fernandes Garcia 

DOI: 10.1016/j.ijgfs.2025.101315

The increasing demand for healthier food options and lifestyles has intensified interest in fermented products such as sourdough bread, recognized for their potential health benefits and bioactive compounds. This study aimed to develop sourdough (S) and sourdough bread (SB) inoculated with lactic acid bacteria (LAB) isolated from Brazilian sourdough. Three bread formulations were prepared with different fermentation times (2, 3, and 4 h), using sourdough inoculated with Companilactobacillus paralimentarius 41 (Cp41), Lactiplantibacillus plantarum 47 (Lp47), and Levilactobacillus brevis 83 (Lb83), along with a non-inoculated control. The effects of fermentation time and bacterial strain on bread quality (physicochemical and microbiological parameters), functional properties, in vitro protein and starch digestibility (hydrolysis index and estimated glycemic index) were evaluated. Sourdough and bread dough inoculated with LAB exhibited improved yeast adaptation and higher LAB counts at the end of fermentation. The Lp47-inoculated samples demonstrated the highest production of exopolysaccharides (EPS). SBLp47 and SBLb83 fermented for 4 h presented higher acidity and the greatest in vitro protein digestibility. Among all samples, SBLb83 fermented for 3 h exhibited the lowest estimated glycemic index (73.78 ± 0.91). SBLp47 and SBLb83 showed increased lactic acid concentrations and reduced levels of fructose and glucose as a result of fermentation. After 3 and 4 h of fermentation, SBLb83 demonstrated the highest phenolic compound content. LAB-inoculated sourdough bread exhibited superior antioxidant activity compared to the control across all fermentation periods. Improvements in antioxidant activity, phenolic content, glycemic response, and protein digestibility were observed in sourdough breads inoculated with Lp47 and Lb83, highlighting their potential as promising starter cultures. A fermentation period of 3 h was identified as the most favorable condition. 

Adherence to dietary practices recommended by the Dietary Guidelines for the Brazilian Population among people with obesity: baseline of a community trial carried out at the Health Fitness Program in Belo Horizonte, Brazil, 2022-2023 

Mariana Souza Lopes, Vitória Marli Serafim Moreira Silva, Nathália Luíza Ferreira, Maria Cecília Ramos de Carvalho, Patrícia Pinheiro de Freitas, Aline Cristine Souza Lopes 

DOI: 10.1590/S2237-96222024v34e20240287.pt 

Objective: To analyze sociodemographic and health factors associated with high levels of adherence to dietary practices recommended by the Dietary Guidelines for the Brazilian Population among people with obesity taking part in the Health Fitness Program in Belo Horizonte, Minas Gerais, Brazil. Methods: This is a cross-sectional study with a representative sample of Health Fitness Program participants with obesity who were ≥20 years old. Sociodemographic and health data were collected, and the adherence to the Dietary Guidelines scale was assessed, classified as: low (<32 points), medium (32-41 points) and high (>41 points). Multivariate logistic regression was performed. Results: In all, 1,109 individuals participated, the majority of whom were women (92.5%) and had at least one chronic disease (85.8%). The majority self-rated their health status as very good/good (62.2%), 62.8% had hypertension and 68.0% had Class 1 Obesity. 46.0% had good levels of adherence to the Dietary Guidelines. After adjustment, high levels of adherence were associated with being elderly (odds ratio [OR] 1.47; 95%CI 1.1; 2.0), having higher income (OR 0.58; 95%CI 0.41; 0.81) and better self-rated health (regular: OR 0.73; 95%CI 0.55; 0.96; poor/very poor: OR 0.26; 95%CI 0.13; 0.52). Conclusion: High levels of adherence to the Dietary Guidelines were associated with stage of life, better income and positive self-rated health, highlighting the need to target efforts towards adults and vulnerable groups.

Qualificação profissional à distância para promoção da alimentação adequada e saudável: fatores associados à evasão 

Mariana Souza Lopes, Izabela Santana Sathler, Cheila de Sousa Jardim Soares, Nathália Luíza Ferreira, Aline Cristine Souza Lopes 

DOI: 10.1590/S2237-96222025v34e20240338.pt 

Objetivo: Analisar os fatores associados à evasão de qualificação profissional à distância para promoção da alimentação adequada e saudável no Brasil. Métodos: Trata-se de estudo transversal com profissionais do Sistema Único de Saúde (SUS) matriculados em curso à distância, ofertado entre 2019 e 2020, pautado em materiais do Ministério da Saúde. Avaliaram-se dados sociodemográficos e profissionais associados à evasão, utilizando regressão logística múltipla (p-valor<0,05). Resultados: A prevalência de evasão foi 30,6%. A evasão se associou a residir nas macrorregiões Norte (p-valor 0,009) e Nordeste (p-valor<0,001), ser enfermeiro (p-valor<0,001), possuir menores níveis de formação (graduação: p-valor 0,004; especialização/residência: p-valor 0,025), ter maior tempo de atuação no SUS (p-valor 0,004), ter realizado o curso por meio de celular ou tablet (p-valor 0,024), ter qualificação prévia sobre alimentação adequada e saudável (p-valor 0,007) e não ter utilizado anteriormente os materiais do curso (p-valor 0,021). Conclusão: A evasão se associou a residir entre os enfermeiros, profissionais das macroregiões Norte e Nordeste, com menor nível de formação, maior tempo de atuação no SUS, e entre aqueles que acessaram o curso principalmente por celular ou tablet, que haviam participado previamente de qualificação profissional sobre a temática e que não haviam utilizado os materiais empregados no curso. A modificação desses fatores requer apoio institucional, com integração dos diferentes entes federados, visando superar os desafios locais enfrentados pelos profissionais para a conclusão de atividades de educação permanente. 

“Desrotulando” application: Brazilian consumer support tool for healthy food choices 

Michele de Carvalho Andrade; Mariana Souza Lopes; Aline Cristine Souza Lopes; Bruna Vieira de Lima Costa.

DOI: 10.1590/1413-81232026313.10032024 

The study aimed to describe an application to support Brazilian consumers, focusing on the motivation for users to join the application and the profile of the most researched foods. It was a descriptive study with secondary data from the application obtained from September 2022 to January 2023. The data analyzed includes the category of packaged foods, degree of industrial processing according to the NOVA classification, healthiness score generated by the application, and users’ motivations for joining. A total of 116,594 new users were registered in the application, and 1,286,114 foods were scanned. Health (42.4%) and weight control (37.2%) stood out as motivations for joining. The prevalence of foods scanned was ultra-processed (52.4%), followed by minimally processed foods (21.9%). As for the healthiness score, most of the scanned foods had good grades. Ultra-processed foods presented a score distribution with significant variability, with a predominance of lower scores. The wide use of the application shows its ease of use and importance as a tool to support Brazilian consumers in making healthier food choices. 

The Results of Ultra-Processed Food Consumption on Weight Change: A Randomized Controlled Community Trial in a Health Promotion Program

Mariana Souza Lopes, Patrícia Pinheiro de Freitas, Aline Cristine Souza Lopes 

 DOI: 10.3390/nu17040638

Objective: Our objective was to evaluate the association between ultra-processed food (UPF) consumption and body weight change after participating in nutritional intervention.

Design: Our study was a 12-month follow-up of participants in a randomized controlled community trial.

Setting: Brazilian Primary Health Care.

Participants: The participants were health promotion services users. Users in the control group (CG) performed the service’s usual intervention, while those in the intervention group (IG) additionally participated for seven months in nutritional intervention. Socioeconomic data, self-health, perception of time spent in health promotion services, and weight loss attempts were investigated. Food consumption was obtained by 24 h food recall and categorizing these in quartiles according to the Nova system of food classification. Weight was measured and changes in the 12-month period were calculated by subtracting the weight at follow-up from the baseline measurement.

Results: Of the participants, 88.1% were females aged 56.7 ± 11.8 with 19.7 ± 15.3 months of participation in the service. In the fourth quartile (highest UPF consumption), the % contribution of calories per consumption of UPFs was 47.7%, with no differences between the IG and CG (p = 0.406). Adjusted after 12 months, when comparing those with lower consumption of UPFs (first quartile), individuals from the second, third, and fourth quartiles had positive weight variation. Respectively, these variations were as follows: 0.363 kg (95% CI: 0.038; 0.689; p = 0.029); 0.467 kg (95% CI: 0.159; 0.776; p = 0.003); and 0.389 kg (95% CI: 0.061; 0.717; p = 0.020, with no differences between IG and CG).

Conclusions: The percentage contribution of calories from UPFs was associated with positive weight change, which contributes to the growing evidence of the relationship between UPFs and obesity.

Neighborhood Vulnerability and the Consumer Food Environment in an Urban Area

Cecilia Craveiro, Mariana Lopes, Patricia Freitas, Aline Lopes

 DOI: 10.3390/ijerph22020303

Abstract

The consumer food environment is an important medium for understanding complex interactions regarding food consumption, health outcomes and social vulnerability. We aimed to analyze the diversity, variety and quality of natural and ultra-processed foods in a Brazilian metropolis. We performed a cross-sectional study, analyzing food stores within a buffer (1600 m) area around 18 randomly selected Health Promotion Program units. We used descriptive analyses and regression models, adjusted by the area’s population, to examine associations of consumer food environment variables with the health vulnerability (HVI) of the territory and store type. Low HVI areas had higher fruits and vegetables variety adequacy and better quality, when compared to medium and high/very high HVI areas (p-value < 0.001 and p-value = 0.001). Supermarkets in low HVI areas had almost twice the prevalence of adequate vegetable variety (65.2% vs. 33.3% in high/very high HVI areas, p-value = 0.005). Adjusted by population, areas with high/very high HVI had lower odds of adequate fruit variety when compared to low HVI areas (OR = 0.06; CI 95% = 0.01-0.44; p-value = 0.006). Although consumer preference is important in food acquisition, disparities in quality, diversity and variety within the consumer food environment could lead to difficulties in access to healthy options for vulnerable populations.

Access to fruits and vegetables according to health vulnerability and food insecurity in primary care

Luiza Ali Dinis, Mariana Souza Lopes, Denise Sales, Aline Cristine Souza Lopes, Patrícia Pinheiro de Freitas

DOI: 10.1186/s41110-025-00377-9

Verifying the association between the prevalence of commercial fruit and vegetable establishments and food insecurity (FI) in the territory of the Health Academy Program (HAP) from Belo Horizonte, Minas Gerais, Brazil. Ecologic study. Eighteen units were classified by the Health Vulnerability Index (HVI). The establishments that trade fruit and vegetable included in a 500 m buffer around the participants household were analyzed. The insecurity was investigated by the Brazilian Food Insecurity Scale. 44.9% of the HAP units analyzed were classified as high/extremely high HVI. 29.4% were in FI situation, with a superior prevalence in the territory with high/extremely high HVI. Near these units, a lower preponderance of specialized fruit and vegetable market and open-air food market (50. 5% vs. 66.0%) and large chain markets and supermarkets (18.5% vs. 21.8%), when compared to the units with medium HVI. We identified a higher preponderance of specialized market and open-air food market (65.2% vs. 50.0%) and large chain markets and supermarkets (21.3% vs. 20.0%) in the territories with a lower prevalence of FI. The results showed the need for public policies that could reduce the inequities in access to healthy food, seeking to support actions to promote adequate and healthy food developed by the HAP. 

Community Food Environment in Brazilian Medium-Sized Municipality After the Ore Dam Break: Database Creation and Diagnosis

Patrícia Pinheiro de Freitas, Mariana Souza Lopes, Nathália Luíza Ferreira, Sérgio Viana Peixoto, Aline Cristine Souza Lopes

DOI: 10.3390/ijerph22111723

This study proposed a methodology for obtaining a valid database of food retail establishments and characterized the community food environment, understood as the distribution and type of food outlets, in a Brazilian medium-sized municipality after the collapse of a mining tailings dam. An ecological study was conducted with establishments selling food for home consumption (butcher shops, fish markets; fruit and vegetable specialty markets; large- and small-chain supermarkets; bakeries and local markets) and immediate consumption (bars, snack bars, and restaurants). For home-consumption establishments, data were requested from governments and completed with website/app searches, virtual audits (Google Street View), and on-site audits. For immediate-consumption establishments, only on-site audit was used due to the low quality of the secondary databases. Agreement between databases was assessed with the Kappa statistic. Density (d) was calculated by the area (in km²) of the sampling stratum. Public databases presented low validity (23.0%; Kappa -0.388; p = 1.000), even after virtual auditing (31.4%; Kappa 0.37; p < 0.001). 96 establishments for home consumption and 261 for immediate consumption were identified, with predominance of local markets (35.4%), bars (35.2%), and snack bars (29.1%). The region with the highest density of establishments was the “Other Areas” stratum (d = 4.7 for home-consumption establishments and d = 13.2 for immediate-consumption establishments). Audit proved most effective, especially for small establishments. The lack of governmental databases and the identified food environment should inform municipal policies to promote food and nutrition security and reduce inequalities after the disaster.

 Análise do impacto ambiental das refeições escolares no Litoral da Paraíba: uma avaliação das pegadas de carbono, hídrica e ecológica

Theo Meira de Farias, Giovanna Carolina Weizel Lacouth, Jéssica Bezerra dos Santos Rodrigues, Ingrid Conceição Dantas Gonçalves, Noádia Priscila Araújo Rodrigues

DOI: 10.20396/san.v31i00.8677001 

Introduction: Food systems are one of the main contributors to global environmental impacts, including greenhouse gas emissions, excessive water use, and soil degradation. Objective: Given this scenario, the objective of this study was to analyze the environmental impact of foods served in school meals in public schools in municipalities along the coast of Paraíba, Brazil, using sustainability indicators such as carbon, water, and ecological footprints. Methods: The research was based on secondary data from the bidding processes for food procurement under the National School Feeding Program (PNAE) in 2022, and focused on quantifying the per capita impacts associated with the foods purchased for school meals. Results: The results indicated that the food groups “Meat and Offal” and “Poultry and Eggs” had the highest environmental impacts, with the largest values in carbon, water, and ecological footprints. In contrast, plant-based foods, such as legumes, cereals, and vegetables, showed lower impacts. Discussion: Thus, the results suggest that reducing the consumption of meat and processed foods, along with increasing plant-based and minimally processed options, could significantly reduce the environmental impact of school meals while contributing to the food and nutritional security of students. Conclusion: Therefore, adopting more sustainable dietary practices, aligned with PNAE guidelines in menu planning, is essential to promote student health and environmental preservation, and to enhance public food and nutrition policies, contributing to the Sustainable Development Goals (SDGs). 

Long-Term Effects of Food Insecurity on Body Mass Index and Weight of Adults: A Cohort in a Region in Northeast Brazil

Maria das Graças Diniz Alves, Larissa Ferreira Tavares Nonato, Renatha Celiana da Silva Brito, Poliana De Araújo Palmeira 

DOI: 10.1080/19320248.2025.2462052 

This study analyzed the effects of food insecurity (FI) on Body Mass Index (BMI) and body weight in a cohort of individuals living in Brazil. Included 214 individuals followed in 2011, 2014, and 2019. The Brazilian Household Food Insecurity Measurement Scale estimated the FI considering levels of severity. Multilevel mixed-effects linear regression models were conducted. The body weight and BMI increase were associated with Mild FI and Moderate FI over time. People who live with obesity may be facing a chronic experience of lack of access to food, so it is essential to adopt and intersectoral public policies. 

Rede de proteção ao direito humano à alimentação adequada e à insegurança alimentar: uma coorte no semiárido nordestino 

Ana Beatriz Macêdo Venâncio dos Santos; Poliana de Araújo Palmeira; Angelo Giuseppe Roncalli da Costa Oliveira 

DOI: 10.1590/1413-81232025307.01882024 

Resumo O estudo analisou o tempo de convivência com a insegurança alimentar (IA) e o acesso aos programas governamentais, identificando a rede de proteção ao direito humano à alimentação adequada (DHAA) acessada por famílias do semiárido brasileiro entre 2011 e 2019. Trata-se de uma coorte realizada em Cuité, Paraíba, com coletas em 2011, 2014 e 2019. Analisou-se o tempo de convivência com a IA, com classificação em: segurança alimentar persistente, IA em um tempo, IA em dois tempos e IA persistente, a partir da definição de segurança e insegurança da Escala Brasileira de Insegurança Alimentar. Verificou-se mudança no acesso a 25 programas e nas características sociodemográficas das famílias com o teste Q de Cochran e a associação entre o acesso e o tempo de convivência com a IA por meio do teste qui-quadrado de Pearson. Observou-se a coexistência da IA crônica, da vulnerabilidade sociodemográfica persistente e de uma rede de programas diversa e pouco acessada. O fortalecimento dessa rede deve visar transformações estruturais mediante um plano de desenvolvimento socioeconômico que promova a igualdade de oportunidades e articule economia e bem-estar social. 

Long-term effect of food insecurity on body weight gain and metabolic risk in a context of high socioeconomic vulnerability in a medium-income country: the SANCuité cohort, Brazil, 2011-2022

Jackson Silva Lima Laurentino, Isadora Macedo de Oliveira Martins-Costa, Rônisson Thomas de Oliveira-Silva, Ana Beatriz Macêdo Venâncio Dos Santos, Poliana de Araújo Palmeira 

DOI: 10.3389/fpubh.2025.1574499

Objective: Using longitudinal data from a study conducted in an area of high socioeconomic vulnerability in Brazil, we examined the long-term effects of food insecurity (FI) and social determinants on body weight gain (body weight, BMI) and metabolic risk (waist circumference – WC, waist-to-height ratio – WHtR) over 11 years of follow-up, conducted between 2011 and 2022.

Methods: Face-to-face household interviews were conducted using the Brazilian Food Insecurity Scale to measure FI, and anthropometric measurements of weight, height, and WC were taken. Data analysis was performed in STATA 15.0 using multilevel mixed-effects regression with covariate adjustment and predicted marginal means with marginal differences.

Results: Among the 210 individuals followed over 11 years, high prevalence of FI was observed (2011: 51.8%; 2022: 45.9%), central adiposity (2011: 83.8%; 2022: 88.6%), as well as a significant increase in the prevalence of high BMI (2011-2022: +16.7 p < 0.00), general obesity (2011-2022: +15.2 p < 0.00), and abdominal obesity (2011-2022: +0.5 p 0.02) over time. Multivariate analysis showed a positive association between BMI and body weight with mild, moderate, and severe FI after 8 and 11 years of follow-up among adults. A progressive increase in predicted body weight and BMI scores was observed among adults, with an increase of +5.6 (p 0.02) and + 2.3 (p 0.01) at the end of the follow-up, respectively, being higher in individuals with severe FI compared to those with food security. Among people ≥60 years old, WC and WHtR mean varied according to time and FI categories, with a positive association observed in mild and moderate FI, and an inverse association with severe FI at the end of the follow-up.

Conclusion: FI is a risk factor for long-term weight gain and obesity, particularly in vulnerable populations, highlighting the need for intersectoral public policies to ensure food and nutrition security, combat obesity, and combat the structural causes of poverty and FI.

Association between Food Insecurity and Overweight: a Systematic Review based on Intersectionality of Gender and Race/Color

Renatha C S Brito, Poliana A Palmeira, Jackson S L Laurentino, Rônisson T Oliveira-Silva, Ana B M V Santos, Angelo G R C Oliveira

DOI: 10.1093/nutrit/nuaf152

Context: The relationship between Food Insecurity (FI) and health conditions such as chronic diseases, including obesity, has been discussed in the literature. However, discourse on the association of FI with other social vulnerabilities is a relatively new and less elucidated area.

Objective: Considering the evidence that gender and race/color are factors of interest in the relationship between FI and overweight, in this study we aimed to carry out a systematic review to analyze the state of knowledge about this relationship and how the intersectionality of these factors permeates the association of FI and overweight in the Brazilian population.

Data sources: We performed this review according to the methodological criteria recommended by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Four electronic databases were used to search for articles reporting observational studies in Brazil published between 2003 and 2023. Eligible articles reported studies that evaluated the association between nutritional status and FI based on the Brazilian Food Insecurity Scale (Escala Brasileira de Insegurança Alimentar [EBIA]).

Data extraction: We used the most relevant terms frequently observed in the literature to locate the most significant possible number of studies related to the topic. Articles were selected using prespecified inclusion criteria. Four independent researchers carried out data screening.

Data analysis: Initially, the search resulted in 568 articles. After review of the articles found in the initial search, 22 articles were included in the review, of which 45% (n = 10) reported studies that showed a statistically significant association between FI and overweight. In 5 of these articles the studies also showed an association with gender, and 4 studies showed an association with gender and race/color.

Conclusion: Black women with low income, limited educational levels, and lack of social security and protection revealed a high vulnerability to overweight and its comorbidities. There is an urgent need for inclusive strategies that consider not only the physical dimension but also the social, historical, and cultural factors that permeate the scenario of obesity in women and the Black population, based on deeper understanding of the theoretical basis for these findings that culminates in the formulation of more effective and equitable policies.

Addressing food insecurity in early childhood programs through a health equity lens: A qualitative case study of Brazil’s Criança Feliz program

Gabriela Buccini, Poliana de Araújo Palmeira, Ana Poblacion, Muriel Bauermann Gubert 

DOI: 10.1371/journal.pone.0329310

Abstract

Background: Food insecurity negatively impacts early childhood health and development. Recommendations for establishing equitable actions to address food insecurity in early childhood programming have not been developed globally. The Brazilian Criança Feliz Program (PCF), one of the largest early childhood programs worldwide, has faced implementation challenges while addressing food insecurity. We aimed to (i) understand how food insecurity affects the PCF’s internal (i.e., organizational-level factors) and external (i.e., family-level and system-level factors) implementation contexts, and (ii) develop equity-focused recommendations for early childhood programs to mitigate food insecurity.

Methods: Qualitative case study analyses of in-depth interviews with PCF implementation teams and families in five Brazilian municipalities. Participants shared their experience with PCF implementation quality, including questions related to food insecurity. A three-stage rapid qualitative approach was used: (a) inductive thematic analysis identified central codes related to food insecurity, (b) deductive approach to organize central codes within themes related to internal and external contexts of PCF operations, and (c) integration of findings into a set of equity-focused recommendations based on the four categories of the Getting to Equity (GTE) framework.

Results: 240 interviews were conducted. Internal programmatic barriers included lack of protocols for screening, referring, and following up with families struggling to access food as well as challenges to engage them in early learning activities. External programmatic barriers included family-level factors (e.g., unrealistic support expected from PCF) and system-level factors (e.g., bureaucracy in accessing safety nets). Ten equity-focused recommendations across GTE framework focused on improving program curriculum and protocols to mitigate food insecurity and increasing individual and community capacity.

Conclusions: We documented barriers at the family, program, and system levels to address food insecurity in the Brazilian PCF home visiting program. Barriers informed the generation of equity-focused programming recommendations to improve practices to address food insecurity, not only for the PCF, but also for the global community implementing home visiting programs.

Social Determinants of the Transition in Food Consumption in Paraíba, Brazil, Between 2008 and 2018

Sara Ferreira de Oliveira, Rodrigo Pinheiro de Toledo Vianna, Poliana de Araújo Palmeira, Flávia Emília Leite de Lima Ferreira, Patrícia Vasconcelos Leitão Moreira, Adélia da Costa Pereira de Arruda Neta, Nadjeanny Ingrid Galdino Gomes, Eufrásio de Andrade Lima Neto and Rafaela Lira Formiga Cavalcanti de Lima

DOI:10.3390/nu17152550

Abstract

Background/Objectives: Dietary patterns have changed over time, characterising a process of nutritional transition that reflects socioeconomic and demographic inequalities among different populations. This study assessed changes in dietary consumption patterns and the associated social determinants, comparing two time periods in a sample of individuals from a state in the Northeast Region of Brazil. Methods: Data from the 2008–2009 and 2017–2018 Household Budget Survey for the state of Paraíba were analysed, totalling 951 and 1456 individuals, respectively. Foods were categorised according to the NOVA classification and compared based on sociodemographic and economic variables. To determine the factors that most strongly explain the contribution of each NOVA food group to the diet, beta regression analysis was conducted. Results: Differences were observed between the two periods regarding the dietary contribution of the NOVA food groups, with a decrease in consumption of unprocessed foods and an increase in ultra-processed foods. Living in urban areas, being an adolescent, and having an income above the minimum wage were associated with reduced intake of unprocessed foods in both periods. Additionally, being an adolescent and having more than eight years of schooling were associated with higher consumption of ultra-processed foods. Conclusions: The population under study showed changes in food consumption, reflecting a transition process that is occurring unevenly across socioeconomic and demographic groups, thereby reinforcing social inequalities. These findings can guide priorities in food and nutrition policies, highlighting the need for intervention studies to evaluate the effectiveness of such actions.

Climate change, food insecurity, and the impacts on child health and nutrition in Brazil: proposal for a conceptual model 

Lissandra Amorim Santos-Degner, Poliana de Araújo Palmeira, Elisabetta Gioconda Iole Giovanna Recine, Elaine Martins Pasquim, Rosana Salles-Costa, Ana Maria Segall-Corrêa, Janaína Braga de Paiva, Larissa Ferreira Tavares Nonato, Sandra Maria Chaves dos Santos 

DOI: 10.1590/0102-311XPT217824 

As mudanças climáticas vêm causando maior frequência e intensidade dos eventos climáticos extremos, levando cerca de 72 milhões de pessoas em todo o mundo a enfrentar limitações no acesso aos alimentos em 2023. No Brasil, esse cenário agrava as situações de fome crônica, pobreza e desigualdades sociais, sobretudo, aumentando a vulnerabilidade em crianças menores. Este estudo tem por objetivo propor um modelo conceitual que explicite e explique estas relações. Foi realizada pesquisa bibliográfica com os termos “mudança climática”, “insegurança alimentar” e “má nutrição infantil”, e a combinação entre eles com a inclusão da palavra “Brasil”, no sentido de discutir o modelo no contexto da realidade brasileira. Foi proposto modelo conceitual que apresenta as relações entre os elementos principais e outros três de mediação e/ou complexidade – sistema alimentar, insegurança hídrica e desigualdades sociais – que se relacionam por cinco vias: (a) impacto direto dos eventos climáticos extremos sobre o acesso ao alimento; (b) impacto dos eventos climáticos extremos no acesso ao alimento, em decorrência dos seus efeitos no sistema alimentar; (c) insegurança hídrica como elemento que adiciona complexidade à relação dos extremos com a insegurança alimentar; (d) as desigualdades sociais como determinantes do efeito das mudanças climáticas sobre os domicílios em condição de insegurança alimentar, baixo acesso à água e/ou má nutrição infantil; (e) a saúde e nutrição infantil afetada por todos esses fatores. As conexões discutidas no modelo poderão orientar futuras pesquisas, favorecendo o desenvolvimento e a implementação de ações colaborativas e multissetoriais de adaptação e construção de resiliência às mudanças climáticas no Brasil. 

Individual and environmental factors affect the consumption of ultra-processed foods among Brazilian adolescents: results from the National School Health Survey

Maria Eduarda da Costa Andrade, Rodrigo Pinheiro de Toledo Vianna, Diôgo Vale, Poliana de Araújo Palmeira 

DOI: 10.1017/S0007114525105898

This population-based cross-sectional study investigated the complex interplay of factors influencing high ultra-processed food (UPF) consumption among Brazilian adolescents using a hierarchical socio-ecological model. Data from 100 028 adolescents (13-17 years) enrolled in public and private schools nationwide were collected via self-administered questionnaires from the 2019 National School Health Survey. High UPF consumption was defined as ≥ 7 subgroups consumed on the previous day based on the NOVA classification. Poisson regression adjusted for complex sampling and hierarchical structure identified prevalence ratios (PR) for associated factors. High UPF consumption was significantly associated with younger age (PR = 1·22; 95 % CI 1·11, 1·34), regular breakfast consumption (PR = 1·32; 95 % CI 1·23, 1·42), regular screen time during meals (PR = 1·36; 95 % CI 1·27, 1·45), frequent UPF purchases at and around school (PR for canteen: 1·57; 95 % CI 1·43, 1·72; street vendors: 1·71; 95 % CI 1·55, 1·89), higher maternal education (PR 1·23, 95 % CI 1·12, 1·36) and lower parental supervision (PR 1·34, 95 % CI 1·11, 1·62). Living in the South (PR 1·50, 95 % CI 1·34, 1·69), Southeast (PR 1·30, 95 % CI 1·17, 1·44) and Midwest regions (PR 1·21, 95 % CI 1·09, 1·34) also correlated with higher consumption. Conversely, high body satisfaction and attending private school showed an inverse association. These findings underscore the intricate, multilevel influences on UPF consumption among Brazilian adolescents. Integrated interventions, spanning schools, family environments and public policies are crucial for promoting healthier eating habits and preventing obesity in this vulnerable population.

Food insecurity, gender and race/color: a study in the semi-arid northeast

Thaís da Conceição Pereira; Elisiandre Martins de Lima; Rafaela Lira Formiga Cavalcanti de Lima; Rosana Salles-Costa; Poliana de Araújo Palmeira 

DOI: 10.1590/1413-81232025307.02022024 

This study aimed to analyze the association between food insecurity (FI) and the gender and race/color characteristics of the reference people in households in a municipality in the semiarid northeastern region. A cross- -sectional study was conducted, with a probabilistic sample and household interviews, with 737 households in 2022- 2023. The Brazilian Food Insecurity Scale was used to measure FI, and self-declared gender and race/color profiles were created (White man, White woman, Black man, Black woman). Multivariate multinomial logistic regression models were estimated to analyze the association between AI profiles and levels. A high prevalence of FI (41.6%) was observed, especially in households where the reference person was a woman. Households headed by black women and white women were more likely to be in Moderate/severe FI when compared to white men. The findings suggest that gender and race/skin color should be included in formulating and implementing public policies related to AI in an intersectional and intersectoral manner, especially in populations that present more significant social and climatic vulnerabilities, such as the northeastern semiarid region. 

Exclusive breastfeeding: does the presence of a partner have a positive impact on its duration? cohort study 

Laís Kisly Costa Silva, Maria Augusta Correa Barroso Magno Viana, Sara Ferreira de Oliveira, Rafaela Lira Formiga Cavalcanti de Lima, Rodrigo Pinheiro de Toledo Vianna 

DOI: 10.1590/1806-9304202500000135-en 

Objectives: to identify whether the presence of a partner is a protective factor for a longer duration of exclusive breastfeeding (EBF) of women living in João Pessoa and assisted by the Public Health System. Methods: cohort with pregnant women recruited from health services and followed up at their homes. Socioeconomic and demographic information, information on prenatal care, childbirth, puerperium care and baby nutrition were collected. Median EBF was calculated by survival analysis, Kaplan-Meyer, with log-rank test for group comparison. The independent EBF factors were estimated with multiple Cox regression. A 5% significance level was considered. Results: 162 women and their children were assessed. The median EBF was 120 days. Until the first four months of postpartum, women with a partner had longer EBF (p=0.002). In the final model, “having received prenatal guidance” (HR= 1.67; 95%CI= 1.05 – 2.65), “not having difficulty in breastfeeding after hospital discharge” (HR= 1. 58; 95%CI= 1.03 – 2.42) and “not using a pacifier” (HR= 1.64; 95%CI= 1.07 – 2.53) favored longer EBF duration. Conclusion: encouraging partner’s support for longer periods may be a viable strategy for improving EBF. Promotional actions and not offering pacifiers are important for a longer duration of EBF, and support and management in the puerperium period needs to be strengthened.

Avaliação do impacto da insegurança alimentar na saúde de mulheres gestantes e não gestantes: aspectos metodológicos de uma coorte no

município de João Pessoa

Maria Augusta Correa Barroso Magno Viana, Caio César Ferreira Alverga, Flora Luiza Pereira do Nascimento Freire, Raiane Fernandes de Azevedo Cruz, Rafaela Lira Formiga Cavalcanti de Lima, Rodrigo Pinheiro de Toledo Vianna

DOI: 10.20396/san.v31i00.8674724

Introdução:

A insegurança alimentar é um fenômeno complexo que pode causar repercussões na vida das pessoas,

mas sua avaliação, frequentemente baseada em estudos transversais, limita a análise de suas consequências causais

Objetivo:

Descrever  aspectos  metodológicos  de  um  estudo  longitudinal  prospectivo  sobre  o  impacto  da

insegurança alimentar na saúde de gestantes/puérperas, seus filhos e mulheres não gestantes.

Métodos:

Foram construídas duas coortes abertas: uma de gestantes/puérpe

ras e outra de mulheres não gestantes, recrutadas em

Unidades  de  Saúde  da  Família  (USF)  de  João  Pessoa,  entre  outubro  de  2018  e  agosto  de  2020.  Foi  avaliada  a

situação de insegurança alimentar familiar utilizando a Escala Brasileira de Insegurança Alimentar (EBIA), estado de ansiedade com Inventario de Ansiedade Traço

–

Estado, qualidade de vida com a escala WHOQoL versão breve,

avaliação de atividade física com a escala IPAQ versão curta, avaliação do consumo alimentar com recordatório

24 horas e antropometria seguindo recomendações do Ministério da Saúde.

Resultados:

Foram recrutadas 402 mulheres,  com  quatro  entrevistas  realizadas  em  intervalos  de  cerca  de  3  meses:  uma  na  USF,  duas  visitas

domiciliares e uma por telefone. Participaram 270, 212 e 110 mulheres

nas entrevistas subsequentes, sendo a última restrita  às  puérperas.  O  estudo  enfrentou  desafios  como  dificuldades  na  retenção  de  participantes  e  ajustes

logísticos  devido  à  pandemia,  mas  garantiu  a  integridade  do  protocolo.

Conclusão:

O  estudo  demonstrou alternativas  viáveis  para  pesquisas  de  coorte,  superando  desafios  e  assegurando  qualidade  metodológica.  Os resultados contribuirão para compreender as relações causais entre insegurança alimentar e desfechos de saúde.

Food insecurity in the rural area of a municipality in the Brazilian semiarid region: prevalence and associated factors

Elisiandre Martins de Lima,Thaís da Conceição Pereira,Rafaela Lira Formiga Cavalcanti de Lima,Catarine Santos da Silva, Poliana Araújo Palmeira

DOI: 10.1080/19320248.2025.2536226

This study examined the prevalence of food insecurity related to food production, access to water, and government programs within a rural area of a municipality in the Brazil semiarid region. This was a cross-sectional study with 273 participating households. Data on socioeconomic characteristics, access to social programs, and food insecurity were collected. Multiple logistic regression models were used to assess the associations between food insecurity and the variables studied. Food security was observed in 49.5% of the population. Food insecure families (55.0%) had greater access to government programs, showing that the programs are adequately targeted at families experiencing greater food vulnerability. 

O EFEITO DA QUALIDADE DE VIDA PARA A SAÚDE MENTAL DE GESTANTES EM SITUAÇÃO DE INSEGURANÇA ALIMENTAR

Maria Augusta Correa Barroso Magno Viana, Hugo Ramiro Melgar Quiñonez, Rafaela Lira Formiga Cavalcante de Lima, Nadjeanny Ingrid Galdino Gomes, Caio César Ferreira Alverga, Renata Pozelli Sabio, Rodrigo Pinheiro de Toledo Vianna

DOI: 10.16891/2317-434X.v13.e3.a2025.id2676 

O sofrimento mental está associado ao acesso inadequado a alimentos, e essa relação pode ser afetada pela qualidade de vida. O objetivo do estudo foi analisar a influência da qualidade de vida na relação entre insegurança alimentar e ansiedade entre gestantes residentes no município de João Pessoa, Paraíba. Trata-se de um estudo transversal realizado com uma amostra de 271 gestantes recrutadas em Unidades de Saúde da Família durante consultas de pré-natal de rotina. Além das características sociodemográficas, foram avaliadas a insegurança alimentar familiar, por meio da Escala Brasileira de Insegurança Alimentar, o risco de ansiedade, utilizando o Inventário de Ansiedade Traço-Estado, e a qualidade de vida, por meio do instrumento WHOQOL-breve da Organização Mundial da Saúde. As associações entre as variáveis do estudo foram testadas utilizando o teste qui-quadrado, regressão logística múltipla binária, análise multivariada de rede e análise de mediação. A insegurança alimentar afetou 45,4% das participantes do estudo, e 56,1% foram classificadas com risco de ansiedade. A insegurança alimentar aumentou em 2,2 vezes o risco de ansiedade (OR: 2,22; IC 95%: 1,29 – 3,81). A análise de rede mostrou a complexa relação entre as variáveis do estudo. O modelo múltiplo de mediação identificou que o domínio relações sociais da qualidade de vida apresentou um efeito de mediação de 25,2% (β= -0,77) na relação diretada insegurança alimentar com o risco de ansiedade. Os resultados evidenciaram que as redes de apoio social têm potencial para reduzir o impacto negativo da insegurança alimentar no risco de ansiedade de gestantes. 

Violence Against Lesbian, Gay, and Bisexual Older Adults: Findings From the Brazilian Health System

Daniel Canavese, Maurício Polidoro, Sávio Marcelino Gomes, Ariadne Ribeiro Ferreira

DOI: 10.1177/2752535X251327537

The primary objective of this research was to describe the prevalence of violence against older adults according to sexual orientation as documented in the Notifiable Diseases Information System (SINAN) in Brazil. SINAN is a national database that compiles violence-related notifications reported by healthcare services. This ecological study analyzed aggregated data at the population level, focusing on violence notifications involving individuals aged 50 and above from 2017 to 2021. The data underwent descriptive and bivariate statistical analyses, adhering to a significance level of 5%. The completeness percentage of the sexual orientation variable ranged from 70% to 72%. Our analysis detected noteworthy disparities in the distribution of violence notifications based on sexual orientation. Specifically, lesbians had the highest incidence rates of physical violence compared to heterosexual and bisexual women. In a broader context, women demonstrated a higher susceptibility to psychological violence, with bisexual women experiencing a notably elevated prevalence (41.67%). Reports of physical and psychological violence differed based on contextual variables, such as race, education level, and geographic region. We hereby conclude that the disparities found in violence notifications underscore a troubling situation faced by older adults within the LGB community, with a particular emphasis on the challenges encountered by bisexual individuals. Improving cultural competence in health systems is essential to enhancing data collection and understanding the complexities of violence against this population.

Disparity in meat consumption: An obstacle to achieving the golden rule of the Brazilian Dietary Guidelines?

Mariana Souza Lopes, Paulo Gustavo Costa e Silva Cruz, Joerika Batista Ciqueira, Cecília Furtado Craveiro, Izabele da Silva Rocha, Savio Marcelino Gomes

DOI: 10.1590/S2237-96222025v34e20240355.en 

Objective:

To investigate disparities in meat consumption in Brazil and analyze its relationship with the intake of fresh fruits and vegetables.

Methods:

This is a cross-sectional study using secondary data from the 2019 National Health Survey. Red meat consumption (in days) was estimated according to socioeconomic and demographic data. Odds ratios (OR) were estimated using multinomial logistic regression, and the association between meat consumption and fruit and vegetable intake was assessed by means of Poisson regression.

Results:

Of a total of 83,085 Brazilians participating in the study, 29.2% reported consuming meat 5-7 times per week. Sociodemographic and economic factors revealed a lower likelihood of consuming meat 5-7 days/week among women [OR 0.50, 95%CI 0.46; 0.53), older adults (OR 0.48, 95%CI 0.37; 0.63) and residents of the Northeast region of the country (OR 0.72, 95%CI 0.66; 0.79). After adjustments, red meat consumption was directly associated (p-value<0.001) with vegetable intake (0.04) and inversely associated with fruit intake (-0.04).

Conclusion:

Meat consumption is unequal among Brazilians and may have an impact on the consumption of fresh foods, such as fruits.

COVID‑19 pandemic exacerbated food insecurity in South American countries

Carlos Frederico A. Vasconcelos Neto, Michelle Jacob, Daniel Tregidgo, Denis Valle, Hani R. El Bizri, Sávio Marcelino Gomes, Julia E. Fa, Thais Q. Morcatty, Frederico Ozanan Barros Monteiro, Alessandra Scofield, Alessandra Matte, Willandia A. Chaves, Luiz Henrique Medeiros Borges, Antônia I. A. Silva, Dídac Santos‑Fita, Tiago Lucena Silva, Isaac Ibernon Lopes‑Filho, Maria Isabel Afonso Silva, Rebeca Mascarenhas Fonseca Barreto, Marcela A Oliveira, Felipe Silva Ferreira, Ricardo Rodrigues Santos, Jaime Honorato‑Júnior, Marilene Vasconcelos Silva Brazil, Shirliane Araújo Sousa, Deise C. L. Oliveira, Valéria R. F. Ferreira, Hyago K. L. Soares, Marcia F. Pinto, Raone Beltrão‑Mendes, Marcos Paulo Lopes Rodrigues, Wáldima Alves Rocha, Roberto Gutiérrez Poblete, Francisco Luigi Schettini, Joe S. S. Rojas, Marco A. Aspilcueta, Justo D. V. Zevallos, Giussepe Gagliardi‑Urrutia, Erick Rodolfo Menéndez Delgado, Mariela Lissette Polit‑Vera, Elvira Rodríguez Ríos, Juan Carlos Carrascal Velásquez, Maria Dalila Forlano Riera, Lucy Perera Romero, Danilo A. Salas Dueñas, Daniel Garin, Pedro Mayor.

DOI: 10.1007/s12571-025-01538-4

The COVID-19 pandemic triggered unparalleled political, economic, and social ramifications, exacerbating global food insecurity (FI). To understand the overall impact of the pandemic and how different socio-economic groups were affected, we assessed prevalence and severity of FI in a sample of 18,997 households across seven countries in South America. We employed the Food Insecurity Experience Scale developed by the FAO. Our results showed that pre-pandemic, 4.5% of the sampled population across the entire continent faced Moderate FI, while 0.6% experienced severe FI. During the pandemic, Moderate FI increased to 16.9% (+ 12.4%), and Severe FI to 2.7% (+ 2.1%). By country, pre-pandemic households in Venezuela had the highest prevalence of Moderate FI (9.7%), with Peru experiencing the highest Severe FI frequency (1.1%). Peru had the greatest rise in Moderate (+ 23.9%) and Severe FI (+ 4.6%) during the pandemic. Low-income households, defined as those earning < 2 minimum wages per month, were most susceptible to FI. Uruguayan low-income families exhibited the most significant rise (+ 40.4%) in Moderate FI, while those in Peru experienced an increase of + 9.1% in Severe FI. This study measures the profound and far-reaching impact of the COVID-19 pandemic on FI in South America. Our findings also emphasise the critical importance of implementing effective public policy interventions to improve resilience against future shocks. This would enable policymakers to develop targeted strategies that address the immediate challenges posed by pandemics as well as laying the groundwork for a more resilient and sustainable food security landscape in the region.

Eating behavior of transgender people on hormone use

Renata Samali Dantas de Souza, Joana Sabino da Silva, Sávio Marcelino Gomes, Thaiz Mattos Sureira  

DOI: 10.12957/demetra.2025.84009 

Introduction: Hormone use among transgender individuals influences physical, biological, and psychological factors, which, in turn, affect eating behavior. Objective: To investigate eating behavior and characteristics of food craving, mindful eating, and food neophobia in transgender men and women. Methods: The study included 38 transgender individuals, 25 trans men and 13 trans women, all matched by age with cisgender individuals. Data collection was conducted online, and participants completed the Dutch Eating Behavior Questionnaire (DEBQ), the Food Craving Questionnaire-State (FCQ-S) and short Trait version (FCQ-T), the Mindful Eating Scale (MES), and the Food Neophobia Scale. Results: The eating behavior, food craving, mindful eating, and food neophobia of transgender individuals resembled both their sex assigned at birth and their gender identity. Similar patterns were observed in emotional eating, external eating, and dietary restraint, and the majority of transgender individuals (60.53%) showed neutrality regarding food neophobia. Conclusion: From this perspective, the findings suggest no significant differences in the influence of hormone use on the eating behavior of transgender individuals and their characteristics of food craving, mindful eating, and food neophobia. The results highlight the impact of social and psychological factors on the eating patterns of the studied population, as well as the importance of appropriate professional support, with an inclusive and competent approach, to ensure comprehensive care. 

Black women facing food insecurity are more vulnerable to the double burden of malnutrition in Brazil: evidence from Brazilian National Dietary Survey 

Sávio Marcelino Gomes, Aline Martins de Carvalho, Alisson Diego Machado, Eduardo De Carli, Mariana Alves Ferreira, Michelle Cristine Medeiros Jacob, Dirce Maria Lobo Marchioni

DOI: 10.1007/s12571-025-01607-8 

Malnutrition, in all its forms, is one of the leading global challenges. The intersection of gender and race-based oppression can negatively influence the burden of malnutrition. Likewise, nutritional outcomes can affect each other, as seen in the relationship between food insecurity and underweight or obesity. In this study, our aim was to understand how the impact of food insecurity on underweight, overweight, and obesity varies across gender and race categories. To achieve this, we used data from a nationally representative Brazilian nutritional survey and conducted a multinomial logistic regression model to estimate Odds Ratios and 95% Confidence Intervals. White women in food insecurity showed higher odds of developing obesity (1.40, CI95 1.10–1.77), while Black women simultaneously presented higher odds of presenting both underweight (1.41, CI95 1.00-1.98) and obesity (1.42, CI95 1.16–1.73), relatively White man in food security. We demonstrated that the effect of food insecurity on malnutrition, at a population level, can vary according to individuals’ gender and race. Thus, we conclude that promoting food and nutrition security could reduce gender and race disparities in nutritional outcomes.

Indirect influence of global climate change, mediated by nutrition, on anxiety disorders 

Ulysses Paulino Albuquerque, Rodrigo Felipe Rodrigues do Carmo, Sávio Marcelino Gomes, Reginaldo Augusto Farias de Gusmão, Michelle Cristine Medeiros Jacob, Arthur Ramalho, María Teresa Pulido Silva, Juliana Kelly da Silva-Maia, Nicola Schiel, Mauro Dias Silva Júnior, Antonio Souto,  Renata Adrielle Lima Vieira

DOI: 10.1007/s44187-025-00622-0

Global climate change has profound direct and indirect impacts on human health, including mental health disorders such as anxiety. In this study, we hypothesize the pathways through which climate change contributes to anxiety disorders, with a specific focus on the indirect effects mediated by nutrition. The direct pathway involves environmental stressors such as rising temperatures and extreme climate events, which elevate anxiety levels through chronic stress and uncertainty about the future. The indirect pathway is shaped by climate-induced food insecurity and other dietary and environmental changes, leading to nutritional deficiencies and gut microbiome alterations that disrupt neurological and physiological processes associated with anxiety. Climate change can reduce agricultural diversity and alter the nutritional composition of food, contributing to deficiencies in key micronutrients (e.g., zinc, iron, and B vitamins) essential for neurotransmitter regulation and brain function. Additionally, the increasing prevalence of ultra-processed foods exacerbates these issues. In contrast, climate-induced pathways, such as heat exposure, dehydration, altered food quality, and increased risks of infectious diseases, also affect the gut microbiome, further impacting mental health. There is a reciprocal influence between the gut microbiome and mental health, and a mediation effect of diet and climate-sensitive environmental factors on mental well-being, with disruptions in microbial diversity linked to higher anxiety risks. We propose an integrative framework that illustrates both pathways and highlights the urgency of policy interventions addressing climate-related changes in the food system to mitigate anxiety disorders. Although there is a complex phenomenon in which the policies and strategies of agrifood systems impact food insecurity, we specifically aim to discuss an overlooked aspect of this phenomenon here. We advocate that the understanding of climate change and nutrition is essential for developing effective public health strategies that incorporate environmental sustainability, food security, and mental health resilience. 

Body mass index and body composition changes in transgender people undergoing gender-affirming hormone therapy: a systematic review and meta-analysis

Ísis Gois, Felipe Behrends Rodrigues, Marcos Pereira, Magnus R Dias-da-Silva, Sávio Marcelino Gomes

DOI: 10.1007/s11154-025-09985-2

Gender-affirming hormone therapy (GAHT) uses sex steroid hormones to induce desired physical changes, improving the quality of life of transgender individuals. While generally considered safe, its effects on body composition and adipose tissue remain a topic of debate. The aim of this review was to verify whether GAHT is associated with weight gain and body composition in transgender individuals. This is a systematic review with meta-analysis (April-September 2022, updated March 2025) following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, including cohort, case‒control, and clinical trial studies from four databases. The outcomes analyzed included body mass index (BMI), body fat (BF), lean mass (LM), and waist circumference (WC). Among the 1,896 identified studies, 29 were included in the systematic review, and 28 were included in the meta-analysis. GAHT increased BMI in assigned male at birth (AMAB) individuals (0.55 kg/m², 95% CI: 0.14-0.97) and assigned female at birth (AFAB) individuals (0.92 kg/m², 95% CI: 0.55-1.29). The AMAB participants presented a reduction in LM (-1.81 kg, 95% CI: -3.15, -0.47) and an increase in BF (4.27 kg, 95% CI: 3.15-5.39), whereas the AFAB individuals presented an increase in LM (4.98 kg, 95% CI: 4.06-5.91) and a decrease in BF (-2.13 kg, 95% CI: -3.52, -0.75). No significant changes in WC were observed in either group. According to the GRADE assessment, the certainty of evidence was moderate for BMI and low to very low for changes in waist circumference, lean mass, and fat mass. GAHT is associated with increased BMI and body composition changes. These alterations align with the expected outcomes for transgender individuals and do not appear to be directly linked to cardiovascular risks.

Ginger beers fermented with fruits: Chemical and sensory characterization and effects on target colonic microbial groups of vegan adults

Louise Iara Gomes de Oliveira, Whyara Karoline Almeida da Costa, Fernanda de Candido de Oliveira, Marcos dos Santos Lima, Evandro Leite de Souza, Thatyane Mariano Rodrigues de Albuquerque, Roger Wagner, Tatiana Colombo Pimentel, Marciane Magnani 

DOI: 10.1016/j.fbio.2024.105636 

Berries (GB_B) and cajá (GB_C) ginger beers were assessed for the physicochemical parameters, metabolites, and sensory attributes during fermentation. Furthermore, their effects on the colonic microbiota of vegan adults were determined in 48 h in vitro fermentation. During fermentation, both GBs showed increases in the concentrations of phenolic and volatile compounds, as well as in alcohol levels. GB_B and GB_C can be classified as low-alcohol beverages, with ethanol contents of 0.61% and 0.72% after 7 days of fermentation, and 1.5% and 2.3% after 14 days, respectively. The profile of organic and phenolic acids depended on the type of fruit. Sensory attributes perceived by the panelists were mainly associated with specific volatile compounds, even when the concentrations were below the odor thresholds. GB_B and GB_C increased the relative abundance of Bifidobacterium at 24 h of colonic fermentation and the concentration of short-chain fatty acids, while decreasing Clostridium histolyticum and Eubacterium rectale/C. coccoides. Our results demonstrated that the ginger beers produced with berries and cajá exhibited a remarkable profile of bioactive compounds, potentially contributing to positive effects on human gut microbiota assessed in in vitro tests. 

Microencapsulated phenolic compounds from organic coffee husk: Impacts on human gut microbiota and in vitro prebiotic potential

Gezaildo Santos Silva, Flávia Izabely Nunes Moreira, Thatyane Mariano Rodrigues de Albuquerque, Thaianaly Leite Abreu, Eike Guilherme Torres de Souza, Layane Rosa da Silva, Antonia Dayane Jenyffer de Farias Marques, Mércia de Sousa Galvão, Marcos Dos Santos Lima, Evandro Leite de Souza, Marta Suely Madruga, Louise Emy Kurozawa, Taliana Kênia Alencar Bezerra

DOI: 10.1016/j.foodres.2024.115597

Abstract

This research investigated the influence of the microencapsulation of phenolic compounds (PCs) from organic coffee husk with whey protein concentrate (WPC) and maltodextrin on the abundance of intestinal bacterial populations and their metabolic activity during in vitro fecal fermentation. The microencapsulated PCs were gradually metabolized during fecal fermentation, resulting in significant transformations and an increase in PCs in the fermentation media. The metabolism of PCs by the fecal microbiota occurred concurrently with the consumption of sugars, production of organic acids, and reduction in pH in the media. The PCs, especially when encapsulated, promoted an increase in the abundance of Lactobacillus spp./Enterococcus spp., Bifidobacterium spp., and Ruminococcus albus/R. flavefaciens, and a reduction in the abundance of Bacteroides spp./Prevotella spp., Clostridium histolyticum, and Eubacterium rectale/Clostridium cocoides. The results suggest that the PCs exhibited prebiotic potential, with their efficacy enhanced by microencapsulation, particularly when WPC was used exclusively as the encapsulating agent.

Investigating the release of the bioactive components of novel nutraceuticals combining potentially probiotic Limosilactobacillus fermentum and freeze-dried jabuticaba peel during gastrointestinal digestion for long-term storage

Heloisa Maria Almeida do Nascimento, Thatyane Mariano Rodrigues de Albuquerque, Jaielison Yandro Pereira da Silva, Sônia Paula Alexandrino de Oliveira, Genilton Alves da Silva, Noádia Priscilla Araújo Rodrigues, José Elson Soares Filho, Maria Lúcia da Conceição, Mariana Costa Monteiro, Evandro Leite de Souza 

DOI: 10.1007/s12602-025-10614-2

This study investigated the release of bioactive components from novel nutraceuticals that combine potentially probiotic Limosilactobacillus fermentum and freeze-dried jabuticaba peel during gastrointestinal digestion for long-term storage. The bioaccessibility of phenolic compounds, antioxidant capacity, cell viability, and physiological status of L. fermentum in two nutraceuticals [mix of potentially probiotic L. fermentum + freeze-dried jabuticaba peel (FJP), termed JM); mix of potentially probiotic L. fermentum + FJP + fructooligosaccharides (FOS), termed JFM)] were assessed when exposed to a standardized simulated gastrointestinal digestion (SGD) during 90 days of storage (4 ± 0.5 °C, 11% relative humidity). JFM showed the highest viable cell counts of L. fermentum (3.76 ± 0.18-5.12 ± 0.12 log CFU/mL) after exposure to SGD during 90 days of storage. JFM presented the largest subpopulation of L. fermentum live cells (29.1 ± 0.66%) and the smallest subpopulation of L. fermentum dead cells (5.8 ± 0.48%) during SGD. The bioaccessibility of anthocyanins in JM and JFM was greater after gastric exposure, while the bioaccessibility of ellagic acid was greater after ileal exposure. The antioxidant capacity of JM and JFM was reduced during SGD and storage, likely due to the degradation of bioactive components. JM and JFM released metabolically active L. fermentum cells and bioaccessible phenolic compounds along the SGD. FOS could have led to JFM performing better overall than JM in the measured variables. The results demonstrate the efficacy of JM and JFM in maintaining their bioactive functionalities during gastrointestinal digestion, allowing them to reach the colonic environment, where they can induce changes in the intestinal microbiota and induce systemic beneficial health effects.

Chitosan-Gum Arabic Nanocapsules Loaded with Oregano Essential Oil for Microbial Control in Tuna Fillets

Sonalle Carolina Albuquerque de Andrade, Marciane Magnani, Tatiana Colombo Pimentel, Alessandra Silva Araújo, Nereide Stela Santos Magalhães, Whyara Karoline Almeida da Costa, Thatyane Mariano Rodrigues de Albuquerque, Francisco Humberto Xavier Junior, Thayza Christina Montenegro Stamford

DOI: 10.1021/acsfoodscitech.5c00478 

Tuna (Thunnus spp.) is a fish widely produced and consumed worldwide. This study characterized nanocapsules of Origanum vulgare L. essential oil (OVEO) with chitosan and gum Arabic (N-OVEO) and evaluated their antimicrobial activity in controlling Listeria monocytogenes and Staphylococcus aureus in bluefin tuna (Thunnus thynnus) fillets during storage. N-OVEO presented a smooth surface with a uniform and spherical format. The encapsulation efficiency of OVEO was 95%. The minimal inhibitory concentration (MIC) of N-OVEO was 10 and 20 μL/mL against L. monocytogenes and S. aureus, respectively. N-OVEO (MIC) reduced 2.6 log CFU/mL of S. aureus and 3.5 log CFU/mL of L. monocytogenes after 24 h of exposure. In tuna fillet, N-OVEO (MIC) reduced 2 and 2.4 log CFU/mL of S. aureus and L. monocytogenes, respectively. Overall, these findings support using N-OVEO as a promising strategy to guarantee the safety and biopreservation of fish. 

Submerged fermentation with autochthonous Pediococcus pentosaceus enhances physicochemical properties, enriches bioactive compounds, and boosts the antioxidant and antibacterial activities of mandacaru (Cereus jamacaru DC.) fruit

Gracy Kelly Vieira de Vasconcelos Medeiros, Ana Cristina Silveira Martins, Mateus Gomes Vasconcelos, Estefânia Fernandes Garcia, Noádia Priscila Araújo Rodrigues, Marcos Dos Santos Lima, Yuri Mangueira do Nascimento, Evandro Ferreira da Silva, Josean Fechine Tavares, Marcelo Sobral da Silva, Thatyane Mariano Rodrigues de Albuquerque, Vanessa Bordin Viera, Maria Lúcia da Conceição, Evandro Leite de Souza, Maria Elieidy Gomes de Oliveira

DOI: 10.1016/j.foodchem.2025.145947

This study aimed to measure the impacts of submerged fermentation using Pediococcus pentosaceus 57 (Pp-57) on the physicochemical properties, bioactive compounds, antioxidant, and antibacterial activities of mandacaru fruit (Cereus jamacaru DC.). The commercial strain Lactobacillus acidophilus La-05 was used as a control. After 96 h of fermentation, viable cell counts of lactic acid bacteria (LAB) were > 7 log CFU/mL. During fermentation, pH and total soluble solids decreased, while acidity, flavonoid content, total phenolic compounds, and antioxidant (DPPH) and antibacterial activities increased. In the ¹H NMR analysis, more than 20 compounds were identified in the mandacaru fruit before fermentation. After 72 h of fermentation, differences in the signals related to sugars, caprylic acid, acetoin, acetoacetic acid, and lactic acid were observed. The results show that submerged fermentation using LAB, particularly the native strain P. pentosaceus 57, may effectively boost the bioactive compound profile and the antioxidant and antibacterial properties of mandacaru fruit.

Lower Cortisol and Dehydroepiandrosterone Sulfate and Higher Food Addiction in Childhood Obesity: Associations With Stress and Dietary Parameters

Rúbia Cartaxo Squizato de Moraes, Thallyta Alanna Ferreira Viana, Joicy Karla Grangeiro Pereira, Paulo César Trindade da Costa, Davyson Barbosa Duarte, Lydiane de Lima Tavares Toscano, Manuel Francisco de Araújo Lima, Melyssa Kellyane Cavalcanti Galdino, Joelma Rodrigues de Souza, Francisco Antônio de Oliveira Júnior, Adélia da Costa Pereira de Arruda Neta, José Luiz de Brito Alves, Vinícius José Baccin Martins

DOI: 10.1210/jendso/bvaf011

Context: Obesity has been associated with changes in cortisol and dehydroepiandrosterone (DHEA) sulfate concentrations and increased stress levels and food addiction.

Objectives: We explored changes in morning salivary cortisol and DHEA in childhood obesity and their associations with body composition, metabolic profile, food addiction, food consumption, and stress in a cross-sectional study.

Methods: Children aged 7 to 12 years of both sexes were allocated into 2 groups according to body mass index-for-age: control group (n = 60) or obesity group (n = 98). Anthropometric, body composition, serum glucose, insulin, lipid profile, and DHEA were measured. Saliva was collected at different times to measure morning salivary cortisol concentrations. Food addiction, food consumption, and stress were assessed using questionnaires.

Results: Lower DHEA [1.04 (0.87-1.25) ng/mL vs 1.65 (1.30-2.07) ng/mL, P = .002] and salivary cortisol (6:00 Am: 1.17 ± 0.89 vs 1.45 ± 0.82 nmol/L, 6:30 Am: 1.53 ± 0.68 vs 1.83 ± 0.70 nmol/L, 7:30 Am: 0.72 ± 0.99 vs 1.31 ± 0.94 nmol/L, P-value of time < 0.001 and P-value of group = .002) were observed in children with obesity compared to the control. DHEA correlated negatively with waist circumference (r = -0.20, P < .05), body mass index-for-age(BMI-Z) (r = -0.21, P < .01), and weight (r = -0.25, P < .01). DHEA showed a positive correlation with the cortisol area under the curve (r = 0.29, P = .002). Food addiction was positively correlated with waist circumference (r = 0.21, P < .01), BMI-Z (r = 0.22, P < .01), body weight (r = 0.20, P < .05), total energy intake (r = 0.20, P < .05), and lipids (r = 0.24, P < .01).

Conclusion: Children with obesity showed lower concentrations of salivary cortisol and DHEA and higher food addiction compared to control children. These changes may contribute to the development of chronic diseases over time.

Effectiveness and metabolic impacts of restricting the consumption of ultra-processed foods in individuals with obesity submitted to energy restriction: a randomized clinical trial

Mateus L Macena, Micnéias R Pereira, Dafiny R Silva, André E Silva-Júnior, Ana Debora S Oliveira, João Victor L Santos, Déborah T C Paula, Maria Bárbara Galdino-Silva, Karine M M Almeida, Débora C Ferro, Guilherme C O Carvalho, Marianna V C Rocha, Natália G S Lopes, Rodrigo T L Carnaúba, Samyra A M Carvalho, Ana P G Clemente, Gabriel S Bádue, Ingrid S V Melo, João A Barros-Neto, Telma M M T Florêncio, Vinícius J B Martins, Nassib B Bueno

DOI: 10.1016/j.numecd.2025.104426

Background and aims: To evaluate the effectiveness and metabolic effects of restricting UPF consumption in individuals with obesity undergoing energy restriction.

Methods and results: Randomized, parallel clinical trial, lasting 12 months. Participants were randomly allocated into two groups: (a) generic energy restriction (ER-G) and (b) energy restriction associated with UPF restriction (ER-UPF). Energy requirements were determined using calorimetry and accelerometry data. Anthropometric, dietary, body composition, metabolic, and biochemical data were collected. 148 individuals were included. The baseline intake of UPF was 21.16 [18.42; 23.90]% in the ER-UPF group and 23.70 [20.92; 26.48]% in the ER-G group, and, at 12 months, decreased to 13.86 % in the ER-UPF and to 20.02 % in the ER-G (p = 0.08). The ER-UPF group reduced the NOVA-UPF Score (from 2.74 [2.28; 3.20] to 1.86 [1.18; 2.53] at 12 months) compared to the ER-G (from 2.62 [2.15; 3.09] to 2.47 [1.76; 3.17]; p = 0.03). The monthly bodyweight data analysis showed that the ER-UPF group lost more weight compared to the ER-G group (final values: 82.9 [79.6; 86.2] kg vs. 86.3 [83.0; 89.7] kg; p = 0.01). Despite these findings, no changes were observed in the other outcomes.

Conclusion: The proposed intervention resulted in significantly smaller decreases in UPF intake than expected and induced only a statistically, but non-clinically significant, greater weight loss compared to the ER-G. These findings may be partially explained by the fact that individuals had low UPF intake in the baseline. Future studies should focus on populations with higher basal UPF intake.

A DECLARATION ON THE VALUE OF EXPERIENTIAL MEASURES OF FOOD AND WATER INSECURITY TO IMPROVE SCIENCE AND POLICIES IN LATIN AMERICA AND THE CARIBBEAN             (1475-9276). INTERNATIONAL JOURNAL FOR EQUITY IN HEALTH (ONLINE). 2023.

A KETOGENIC DIET PATTERN DURING LACTATION INCREASES VISCERAL FAT, GLUCOSE, AND CHOLESTEROL LEVELS AND REDUCES BENEFICIAL FECAL BACTERIA IN DAMS AND OFFSPRING (2212-4292). FOOD BIOSCIENCE. 2023.

A LONGITUDINAL ANALYSIS OF THE FLUCTUATION OF FOOD STORES IN BELO HORIZONTE, MINAS GERAIS, BRAZIL             (1471-2458). BMC PUBLIC HEALTH (ONLINE). 2023.

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OBESITY INFLUENCES THE DEVELOPMENT OF BISPHOSPHONATE-INDUCED OSTEONECROSIS IN WISTAR RATS (1678-7765). JOURNAL OF APPLIED ORAL SCIENCE (ONLINE). 2023.

PEPPERMINT ESSENTIAL OIL (MENTHA PIPERITA L.) INCREASES TIME TO EXHAUSTION IN RUNNERS (1436-6207). EUROPEAN JOURNAL OF NUTRITION. 2023.

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PROBIOTICS FOR NUTRITION RESEARCH IN HEALTH AND DISEASE (1664-302X). FRONTIERS IN MICROBIOLOGY (ONLINE). 2023.        

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CUTOFF POINTS IN THE WAIST-HEIGHT RATIO FOR THE PREDICTION OF INSULIN RESISTANCE, SECOND STAGE OF SEXUAL MATURATION IN BRAZILIAN ADOLESCENTS (0007-1145). BRITISH JOURNAL OF NUTRITION. 2022.

DIFFERENT ACQUISITION SYSTEMS FOR HEART RATE VARIABILITY ANALYSIS MAY LEAD TO DIVERSE OUTCOMES (1414-431X). BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH. 2022.

DNMT3B (RS2424913) POLYMORPHISM IS ASSOCIATED WITH SYSTEMIC LUPUS ERYTHEMATOSUS ALONE AND WITH CO-EXISTING PERIODONTITIS IN A BRAZILIAN POPULATION       (1678-7765). JOURNAL OF APPLIED ORAL SCIENCE (ONLINE). 2022.

DYNAMICS OF PHYSIOLOGICAL RESPONSES OF POTENTIALLY PROBIOTIC FRUIT-DERIVED LIMOSILACTOBACILLUS FERMENTUM IN APPLE AND ORANGE JUICES DURING REFRIGERATION STORAGE AND EXPOSURE TO SIMULATED GASTROINTESTINAL CONDITIONS           (0302-8933). ARCHIVES OF MICROBIOLOGY. 2022.

EFFECT OF CITRAL NANOEMULSION ON THE INACTIVATION OF LISTERIAMONOCYTOGENES AND SENSORY PROPERTIES OF FRESH-CUT MELON AND PAPAYA DURING STORAGE     (0168-1605). INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. 2022.

EFFECTS OF BARU ALMOND OIL (DIPTERYX ALATA VOG.) TREATMENT ON THROMBOTIC PROCESSES, PLATELET AGGREGATION, AND VASCULAR FUNCTION IN AORTA ARTERIES (2072-6643). NUTRIENTS (BASEL). 2022.

EFFECTS OF THE ECONOMIC RECESSION ON SUICIDE MORTALITY IN BRAZIL: INTERRUPTED TIME SERIES ANALYSIS (1984-0446). REVISTA BRASILEIRA DE ENFERMAGEM. 2022.         

EFFECTS OF THE TREATMENT WITH FLAVONOIDS ON METABOLIC SYNDROME COMPONENTS IN HUMANS: A SYSTEMATIC REVIEW FOCUSING ON MECHANISMS OF ACTION (1422-0067). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (ONLINE). 2022.

EMERGING TECHNOLOGIES IN FOOD PROCESSING: IMPACTS ON SENSORY CHARACTERISTICS AND CONSUMER PERCEPTION       (2214-7993). CURRENT OPINION IN FOOD SCIENCE. 2022.

EVALUATING THE STABILITY OF A NOVEL NUTRACEUTICAL FORMULATION COMBINING PROBIOTIC LIMOSILACTOBACILLUS FERMENTUM 296, QUERCETIN, AND RESVERATROL UNDER DIFFERENT STORAGE CONDITIONS            (1867-1306). PROBIOTICS AND ANTIMICROBIAL PROTEINS. 2022.

EVALUATION OF POTENTIALLY PROBIOTIC YEASTS AND LACTIPLANTIBACILLUS PLANTARUM IN CO-CULTURE FOR THE ELABORATION OF A FUNCTIONAL PLANT-BASED FERMENTED BEVERAGE (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

EVIDENCE FOR QUERCETIN AS A DIETARY SUPPLEMENT FOR THE TREATMENT OF CARDIO-METABOLIC DISEASES IN PREGNANCY: A REVIEW IN RODENT MODELS  (2304-8158). FOODS. 2022.    

EXTRA VIRGIN COCONUT OIL (COCOS NUCIFERA L.) EXERTS ANTI-OBESITY EFFECT BY MODULATING ADIPOSITY AND IMPROVES HEPATIC LIPID METABOLISM, LEPTIN AND INSULIN RESISTANCE IN DIET-INDUCED OBESE RATS (1756-4646). JOURNAL OF FUNCTIONAL FOODS. 2022.

FATTY ACIDS, ESSENTIAL AMINO ACIDS, MINERALS AND PROTEINS PROFILE IN WHEY OF GOAT CHEESE: IMPACTS OF RAISING SYSTEMS (0921-4488). SMALL RUMINANT RESEARCH. 2022.

FRUIT AND VEGETABLE CONSUMPTION ACROSS GENERATIONS OF PRIMARY BRAZILIAN CARE USERS               (0899-9007). NUTRITION. 2022.

FUNCTIONAL AND MORPHOLOGIC DYSFUNCTIONS IN THE AIRWAYS OF RATS SUBMITTED TO AN EXPERIMENTAL MODEL OF OBESITY-EXACERBATED ASTHMA             (2045-2322). SCIENTIFIC REPORTS. 2022.

GARLIC PROMOTES ACUTE BLOOD PRESSURE REDUCTION BUT DOES NOT ENHANCE THE POSTEXERCISE HYPOTENSIVE EFFECTL’AGLIO PROMUOVE LA RIDUZIONE ACUTA DELLA PRESSIONE ARTERIOSA MA NON POTENZIA L’EFFETTO IPOTENSIVO POST-ESERCIZIO (0025-7826). MEDICINA DELLO SPORT (TESTO STAMPATO). 2022.        

GENETIC POLYMORPHISMS OF GENES INVOLVED IN OXIDATIVE STRESS AND INFLAMMATORY MANAGEMENT IN ONCOPEDIATRIC PATIENTS WITH CHEMO-INDUCED ORAL MUCOSITIS (1678-7765). JOURNAL OF APPLIED ORAL SCIENCE (ONLINE). 2022.         

GROWTH BEHAVIOR OF LOW POPULATIONS OF LISTERIA MONOCYTOGENES ON FRESH-CUT MANGO, MELON AND PAPAYA UNDER DIFFERENT STORAGE TEMPERATURES               (0740-0020). FOOD MICROBIOLOGY (PRINT). 2022.

GROWTH POTENTIAL OF THREE STRAINS OF LISTERIA MONOCYTOGENES AND SALMONELLA ENTERICA IN FRESCAL AND SEMI-HARD ARTISANAL MINAS MICROCHEESES: IMPACT OF THE ADDITION OF LACTIC ACID BACTERIA WITH ANTIMICROBIAL ACTIVITY        (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2022.

HETEROZYGOTE ADVANTAGE OF THE TYPE II DEIODINASE THR92ALA POLYMORPHISM ON INTRAHOSPITAL MORTALITY OF COVID-19 (0021-972X). THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM. 2022.        

IN VITRO COLONIC FERMENTATION AND POTENTIAL PREBIOTIC PROPERTIES OF PRE-DIGESTED JABUTICABA (MYRCIARIA JABOTICABA (VELL.) BERG) BY-PRODUCTS (0308-8146). FOOD CHEMISTRY. 2022.

INCORPORATION OF PHENOLIC-RICH INGREDIENTS FROM INTEGRAL VALORIZATION OF ISABEL GRAPE IMPROVES THE NUTRITIONAL, FUNCTIONAL AND SENSORY CHARACTERISTICS OF PROBIOTIC GOAT MILK YOGURT (0308-8146). FOOD CHEMISTRY. 2022.

JUÁ (ZIZIPHUS JOAZEIRO MART.) MUCILAGE AND JUÁ BY-PRODUCT PHENOLIC EXTRACT IMPROVE QUALITY PARAMETERS AND RETAIN BIOACTIVE COMPOUNDS IN FRESH-CUT PINEAPPLE DURING STORAGE (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

LACTICASEIBACILLUS CASEI 01 IMPROVES THE SENSORY CHARACTERISTICS IN GOAT MILK YOGURT ADDED WITH XIQUE-XIQUE (PILOSOCEREUS GOUNELLEI) JAM THROUGH CHANGES IN VOLATILES CONCENTRATION        (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2022.

LACTOBACILLUSGROUP AND ARTERIAL HYPERTENSION: A BROAD REVIEW ON EFFECTS AND PROPOSED MECHANISMS (1040-8398). CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION. 2022.

LIMOSILACTOBACILLUS FERMENTUM, CURRENT EVIDENCE ON THE ANTIOXIDANT PROPERTIES AND OPPORTUNITIES TO BE EXPLOITED AS A PROBIOTIC MICROORGANISM (1867-1306). PROBIOTICS AND ANTIMICROBIAL PROTEINS. 2022.

LIMOSILACTOBACILLUS FERMENTUMPREVENT GUT-KIDNEY OXIDATIVE DAMAGE AND THE RISE IN BLOOD PRESSURE IN MALE RAT OFFSPRING EXPOSED TO A MATERNAL HIGH-FAT DIET (2040-1752). JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE. 2022.

LONGITUDINAL EFFECT OF NUTRITIONAL INTERVENTION ON BODY WEIGHT: A RANDOMIZED CONTROLLED TRIAL    (0899-9007). NUTRITION. 2022.           

LOW MUSCLE MASS AND HIGH VISCERAL FAT MASS PREDICT MORTALITY IN PATIENTS HOSPITALIZED WITH MODERATE-TO-SEVERE COVID-19: A PROSPECTIVE STUDY (2049-3614). ENDOCRINE CONNECTIONS. 2022.

MALAY APPLE (SYZYGIUM MALACCENSE) PROMOTES CHANGES IN LIPID METABOLISM AND A HEPATOPROTECTIVE EFFECT IN RATS FED A HIGH-FAT DIET (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.            

MALICIA HONEY (MIMOSA QUADRIVALVIS L.) PRODUCED BY THE JANDAÍRA BEE (MELIPONA SUBNITIDA D.) IMPROVES DEPRESSIVE-LIKE BEHAVIOUR, SOMATIC, BIOCHEMICAL AND INFLAMMATORY PARAMETERS OF OBESE RATS (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

METABOLIC IMPACT OF THE VDR RS1544410 IN DIABETIC RETINOPATHY (1932-6203). PLOS ONE. 2022.

MICROENCAPSULATION OF CYMBOPOGON CITRATUS D.C. STAPF ESSEN-TIAL OIL WITH SPRAY DRYING: DEVELOPMENT, CHARACTERIZATION, AND ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES             (2304-8158). FOODS. 2022.  

MTHFR POLYMORPHISMS AND CARDIAC PARAMETERS IN PATIENTS WITH DIABETIC RETINOPATHY               (1573-3998). CURRENT DIABETES REVIEWS. 2022.   

MUSCULOSKELETAL EFFECTS OF OBESITY AND BARIATRIC SURGERY – A NARRATIVE REVIEW (2359-3997). ARCHIVES OF ENDOCRINOLOGY AND METABOLISM. 2022.

NOPAL BEVERAGE (OPUNTIA FICUS-INDICA) AS A NON-TRADITIONAL FOOD: SENSORY PROPERTIES, EXPECTATIONS, EXPERIENCES, AND EMOTIONS OF LOW-INCOME AND FOOD-INSECURE BRAZILIAN POTENTIAL CONSUMERS (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

NUCLEAR SCORE EVALUATION IN FOLLICULAR-PATTERNED THYROID LESIONS USING OPTICAL AND DIGITAL ENVIRONMENTS (1559-0100). ENDOCRINE. 2022.

NUTRACEUTICAL FORMULATIONS COMBINING LIMOSILACTOBACILLUS FERMENTUM, QUERCETIN, AND OR RESVERATROL WITH BENEFICIAL IMPACTS ON THE ABUNDANCE OF INTESTINAL BACTERIAL POPULATIONS, METABOLITE PRODUCTION, AND ANTIOXIDANT CAPACITY DURING COLONIC FERMENTATION (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

NUTRITIONAL CHARACTERIZATION, ANTIOXIDANT, AND LIPID-LOWERING EFFECTS OF YELLOW MOMBIN (SPONDIAS MOMBIN) SUPPLEMENTED TO RATS FED A HIGH-FAT DIET (2304-8158). FOODS. 2022.              

NUTRITIONAL, PHYSICOCHEMICAL AND STRUCTURAL PARAMETERS OF MAURITIA FLEXUOSA FRUITS AND BY-PRODUCTS FOR BIOTECHNOLOGICAL EXPLORATION OF SUSTAINABLE GOODS (1330-9862). FOOD TECHNOLOGY AND BIOTECHNOLOGY. 2022.

NUTRITIONAL, THERAPEUTIC, AND TECHNOLOGICAL PERSPECTIVES OF QUINOA ( CHENOPODIUM QUINOAWILLD.): A REVIEW (1745-4549). JOURNAL OF FOOD PROCESSING AND PRESERVATION. 2022.

PHYSICAL, NUTRITIONAL, AND BIOACTIVE PROPERTIES OF MANDACARU CLADODE FLOUR (CEREUS JAMACARU DC.): AN UNCONVENTIONAL FOOD PLANT FROM THE SEMI-ARID BRAZILIAN NORTHEAST               (2304-8158). FOODS.  2022.

PILOSOCEREUS GOUNELLEI (XIQUE-XIQUE) FLOUR: IMPROVING THE NUTRITIONAL, BIOACTIVE, AND TECHNOLOGICAL PROPERTIES OF PROBIOTIC GOAT-MILK YOGURT (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY            . 2022.

POLYMORPHISM BUT NOT METHYLATION STATUS IN THEVDRGENE CONTRIBUTES TO ORAL MUCOSITIS IN CHILDREN           (1354-523X). ORAL DISEASES. 2022. 

POTENTIAL ROLE OF LIMOSILACTOBACILLUS FERMENTUMAS A PROBIOTIC WITH ANTI-DIABETIC PROPERTIES: A REVIEW (1948-9358). WORLD JOURNAL OF DIABETES. 2022.           

POTENTIALLY PROBIOTIC LIMOSILACTOBACILLUS FERMENTUM FRUIT-DERIVED STRAINS ALLEVIATE CARDIOMETABOLIC DISORDERS AND GUT MICROBIOTA IMPAIRMENT IN MALE RATS FED A HIGH-FAT DIET (1867-1306). PROBIOTICS AND ANTIMICROBIAL PROTEINS. 2022.

PREBIOTICS IN NON-DAIRY PRODUCTS: TECHNOLOGICAL AND PHYSIOLOGICAL FUNCTIONALITY, CHALLENGES, AND PERSPECTIVES   (2212-4292). FOOD BIOSCIENCE. 2022.          

PROBIOTIC AND ANTIFUNGAL ATTRIBUTES OF LACTIC ACID BACTERIA ISOLATES FROM NATURALLY FERMENTED BRAZILIAN TABLE OLIVES             (2311-5637). FERMENTATION. 2022.

PROJECTED IMPACT OF CHANGE IN THE PERCENTAGE OF ENERGY FROM EACH NOVA GROUP INTAKE ON CARDIOVASCULAR DISEASE MORTALITY IN BRAZIL: A MODELLING STUDY         (2044-6055). BMJ OPEN. 2022.

PROJETO SAÚDE BRUMADINHO: INSEGURANÇA ALIMENTAR E NUTRICIONAL VERSUS CONDIÇÕES SOCIOECONÔMICAS E DIMENSÕES DO SISTEMA ALIMENTAR APÓS DESASTRE       (1415-790X). REVISTA BRASILEIRA DE EPIDEMIOLOGIA (IMPRESSO). 2022.

PURPLE GRAPE JUICE IMPROVES PERFORMANCE OF RECREATIONAL RUNNERS, BUT THE EFFECT IS GENOTYPE DEPENDENT: A DOUBLE BLIND, RANDOMIZED, CONTROLLED TRIAL(1555-8932). GENES & NUTRITION: A JOURNAL DEVOTED TO STUDY OF RELATIONSHIP BETWEEN GENETICS & NUTRITION FOR IMPROVEMENT OF HUMAN HEALTH. 2022.

REASONS FOR LIMITED WEIGHT LOSS IN PEOPLE WHO ENGAGE IN PHYSICAL TRAINING PROGRAMS: COMPARISON WITH A PHYSICALLY ACTIVE PROFESSION CAN SHED LIGHT ON THIS QUESTION    (0765-1597). SCIENCE & SPORTS. 2022.

RELAÇÃO DAS PRÁTICAS PARENTAIS COM SOBREPESO E OBESIDADE EM ADOLESCENTES ESCOLARES: ESTUDO DE CASO-CONTROLE (2178-8650). REVISTA BAIANA DE ENFERMAGEM. 2022.

SINGLE MOTHERS IN POVERTY IN BRAZIL: REPERCUSSIONS ON QUALITY OF LIFE AND ANXIETY FOR PREGNANT AND POSTPARTUM WOMEN (1087-5549). JOURNAL OF POVERTY. 2022.

SPIRULINA PLATENSIS BIOMASS ENHANCES THE PROLIFERATION RATE OF LACTOBACILLUS ACIDOPHILUS 5 (LA-5) AND COMBINED WITH LA-5 IMPACT THE GUT MICROBIOTA OF MEDIUM-AGE HEALTHY INDIVIDUALS THROUGH AN IN VITRO GUT MICROBIOME MODEL (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.   

STINGLESS BEE HONEY: AN OVERVIEW OF HEALTH BENEFITS AND MAIN MARKET CHALLENGES  (1745-4514). JOURNAL OF FOOD BIOCHEMISTRY. 2022.     

SUPPLEMENTATION WITH SPIRULINA PLATENSIS IMPROVES TRACHEAL REACTIVITY IN WISTAR RATS MODULATED BY INFLAMMATION, NITRITE AND OXIDATIVE STRESS (1663-9812). FRONTIERS IN PHARMACOLOGY. 2022.          

SUPPLEMENTATION WITH SPIRULINA PLATENSIS PREVENTS DAMAGE TO RAT ERECTIONS IN A MODEL OF ERECTILE DYSFUNCTION PROMOTED BY HYPERCALORIC DIET-INDUCED OBESITY (1660-3397). MARINE DRUGS. 2022.

SURVIVAL DURING LONG-TERM STORAGE, MEMBRANE INTEGRITY, AND ULTRASTRUCTURAL ASPECTS OF LACTOBACILLUS ACIDOPHILUS 05 AND LACTICASEIBACILLUS CASEI 01 FREEZE-DRIED WITH FRESHWATER MICROALGAE BIOMASSES (0963-9969). FOOD RESEARCH INTERNATIONAL. 2022.

SURVIVAL KINETICS, MEMBRANE INTEGRITY AND METABOLIC ACTIVITY OF SALMONELLA ENTERICA IN CONVENTIONALLY AND OSMOTICALLY DEHYDRATED COCONUT FLAKES (0168-1605). INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. 2022.  

THE COVID-19 PANDEMIC IN A BRAZILIAN METROPOLIS: REPERCUSSION ON FOOD PRICES (1678-4464). CADERNOS DE SAÚDE PÚBLICA. 2022.

THE DIRECT AND INDIRECT COSTS OF CARDIOVASCULAR DISEASES IN BRAZIL (1932-6203). PLOS ONE. 2022.

THE EFFECT OF RESVERATROL IN CARDIO-METABOLIC DISORDERS DURING PREGNANCY AND OFFSPRING OUTCOMES: A REVIEW (2040-1744). JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE (PRINT). 2022. 

TRACKING OF DIETARY PATTERNS IN THE SECONDARY PREVENTION OF CARDIOVASCULAR DISEASE AFTER A NUTRITIONAL INTERVENTION PROGRAM-A RANDOMIZED CLINICAL TRIAL (2072-6643). NUTRIENTS (BASEL). 2022.

TRANSFER OF MS2 BACTERIOPHAGE FROM SURFACES TO RASPBERRY AND PITANGA FRUITS AND VIRUS SURVIVAL IN RESPONSE TO SANITIZATION, FROZEN STORAGE AND PRESERVATION TECHNOLOGIES (0740-0020). FOOD MICROBIOLOGY (PRINT). 2022.

YEAST CARBOXYMETHYL-GLUCAN IMPROVES ENDOTHELIAL FUNCTION AND INHIBITS PLATELET AGGREGATION IN SPONTANEOUSLY HYPERTENSIVE RATS (2042-650X). FOOD & FUNCTION. 2022.            

YEASTS FROM FERMENTED BRAZILIAN FRUITS AS BIOTECHNOLOGICAL TOOLS FOR INCREASING PHENOLICS BIOACCESSIBILITY AND IMPROVING THE VOLATILE PROFILE IN DERIVED PULPS (0308-8146). FOOD CHEMISTRY. 2022.

A REVIEW OF RECENT RESEARCH ON ANTIFUNGAL AGENTS AGAINST DERMATOPHYTE BIOFILMS (1460-2709). MEDICAL MYCOLOGY (OXFORD. ONLINE). 2021.       

A WORLDWIDE SYSTEMATIC REVIEW, META-ANALYSIS AND HEALTH RISK ASSESSMENT STUDY OF MYCOTOXINS IN BEERS (1541-4337). COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY. 2021.  

ABCG2 POLYMORPHISM, AGE AND LEUKOCYTE COUNT MAY CONTRIBUTE TO ORAL MUCOSITIS IN ONCOPEDIATRIC PATIENTS (1806-4760). BRAZILIAN DENTAL JOURNAL. 2021.

AFLATOXIN M IN BRAZILIAN GOAT MILK AND HEALTH RISK ASSESSMENT (0360-1234). JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH. PART B. PESTICIDES, FOOD CONTAMINANTS, AND AGRICULTURAL WASTES. 2021.

AMERICAN PALE ALE CRAFT BEER: INFLUENCE OF BREWER’S YEAST STRAINS ON THE CHEMICAL COMPOSITION AND ANTIOXIDANT CAPACITY (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

ANTIFUNGAL EFFECTS OF CONYZA BONARIENSIS(L.) CRONQUIST ESSENTIAL OIL AGAINST PATHOGENIC COLLETOTRICHUM MUSAEAND ITS INCORPORATION IN GUM ARABIC COATING TO REDUCE ANTHRACNOSE DEVELOPMENT IN BANANA DURING STORAGE (1364-5072). JOURNAL OF APPLIED MICROBIOLOGY (PRINT). 2021.

APPLICATION OF POTENTIALLY PROBIOTIC FRUIT-DERIVED LACTIC ACID BACTERIA LOADED INTO SODIUM ALGINATE COATINGS TO CONTROL ANTHRACNOSE DEVELOPMENT IN GUAVA AND MANGO DURING STORAGE  (1867-1306) PROBIOTICS AND ANTIMICROBIAL PROTEINS. 2021.

ASSOCIATION BETWEEN VALUES OF ANTHROPOMETRIC INDICATORS, TOTAL ANTIOXIDANT CAPACITY AND MALONDIALDEHYDE IN ADULTS: A POPULATION-BASED STUDY  (0211-6057). NUTRICION CLINICA Y DIETETICA HOSPITALARIA. 2021.        

AUTHENTICITY OF PROBIOTIC FOODS AND DIETARY SUPPLEMENTS (1664-302X). FRONTIERS IN MICROBIOLOGY (ONLINE). 2021.  

AVALIAÇÃO DE FATORES DE RISCO ASSOCIADOS À DEFICIÊNCIA DE VITAMINA A EM IDOSOS INSTITUCIONALIZADOS DA CIDADE DE JOÃO PESSOA – PB     (1517-2473). ESTUDOS INTERDISCIPLINARES SOBRE O ENVELHECIMENTO. 2021.

BIOTRANSFORMATION OF THE BRAZILIAN CAATINGA FRUIT-DERIVED PHENOLICS BY LACTOBACILLUS ACIDOPHILUS LA-5 AND LACTICASEIBACILLUS CASEI 01 IMPACTS BIOACCESSIBILITY AND ANTIOXIDANT ACTIVITY (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.

CARBOXYMETHYL-GLUCAN FROM REDUCES BLOOD PRESSURE AND IMPROVES BAROREFLEX SENSITIVITY IN SPONTANEOUSLY HYPERTENSIVE RATS (2042-650X). FOOD & FUNCTION. 2021.

CHOLINE METABOLITES, HYDROXYBUTYRATE AND HDL AFTER DIETARY FIBER SUPPLEMENTATION IN OVERWEIGHT/OBESE HYPERTENSIVE WOMEN: A METABOLOMIC STUDY           (2072-6643). NUTRIENTS (BASEL). 2021.

COCONUT OIL SUPPLEMENTATION DOES NOT AFFECT BLOOD PRESSURE VARIABILITY AND OXIDATIVE STRESS: A PLACEBO-CONTROLLED CLINICAL STUDY IN STAGE-1 HYPERTENSIVE PATIENTS (2072-6643). NUTRIENTS (BASEL). 2021.

COMBINED EFFECT OF ESSENTIAL OILS AND EMERGING TECHNOLOGIES ON FOOD SAFETY AND QUALITY          (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

COMBINED TRAINING IS NOT SUPERIOR TO STRENGHT AND AEROBIC TRAINING TO MITIGATE CARDIOVASCULAR RISK IN ADULT HEALTH MEN (0860-021X). BIOLOGY OF SPORT. 2021.

CONDUTAS QUE PODEM INTERFERIR NA FASE PRÉ- ANALÍTICA DO EXAME SUMÁRIO DE URINA            (2176-9133). COGITARE ENFERMAGEM. 2021.

CORONARY HEART DISEASE AND STROKE MORTALITY TRENDS IN BRAZIL 2000-2018 (1932-6203). PLOS ONE. 2021.        

CURRENT ADVANCES ON THE DEVELOPMENT AND APPLICATION OF PROBIOTIC-LOADED EDIBLE FILMS AND COATINGS FOR THE BIOPROTECTION OF FRESH AND MINIMALLY PROCESSED FRUIT AND VEGETABLES (2304-8158). FOODS. 2021.

DEVELOPMENT AND IN VITRO EVALUATION OF NOVEL NUTRACEUTICAL FORMULATIONS COMPOSED OF LIMOSILACTOBACILLUS FERMENTUM, QUERCETIN AND/OR RESVERATROL  (0308-8146). FOOD CHEMISTRY. 2021.

DIETARY FLAVONOID KAEMPFEROL REDUCES OBESITY-ASSOCIATED HYPOTHALAMIC MICROGLIA ACTIVATION AND PROMOTES BODY WEIGHT LOSS IN MICE WITH OBESITY (1476-8305). NUTRITIONAL NEUROSCIENCE (ONLINE). 2021.

DIFERENÇA DA QUALIDADE DE VIDA E INSEGURANÇA ALIMENTAR ENTRE HOMENS E MULHERES VIVENDO COM HIV/AIDS NO ESTADO DA PARAÍBA (1413-8123). CIÊNCIA E SAÚDE COLETIVA (IMPRESSO). 2021.        

DO ESSENTIAL OILS FROM PLANTS OCCURRING IN THE BRAZILIAN CAATINGA BIOME PRESENT ANTIFUNGAL POTENTIAL AGAINST DERMATOPHYTOSES? A SYSTEMATIC REVIEW (0175-7598). APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2021. 

DOES INTERMITTENT FASTING ASSOCIATED WITH AEROBIC TRAINING INFLUENCE PARAMETERS RELATED TO THE GUT-BRAIN AXIS OF WISTAR RATS? (0165-0327). JOURNAL OF AFFECTIVE DISORDERS (PRINT). 2021.    

DONKEY MILK AND FERMENTED DONKEY MILK: ARE THERE DIFFERENCES IN THE NUTRITIONAL VALUE AND PHYSICOCHEMICAL CHARACTERISTICS? (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

DURAÇÃO DO SONO, EXCESSO DE PESO E CONSUMO DE ALIMENTOS ULTRAPROCESSADOS EM ADOLESCENTES (1678-4561). CIÊNCIA & SAÚDE COLETIVA. 2021.

EDIBLE FLOWERS: DIVERSITY, BIOACTIVE COMPOUNDS, FUNCTIONALITY, SAFETY, AND QUALITY ISSUES (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.       

WHEAT-DURUM PASTA ADDED OF INACTIVATED DECREASES GLUCOSE AND TOTAL CHOLESTEROL LEVELS AND MODULATES GUT MICROBIOTA IN HEALTHY RATS.

EFEITOS DE DIETAS ISOENERGÉTICAS SOBRE A COMPOSIÇÃO CORPORAL E BALANÇO NITROGENADO DE PRATICANTES DE TREINAMENTO FUNCIONAL: UM ESTUDO PILOTO (1981-9919). REVISTA BRASILEIRA DE OBESIDADE, NUTRIÇÃO E EMAGRECIMENTO. 2021.

EFFECT OF COLD PLASMA ON AÇAI PULP: ENZYMATIC ACTIVITY, COLOR AND BIOACCESSIBILITY OF PHENOLIC COMPOUNDS (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

EFFECTS OF A MIXED LIMOSILACTOBACILLUS FERMENTUM FORMULATION WITH CLAIMED PROBIOTIC PROPERTIES ON CARDIOMETABOLIC VARIABLES, BIOMARKERS OF INFLAMMATION AND OXIDATIVE STRESS IN MALE RATS FED A HIGH-FAT DIET (2304-8158). FOODS. 2021.

EFFECTS OF A SINGLE ORAL MEGADOSE OF VITAMIN D3 ON INFLAMMATION AND OXIDATIVE STRESS MARKERS IN OVERWEIGHT AND OBESE WOMEN: A RANDOMIZED, DOUBLE-BLIND, PLACEBO-CONTROLLED CLINICAL TRIAL          (1178-7007). DIABETES, METABOLIC SYNDROME AND OBESITY: TARGETS AND THERAPY. 2021.

EFFECTS OF DIGESTED FLOURS FROM FOUR DIFFERENT SWEET POTATO (IPOMOEA BATATAS L.) ROOT VARIETIES ON THE COMPOSITION AND METABOLIC ACTIVITY OF HUMAN COLONIC MICROBIOTA IN VITRO (1750-3841). JOURNAL OF FOOD SCIENCE. 2021.        

EFFECTS OF HYPERLIPIDIC/ISOCALORIC DIETARY PROTOCOLS ON BODY COMPOSITION, PERFORMANCE, AND NITROGEN BALANCE IN STREET RUNNERS      (0765-1597). SCIENCE & SPORTS. 2021.

EFFECTS OF SUPPLEMENTATION OF TROPICAL FRUIT PROCESSING BY-PRODUCTS ON LIPID PROFILE, RETINOL LEVELS AND INTESTINAL FUNCTION IN WISTAR RATS (1678-2690). ANAIS DA ACADEMIA BRASILEIRA DE CIÊNCIAS (ONLINE). 2021.

EFFICACY OF POTENTIALLY PROBIOTIC FRUIT-DERIVED LACTOBACILLUS FERMENTUM, L. PARACASEI AND L. PLANTARUM TO REMOVE AFLATOXIN M1 IN VITRO (2072-6651). TOXINS. 2021.  

EMERGING TECHNOLOGIES FOR MYCOTOXINS REMOVAL FROM FOODS: RECENT ADVANCES, ROLES IN SUSTAINABLE FOOD CONSUMPTION, AND STRATEGIES FOR INDUSTRIAL APPLICATIONS (1745-4549). JOURNAL OF FOOD PROCESSING AND PRESERVATION. 2021.

EVALUATION OF ANTHROPOMETRY AS AN ALTERNATIVE TO DXA AS PREDICTOR OF LOW BONE MINERAL DENSITY IN CHILDREN AND ADOLESCENTS WITH CYSTIC FIBROSIS (2405-4577). CLINICAL NUTRITION ESPEN. 2021.

EVALUATION OF THE EFFECTIVENESS OF MACAÍBA PALM SEED KERNEL (ACROCOMIA INTUMESCENS DRUDE) ON ANXIOLYTIC ACTIVITY, MEMORY PRESERVATION AND OXIDATIVE STRESS IN THE BRAIN OF DYSLIPIDEMIC RATS (1932-6203). PLOS ONE.         2021.

EVALUATION OF THE IMPACT OF DIFFERENT DOSES OF CURCUMA LONGA L. ON ANTIOXIDANT CAPACITY: A RANDOMIZED, DOUBLE-BLIND, CROSSOVER PILOT TRIAL (2314-6141). BIOMED RESEARCH INTERNATIONAL. 2021.

FACTORS ASSOCIATED WITH HANDGRIP STRENGHT LOSS IN PEOPLE LIVING WITH HIV IN USE OF ANTIRETROVIRAL THERAPY (0211-6057). NUTRICION CLINICA Y DIETETICA HOSPITALARIA. 2021.  

FOOD ADDICTION SYMPTOMS AND METABOLIC CHANGES IN CHILDREN AND ADOLESCENTS WITH THE DOUBLE BURDEN OF MALNUTRITION (0007-1145). BRITISH JOURNAL OF NUTRITION. 2021.  
FRESHWATER MICROALGAE BIOMASSES EXERT PREBIOTIC EFFECT ON HUMAN COLONIC MICROBIOTA (2211-9264). ALGAL RESEARCH. 2021.

GENOMIC INVESTIGATION OF ANTIMICROBIAL RESISTANCE DETERMINANTS AND VIRULENCE FACTORS IN SALMONELLA ENTERICA SEROVARS ISOLATED FROM CONTAMINATED FOOD AND HUMAN STOOL SAMPLES IN BRAZIL (0168-1605). INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. 2021.

GUT MICROBIOTA: A POTENTIAL THERAPEUTIC TARGET FOR MANAGEMENT OF DIABETIC RETINOPATHY? (0024-3205). LIFE SCIENCES (1973).        2021. 

HEALTH BENEFITS AND TECHNOLOGICAL EFFECTS OF LACTICASEIBACILLUS CASEI-01: AN OVERVIEW OF THE SCIENTIFIC LITERATURE (0924-2244). TRENDS IN FOOD SCIENCE & TECHNOLOGY (REGULAR ED.). 2021.

IDENTIFICATION OF BIOACTIVE PEPTIDES RELEASED FROM IN VITRO GASTROINTESTINAL DIGESTION OF YAM PROTEINS (DIOSCOREA CAYENNENSIS)   (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.

IMPACT OF CASHEW (ANACARDIUM OCCIDENTALE L.) BY-PRODUCT ON COMPOSITION AND METABOLIC ACTIVITY OF HUMAN COLONIC MICROBIOTA IN VITRO INDICATES PREBIOTIC PROPERTIES (1432-0991). CURRENT MICROBIOLOGY. 2021.      

INSIGHTS INTO THE CURRENT EVIDENCE ON THE EFFECTS OF ESSENTIAL OILS TOWARD BENEFICIAL MICROORGANISMS IN FOODS WITH A SPECIAL EMPHASIS TO LACTIC ACID BACTERIA – A REVIEW        (0924-2244). TRENDS IN FOOD SCIENCE & TECHNOLOGY (REGULAR ED.). 2021.     

LIMOSILACTOBACILLUS FERMENTUM STRAINS WITH CLAIMED PROBIOTIC PROPERTIES EXERT ANTI-OXIDANT AND ANTI-INFLAMMATORY PROPERTIES AND PREVENT CARDIOMETABOLIC DISORDER IN FEMALE RATS FED A HIGH-FAT DIET (1867-1306). PROBIOTICS AND ANTIMICROBIAL PROTEINS. 2021.   

LIVE AND ULTRASOUND-INACTIVATED LACTICASEIBACILLUS CASEI MODULATE THE INTESTINAL MICROBIOTA AND IMPROVE BIOCHEMICAL AND CARDIOVASCULAR PARAMETERS IN MALE RATS FED A HIGH-FAT DIET (2042-650X). FOOD & FUNCTIOON. 2021.

LOW-FAT AND RICH-FIBERS MACAÚBA SAUCES: PHYSICAL AND OXIDATIVE STABILITY, NUTRITIONAL QUALITY AND SENSORY CHARCATERISTICS (2212-4292). FOOD BIOSCIENCE.            2021.  

MATERNAL CONSUMPTION OF -3 ATTENUATES METABOLIC DISRUPTION ELICITED BY SATURATED FATTY ACIDS ENRICHED-DIET IN OFFSPRING RATS (0939-4753). NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (TESTO STAMPATO). 2021.

METHYLATION PROFILE OF MIR-9-1 AND MIR-9-1/-9-3 AS POTENTIAL BIOMARKERS OF DIABETIC RETINOPATHY (1573-3998). CURRENT DIABETES REVIEWS. 2021. 

METODOLOGIA E INDICADORES PARA MONITORAMENTO DA NBCAL EM ESTABELECIMENTOS COMERCIAIS E SERVIÇOS DE SAÚDE: ESTUDO MULTICÊNTRICO (MULTI-NBCAL) (1678-4464). CADERNOS DE SAÚDE PÚBLICA. 2021.

MICROALGAE AS SOURCE OF FUNCTIONAL INGREDIENTS IN NEW-GENERATION FOODS: CHALLENGES, TECHNOLOGICAL EFFECTS, BIOLOGICAL ACTIVITY, AND REGULATORY ISSUES  (1040-8398). CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION. 2021.

MICROALGAE IN THE MEAT PROCESSING CHAIN: FEED FOR ANIMAL PRODUCTION OR SOURCE OF TECHNO-FUNCTIONAL INGREDIENTS (2214-7993). CURRENT OPINION IN FOOD SCIENCE.  2021.

MICROBIOLOGICAL, IMMUNOLOGICAL, AND HISTOLOGICAL CHANGES IN THE GUT OF SALMONELLA ENTERITIDIS-CHALLENGED RATS FED GOAT CHEESE CONTAINING LACTOBACILLUS RHAMNOSUS EM1107 (0022-0302). JOURNAL OF DAIRY SCIENCE. 2021.

MUCUNA PRURIENS TREATMENT SHOWS ANTI-OBESITY AND INTESTINAL HEALTH EFFECTS ON OBESE RATS (2042-650X). FOOD & FUNCTIOON. 2021.

MYCOTOXINS IN ARTISANAL BEERS: AN OVERVIEW OF RELEVANT ASPECTS OF THE RAW MATERIAL, MANUFACTURING STEPS AND REGULATORY ISSUES INVOLVED           (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.

NUTRITIONAL, PHYSICOCHEMICAL AND SENSORIAL ACCEPTANCE OF FUNCTIONAL COOKIES ENRICHED WITH XIQUEXIQUE (PILOSOCEREUS GOUNELLEI) FLOUR (1932-6203). PLOS ONE. 2021.  

NUTRITIONAL POTENTIAL AND BIOACTIVE COMPOUNDS OF XIQUE-XIQUE JUICE: AN UNCONVENTIONAL FOOD PLANT FROM SEMIARID BRAZILIAN (1745-4549). JOURNAL OF FOOD PROCESSING AND PRESERVATION. 2021.

ORANGE JUICE AND YOGURT CARRYING PROBIOTIC BACILLUS COAGULANS GBI-30 6086: IMPACT OF INTAKE ON WISTAR MALE RATS HEALTH PARAMETERS AND GUT BACTERIAL DIVERSITY    (1664-302X). FRONTIERS IN MICROBIOLOGY (ONLINE). 2021.

PARAPROBIOTICS OBTAINED BY SIX DIFFERENT INACTIVATION PROCESSES: IMPACTS ON THE BIOCHEMICAL PARAMETERS AND INTESTINAL MICROBIOTA OF WISTAR MALE RATS (0963-7486). INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION. 2021.

PHENOLIC-RICH EXTRACTS FROM ACEROLA, CASHEW APPLE AND MANGO BY-PRODUCTS CAUSE DIVERSE INHIBITORY EFFECTS AND CELL DAMAGES ON ENTEROTOXIGENIC ESCHERICHIA COLI (0266-8254). LETTERS IN APPLIED MICROBIOLOGY. 2021.

PHYSICAL ACTIVITY LEVEL INFLUENCES MTHFR GENE METHYLATION PROFILE IN DIABETIC PATIENTS (1664-042X). FRONTIERS IN PHYSIOLOGY. 2021.

PHYSICOCHEMICAL CHARACTERISTICS AND BIOACTIVE COMPOUNDS OF THE XIQUE-XIQUE (PILOSOCEREUS GOUNELLEI) CACTUS FROM CAATINGA BRAZILIAN: ARE THEY NUTRITIVE AND FUNCTIONAL? (2193-4134). JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION. 2021.

PHYSICOCHEMICAL PARAMETERS, PHYTOCHEMICAL PROFILE AND ANTI-OXIDANT PROPERTIES OF A NEW BEVERAGE FORMULATED WITH XIQUE-XIQUE (PILOSOCEREUS GOUNELLEI) CLADODE JUICE (2304-8158). FOODS. 2021.          

PILOSOCEREUS GOUNELLEI (XIQUE-XIQUE) JAM IS SOURCE OF FIBERS AND MINERAL AND IMPROVES THE NUTRITIONAL VALUE AND THE TECHNOLOGICAL PROPERTIES OF GOAT MILK YOGURT (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

POSTBIOTICS – WHEN SIMPLIFICATION FAILS TO CLARIFY (1759-5045). NATURE REVIEWS. GASTROENTEROLOGY & HEPATOLOGY (PRINT). 2021. 

POTENTIALLY OBESOGENIC DIETS ALTER METABOLIC AND NEUROBEHAVIOURAL PARAMETERS IN WISTAR RATS: A COMPARISON BETWEEN TWO DIETARY MODELS  (0165-0327). JOURNAL OF AFFECTIVE DISORDERS (PRINT). 2021.     

POTENTIATION OF ANTIFUNGAL ACTIVITY OF TERBINAFINE BY DIHYDROJASMONE AND TERPINOLENE AGAINST DERMATOPHYTES (0266-8254). LETTERS IN APPLIED MICROBIOLOGY. 2021.

PPAR-Α GENE IS INVOLVED IN BODY COMPOSITION VARIATION IN RESPONSE TO AN AEROBIC TRAINING PROGRAM IN OVERWEIGHT / OBESE (1687-4757). PPAR RES. 2021.

PRACTICAL APPLICABILITY OF GENETICS FOR THE PREVENTION AND TREATMENT OF HYPERTENSION (1524-6175). THE JOURNAL OF CLINICAL HYPERTENSION (GREENWICH, CONN.) 2021.

PRE AND POST-OPERATORY ANTHROPOMETRIC AND LABORATORY DIFFERENCES OF PATIENTS SUBMITTED TO ROUX-EN-Y GASTRIC BYPASS AND SLEEVE GASTRECTOMY (0716-1549). REVISTA CHILENA DE NUTRICION. 2021.

PREVALÊNCIA DE HIPOVITAMINOSE D E ASSOCIAÇÕES COM PARÂMETROS METABÓLICOS EM TRABALHADORES   (2176-7114). REVISTA CONTEXTO & SAÚDE (ONLINE). 2021.

PROBIOTIC ICE CREAM: A LITERATURE OVERVIEW OF THE TECHNOLOGICAL AND SENSORY ASPECTS AND HEALTH PROPERTIES (1471-0307). INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY (ONLINE). 2021.

PROBIOTIC NON-DAIRY FROZEN DESSERT: TECHNOLOGICAL AND SENSORY ASPECTS AND CHALLENGES (0924-2244). TRENDS IN FOOD SCIENCE & TECHNOLOGY (REGULAR ED.). 2021.

PROBIOTIC PROPERTIES OF YEASTS ISOLATED FROM BRAZILIAN FERMENTED TABLE OLIVES  (1364-5072). JOURNAL OF APPLIED MICROBIOLOGY (PRINT). 2021.     

PROBIOTICS AND PREBIOTICS: POTENTIAL PREVENTION AND THERAPEUTIC TARGET FOR NUTRITIONAL MANAGEMENT OF COVID-19? (0954-4224). NUTRITION RESEARCH REVIEWS. 2021.

PRODUCTION AND CHARACTERIZATION OF EMULSIFIED FISH MORTADELLA FROM NILE TILAPIA (OREOCHROMUS NILOTICUS) (2182-1054). INTERNATIONAL JOURNAL OF FOOD STUDIES. 2021.

PROSPECTIVE ASSOCIATION BETWEEN DIETARY PATTERNS AND BMI Z-SCORE IN BRAZILIAN ADOLESCENTS (1368-9800). PUBLIC HEALTH NUTRITION (WALLINGFORD). 2021.

QUANTIFYING THE IMPACT OF EIGHT UNIT OPERATIONS ON THE SURVIVAL OF EIGHT BACILLUS STRAINS WITH CLAIMED PROBIOTIC PROPERTIES (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.      

RELATIONSHIP BETWEEN BSMI POLYMORPHISM AND VDR GENE METHYLATION PROFILE, GENDER, METABOLIC PROFILE, OXIDATIVE STRESS, AND INFLAMMATION IN ADOLESCENTS (1699-5198). NUTRICIÓN HOSPITALARIA. 2021.   

RELATIONSHIP OF THE PRO12ALA POLYMORPHISM ON THE PPARY2 GENE WITH THE BODY COMPOSITION OF PRACTITIONERS OF CYCLIC EXERCISES (1664-042X). FRONTIERS IN PHYSIOLOGY. 2021.

RUMINANT FAT INTAKE IMPROVES GUT MICROBIOTA, SERUM INFLAMMATORY PARAMETER AND FATTY ACID PROFILE IN TISSUES OF WISTAR RATS (2045-2322). SCIENTIFIC REPORTS. 2021.                        

SELECTION OF LACTIC ACID BACTERIA WITH IN VITRO PROBIOTIC-RELATED CHARACTERISTICS FROM THE CACTUS PILOSOCEREUS GOUNELLEI (A. WEBER EX. K. SCHUM.) BLY. EX ROWL (2304-8158). FOODS. 2021. 

SELECTION OF LACTIC ACID BACTERIA WITH PROMISING PROBIOTIC APTITUDES FROM FRUIT AND ABILITY TO SURVIVE IN DIFFERENT FOOD MATRICES (1517-8382). BRAZILIAN JOURNAL OF MICROBIOLOGY. 2021.       

SPREADABLE GOAT RICOTTA CHEESE ADDED WITH LACTOBACILLUS ACIDOPHILUS LA-05: CAN MICROENCAPSULATION IMPROVE THE PROBIOTIC SURVIVAL AND THE QUALITY PARAMETERS?         (0308-8146). FOOD CHEMISTRY. 2021.

STINGLESS BEE HONEY: AN OVERVIEW OF HEALTH BENEFITS AND MAIN MARKET CHALLENGES           (1745-4514). JOURNAL OF FOOD BIOCHEMISTRY. 2021.  

SUPPLEMENTATION WITH SPIRULINA PLATENSIS PREVENTS UTERINE DISEASES RELATED TO MUSCLE REACTIVITY AND OXIDATIVE STRESS IN RATS UNDERGOING STRENGTH TRAINING            (2072-6643). NUTRIENTS (BASEL). 2021.

THE PREVALENCE OF DERMATOPHYTOSES IN BRAZIL: A SYSTEMATIC REVIEW (0022-2615). JOURNAL OF MEDICAL MICROBIOLOGY. 2021.

THE ROLE OF VITAMIN B12 IN VIRAL INFECTIONS: A COMPREHENSIVE REVIEW OF ITS RELATIONSHIP WITH THE MUSCLE-GUT-BRAIN AXIS AND IMPLICATIONS FOR SARS-COV-2 INFECTION (0029-6643). NUTRITION REVIEWS. 2021.

THE USE OF ALTERNATIVE FOOD SOURCES TO IMPROVE HEALTH AND GUARANTEE ACCESS AND FOOD INTAKE (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.

THYROID HORMONE LEVELS DURING HOSPITAL ADMISSION INFORM DISEASE SEVERITY AND MORTALITY IN COVID-19 PATIENTS (1050-7256). THYROID (NEW YORK, N.Y.). 2021.

TRADITIONAL AND FLAVORED KOMBUCHAS WITH PITANGA AND UMBU-CAJÁ PULPS: CHEMICAL PROPERTIES, ANTIOXIDANTS, AND BIOACTIVE COMPOUNDS (2212-4292). FOOD BIOSCIENCE. 2021.

TYPICAL BRAZILIAN CHEESES: SAFETY, MINERAL CONTENT AND ADEQUACY TO THE NUTRITIONAL LABELING (1678-457X). CIENCIA E TECNOLOGIA DE ALIMENTOS. 2021.

ULTRASOUND TREATMENT FOR IMPROVING THE BIOACTIVE COMPOUNDS AND QUALITY PROPERTIES OF A BRAZILIAN NOPAL (OPUNTIA FICUS-INDICA) BEVERAGE DURING SHELF-LIFE (0023-6438). LEBENSMITTEL-WISSENSCHAFT + TECHNOLOGIE / FOOD SCIENCE + TECHNOLOGY. 2021.

UNDERSTANDING THE POTENTIAL OF FRUITS, FLOWERS, AND ETHNIC BEVERAGES AS VALUABLE SOURCES OF TECHNO-FUNCTIONAL AND PROBIOTICS STRAINS: CURRENT SCENARIO AND MAIN CHALLENGES       (0924-2244). TRENDS IN FOOD SCIENCE & TECHNOLOGY (REGULAR ED.). 2021.

USE OF SIX DIFFERENT INACTIVATION METHODS TO OBTAIN PARAPROBIOTICS: THEIR IMPACTS ON THE CULTIVABILITY, INTEGRITY, PHYSIOLOGY, AND MORPHOLOGY OF PROBIOTIC LACTOBACILUS ACIDOPHILUS, LACTICASEIBACILLUS CASEI AND BIFIDOBACTERIUM ANIMALIS      (1756-4646). JOURNAL OF FUNCTIONAL FOODS. 2021.

VEGAN PROBIOTIC PRODUCTS: A MODERN TENDENCY OR THE NEWEST CHALLENGE IN FUNCTIONAL FOODS          (0963-9969). FOOD RESEARCH INTERNATIONAL. 2021.  

VITAMIN D IN THE PREOPERATIVE AND POSTOPERATIVE PERIODS OF BARIATRIC SURGERY (0960-8923). OBESITY SURGERY. 2021.                            

Em Breve.